Q4L5V2 (TOP1_STAHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	SH1664

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	DNA topoisomerase 1		PRO_0000285946
Regions
	Domain	3 – 112	110	Toprim
	Zinc finger	577 – 603	27	C4-type 1
	Zinc finger	617 – 645	29	C4-type 2
	Zinc finger	658 – 681	24	C4-type 3
Sites
	Active site	298	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L5V2 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: C41C302CDCE30145	FASTA	689	79,373
	10 20 30 40 50 60 MADNLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDVEDNY EPKYITIRGK 70 80 90 100 110 120 GPVVKDLKKY AKKAKNVFLA SDPDREGEAI AWHLSKILEL DDSKENRVVF NEITKDAVKE 130 140 150 160 170 180 SFKHPRGIEM DLVDAQQARR ILDRLVGYNI SPVLWKKVKK GLSAGRVQSV ALRLVIDREN 190 200 210 220 230 240 EIRNFKPEEY WSIEGEFRYK KSKFTAKFLH YKNKPFKLKT KKDVEKVTAE LDGDKFEITN 250 260 270 280 290 300 VNKKEKTRNP ANPFTTSTLQ QEAARKLNFK ARKTMMLAQQ LYEGIDLKRQ GTVGLITYMR 310 320 330 340 350 360 TDSTRISQTA KDEAKQYIED KYGKDYLSNR TAKGKQGDQD AHEAIRPTST LRTPYEMKAY 370 380 390 400 410 420 LTRDQHRLYK LIWERFVASQ MAPAILDTVA LDVTQNNIKF RANGQTIKFK GFMTLYVEAK 430 440 450 460 470 480 DDKDNEKENK LPNLSKGDEV TATQIEPAQH FTQPPPRYTE ARLVKTLEEL KIGRPSTYAP 490 500 510 520 530 540 TIDTIQKRNY VKLESKRFVP TELGEIVYEQ VKDYFPEIID VEFTVNMETL LDKIAEGDIG 550 560 570 580 590 600 WRKVIDNFYG SFKLDVERAE EEMEKVEIKD EPAGEDCEVC GSPMVIKMGR YGKFMACSNF 610 620 630 640 650 660 PDCRNTKAIV KTIGVTCPKC KDGDVVERKS KKNRLFYGCS NYPECDFISW DKPVGRDCPK 670 680 CNHYLMEHKK GRSSQVICSN CDYKEEVQK

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCSC1435.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006716 Genomic DNA. Translation: BAE04973.1.
RefSeq	YP_253579.1. NC_007168.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortal	Q4L5V2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4L5V2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000045328; EBSTAP00000043779; EBSTAG00000045325.
GeneID	3481647.
GenomeReviews	Gene locus SH1664 in contig AP006716_GR.
KEGG	sha:SH1664.
NMPDR	fig|279808.3.peg.1036.
PATRIC	19619433. VBIStaHae67511_1640.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000024420.
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	Q4L5V2.
ProtClustDB	PRK05582.
Enzyme and pathway databases
BioCyc	SHAE279808:SH1664-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_STAHJ

Accession

Primary (citable) accession number: Q4L5V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program