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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi461MagnesiumUniRule annotation1
Metal bindingi467Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi470MagnesiumUniRule annotation1
Metal bindingi471MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
Short name:
PheRSUniRule annotation
Gene namesi
Name:pheTUniRule annotation
Ordered Locus Names:SH1822
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002320901 – 800Phenylalanine--tRNA ligase beta subunitAdd BLAST800

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi279808.SH1822.

Structurei

Secondary structure

1800
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Helixi5 – 8Combined sources4
Turni9 – 11Combined sources3
Helixi18 – 27Combined sources10
Beta strandi31 – 37Combined sources7
Turni38 – 41Combined sources4
Beta strandi43 – 54Combined sources12
Beta strandi60 – 62Combined sources3
Beta strandi64 – 68Combined sources5
Beta strandi70 – 73Combined sources4
Beta strandi75 – 78Combined sources4
Beta strandi89 – 99Combined sources11
Turni100 – 102Combined sources3
Beta strandi108 – 110Combined sources3
Beta strandi113 – 115Combined sources3
Beta strandi117 – 119Combined sources3
Helixi123 – 125Combined sources3
Helixi129 – 131Combined sources3
Helixi134 – 137Combined sources4
Helixi153 – 156Combined sources4
Beta strandi162 – 167Combined sources6
Helixi170 – 175Combined sources6
Helixi178 – 189Combined sources12
Beta strandi204 – 206Combined sources3
Helixi207 – 209Combined sources3
Beta strandi212 – 216Combined sources5
Turni218 – 220Combined sources3
Beta strandi222 – 230Combined sources9
Helixi239 – 247Combined sources9
Helixi255 – 267Combined sources13
Beta strandi272 – 275Combined sources4
Helixi276 – 278Combined sources3
Beta strandi279 – 288Combined sources10
Beta strandi294 – 297Combined sources4
Beta strandi302 – 304Combined sources3
Beta strandi310 – 321Combined sources12
Turni322 – 324Combined sources3
Beta strandi325 – 331Combined sources7
Beta strandi338 – 345Combined sources8
Helixi348 – 357Combined sources10
Helixi363 – 369Combined sources7
Helixi374 – 376Combined sources3
Helixi377 – 391Combined sources15
Beta strandi401 – 404Combined sources4
Beta strandi409 – 416Combined sources8
Helixi417 – 424Combined sources8
Helixi430 – 439Combined sources10
Beta strandi443 – 447Combined sources5
Beta strandi450 – 456Combined sources7
Helixi465 – 476Combined sources12
Turni478 – 480Combined sources3
Helixi498 – 512Combined sources15
Beta strandi522 – 524Combined sources3
Turni526 – 532Combined sources7
Beta strandi550 – 553Combined sources4
Helixi558 – 570Combined sources13
Beta strandi576 – 586Combined sources11
Beta strandi595 – 611Combined sources17
Helixi612 – 614Combined sources3
Beta strandi616 – 619Combined sources4
Helixi622 – 636Combined sources15
Beta strandi641 – 644Combined sources4
Beta strandi650 – 660Combined sources11
Beta strandi663 – 671Combined sources9
Helixi673 – 678Combined sources6
Beta strandi683 – 690Combined sources8
Helixi691 – 694Combined sources4
Beta strandi713 – 718Combined sources6
Beta strandi720 – 722Combined sources3
Helixi727 – 737Combined sources11
Helixi739 – 741Combined sources3
Beta strandi742 – 751Combined sources10
Beta strandi760 – 769Combined sources10
Beta strandi772 – 774Combined sources3
Helixi778 – 794Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RHQX-ray2.20B1-799[»]
2RHSX-ray2.20B/D1-800[»]
ProteinModelPortaliQ4L5E4.
SMRiQ4L5E4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4L5E4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 154tRNA-bindingUniRule annotationAdd BLAST116
Domaini408 – 483B5UniRule annotationAdd BLAST76
Domaini708 – 800FDX-ACBUniRule annotationAdd BLAST93

Sequence similaritiesi

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
Contains 1 B5 domain.UniRule annotation
Contains 1 FDX-ACB domain.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6A. Bacteria.
COG0072. LUCA.
COG0073. LUCA.
HOGENOMiHOG000292087.
KOiK01890.
OMAiMKFSEQW.
OrthoDBiPOG091H01YS.

Family and domain databases

CDDicd02796. tRNA_bind_bactPheRS. 1 hit.
Gene3Di3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR033714. tRNA_bind_bactPheRS.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4L5E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLISNEWLKD YVDAGVKVED LAERITRTGI EVDDMIDYSK DIKNLVVGYI
60 70 80 90 100
QSKEKHPDAD KLNICQVDIG EEEPVQIVCG APNVDAGQHV IVAKVGGRLP
110 120 130 140 150
GGIKIKRAKL RGERSEGMIC SLQEIGISSN VVPKAYENGI FVFQTEVEPG
160 170 180 190 200
TDALTALYLN DQVMEFDLTP NRADALSMVG TAYEVAALYQ TEMTKPETQS
210 220 230 240 250
NETSESATNE LSVTIDNPEK VPYYSARVVK NVSIEPSPIW VQARLIKAGI
260 270 280 290 300
RPINNVVDIS NYVLLEYGQP LHMFDQDHIG SKEIVVRQAK DEETMTTLDN
310 320 330 340 350
NERKLVDTDI VISNGQEPIA LAGVMGGDFS EVTEQTTNVV IEGAIFDPVS
360 370 380 390 400
IRHTSRRLNL RSEASSRFEK GIATEFVDEA VDRACYLLQE LASGEVLQDR
410 420 430 440 450
VSSGDLGSFV TPIDITAEKV NKTIGFNLSN DEIQSIFRQL GFETTLKGET
460 470 480 490 500
LTVNVPSRRK DITIKEDLIE EVARIYGYDE IPSSLPVFGE VTSGELTDRQ
510 520 530 540 550
HKTRTLKETL EGAGLNQAIT YSLVSKDHAK DFALQERPTI SLLMPMSEAH
560 570 580 590 600
ATLRQSLLPH LIEATAYNVA RKNKDVRLYE IGRVFFGNGE GELPDEVEYL
610 620 630 640 650
SGILTGEYVV NAWQGKKEEI DFFIAKGVVD RVAEKLNLEF SYKAGKIEGL
660 670 680 690 700
HPGRTAIVSL EGQDIGFIGE LHPQVAADND LKRTYVFELN YDAMMQVAVG
710 720 730 740 750
YINYEQIPKF PGVTRDIALE VNHDVPSSEL KQIIHNNGED ILQSTLVFDV
760 770 780 790 800
YEGEHLEKGK KSVAIRLNYL DTEDTLTDER VSKIHDKILE ALQAQGATIR
Length:800
Mass (Da):88,876
Last modified:August 2, 2005 - v1
Checksum:i7FBE75700E9AC989
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE05131.1.
RefSeqiWP_011276099.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE05131; BAE05131; SH1822.
GeneIDi24246528.
KEGGisha:SH1822.
PATRICi19619749. VBIStaHae67511_1797.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE05131.1.
RefSeqiWP_011276099.1. NC_007168.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RHQX-ray2.20B1-799[»]
2RHSX-ray2.20B/D1-800[»]
ProteinModelPortaliQ4L5E4.
SMRiQ4L5E4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279808.SH1822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE05131; BAE05131; SH1822.
GeneIDi24246528.
KEGGisha:SH1822.
PATRICi19619749. VBIStaHae67511_1797.

Phylogenomic databases

eggNOGiENOG4105C6A. Bacteria.
COG0072. LUCA.
COG0073. LUCA.
HOGENOMiHOG000292087.
KOiK01890.
OMAiMKFSEQW.
OrthoDBiPOG091H01YS.

Miscellaneous databases

EvolutionaryTraceiQ4L5E4.

Family and domain databases

CDDicd02796. tRNA_bind_bactPheRS. 1 hit.
Gene3Di3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR033714. tRNA_bind_bactPheRS.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFB_STAHJ
AccessioniPrimary (citable) accession number: Q4L5E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.