Reviewed, UniProtKB/Swiss-Prot Q4L6C5 (ARLS_STAHJ)

Names and origin

Protein names

Recommended name:
    Signal transduction histidine-protein kinase arlS
    EC=2.7.13.3

Gene names

Name:	arlS
Ordered Locus Names:	SH1491

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system arlS/arlR. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to arlR By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Signal transduction histidine-protein kinase arlS		PRO_0000293451
Regions
	Transmembrane	14 – 34	21	Potential
	Transmembrane	157 – 177	21	Potential
	Domain	179 – 232	54	HAMP
	Domain	240 – 453	214	Histidine kinase
Amino acid modifications
	Modified residue	243	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L6C5-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: D8021E99B1E335BF	FASTA	453	52,861
	10 20 30 40 50 60 MIKKGTLKYK WMMITTLIMF STIILFCLVI IFFLKDTLRD GEIDEAEHSS SEIVNLVESR 70 80 90 100 110 120 SMNNITTLDL TAMLENFEKA IIYDRNGKQL MQSSNENMIN FKPDIDFVDP ETIQISKHNG 130 140 150 160 170 180 IPYLIITEPI HSERFEGYSV LIHSLEGYNN VVRSLYFVAI AFGLLATFIM AGISYIFSTQ 190 200 210 220 230 240 LTKPLVTMSN KMIQIRRDGF QNKLELKTNY EETDNLIDTF NDMMYQIEES FNQQRQFVED 250 260 270 280 290 300 ASHELRTPLQ IIQGHLNLIQ RWGKKDPAVL EESLNISLEE MNRITKLVEE LLLLTKDKVN 310 320 330 340 350 360 IQALEFEEVN INEEIRSRIK SLKQLHPDYQ FKTHLSKKPL TLQINRHQFE QLLLIFIDNA 370 380 390 400 410 420 MKYDKDNKQI EIATQLRNKQ ISIEITDHGL GIPKEDQEFI FDRFYRVDKS RSRSQGGNGL 430 440 450 GLFIAEKIVQ QYGGYITVDS EVNQYTTFKI IFK

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006716 Genomic DNA. Translation: BAE04800.1.
RefSeq	YP_253406.1.
3D structure databases
HSSP	HSSP built from PDB template 1ID0 based on UniProtKB P23837.
SMR	Q4L6C5. Positions 217-452.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4L6C5.
Genome annotation databases
GeneID	3482387.
GenomeReviews	Gene locus SH1491 in contig AP006716_GR.
KEGG	sha:SH1491.
NMPDR	fig|279808.3.peg.2138.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0642.
HOGENOM	HBG694527.
OMA	FIDNAMK.
Enzyme and pathway databases
BioCyc	SHAE279808:SH1491-MONOMER.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLS_STAHJ

Accession

Primary (citable) accession number: Q4L6C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	August 2, 2005
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)