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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi461 – 4611MagnesiumUniRule annotation
Metal bindingi467 – 4671Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi470 – 4701MagnesiumUniRule annotation
Metal bindingi471 – 4711MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciSHAE279808:GJX7-1877-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
Short name:
PheRSUniRule annotation
Gene namesi
Name:pheTUniRule annotation
Ordered Locus Names:SH1822
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 800800Phenylalanine--tRNA ligase beta subunitPRO_0000232090Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi279808.SH1822.

Structurei

Secondary structure

1
800
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43Combined sources
Helixi5 – 84Combined sources
Turni9 – 113Combined sources
Helixi18 – 2710Combined sources
Beta strandi31 – 377Combined sources
Turni38 – 414Combined sources
Beta strandi43 – 5412Combined sources
Beta strandi60 – 623Combined sources
Beta strandi64 – 685Combined sources
Beta strandi70 – 734Combined sources
Beta strandi75 – 784Combined sources
Beta strandi89 – 9911Combined sources
Turni100 – 1023Combined sources
Beta strandi108 – 1103Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi117 – 1193Combined sources
Helixi123 – 1253Combined sources
Helixi129 – 1313Combined sources
Helixi134 – 1374Combined sources
Helixi153 – 1564Combined sources
Beta strandi162 – 1676Combined sources
Helixi170 – 1756Combined sources
Helixi178 – 18912Combined sources
Beta strandi204 – 2063Combined sources
Helixi207 – 2093Combined sources
Beta strandi212 – 2165Combined sources
Turni218 – 2203Combined sources
Beta strandi222 – 2309Combined sources
Helixi239 – 2479Combined sources
Helixi255 – 26713Combined sources
Beta strandi272 – 2754Combined sources
Helixi276 – 2783Combined sources
Beta strandi279 – 28810Combined sources
Beta strandi294 – 2974Combined sources
Beta strandi302 – 3043Combined sources
Beta strandi310 – 32112Combined sources
Turni322 – 3243Combined sources
Beta strandi325 – 3317Combined sources
Beta strandi338 – 3458Combined sources
Helixi348 – 35710Combined sources
Helixi363 – 3697Combined sources
Helixi374 – 3763Combined sources
Helixi377 – 39115Combined sources
Beta strandi401 – 4044Combined sources
Beta strandi409 – 4168Combined sources
Helixi417 – 4248Combined sources
Helixi430 – 43910Combined sources
Beta strandi443 – 4475Combined sources
Beta strandi450 – 4567Combined sources
Helixi465 – 47612Combined sources
Turni478 – 4803Combined sources
Helixi498 – 51215Combined sources
Beta strandi522 – 5243Combined sources
Turni526 – 5327Combined sources
Beta strandi550 – 5534Combined sources
Helixi558 – 57013Combined sources
Beta strandi576 – 58611Combined sources
Beta strandi595 – 61117Combined sources
Helixi612 – 6143Combined sources
Beta strandi616 – 6194Combined sources
Helixi622 – 63615Combined sources
Beta strandi641 – 6444Combined sources
Beta strandi650 – 66011Combined sources
Beta strandi663 – 6719Combined sources
Helixi673 – 6786Combined sources
Beta strandi683 – 6908Combined sources
Helixi691 – 6944Combined sources
Beta strandi713 – 7186Combined sources
Beta strandi720 – 7223Combined sources
Helixi727 – 73711Combined sources
Helixi739 – 7413Combined sources
Beta strandi742 – 75110Combined sources
Beta strandi760 – 76910Combined sources
Beta strandi772 – 7743Combined sources
Helixi778 – 79417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RHQX-ray2.20B1-799[»]
2RHSX-ray2.20B/D1-800[»]
ProteinModelPortaliQ4L5E4.
SMRiQ4L5E4. Positions 1-799.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ4L5E4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 154116tRNA-bindingUniRule annotationAdd
BLAST
Domaini408 – 48376B5UniRule annotationAdd
BLAST
Domaini708 – 80093FDX-ACBUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
Contains 1 B5 domain.UniRule annotation
Contains 1 FDX-ACB domain.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6A. Bacteria.
COG0072. LUCA.
COG0073. LUCA.
HOGENOMiHOG000292087.
KOiK01890.
OMAiMKFSEQW.
OrthoDBiEOG6CCH1J.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4L5E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLISNEWLKD YVDAGVKVED LAERITRTGI EVDDMIDYSK DIKNLVVGYI
60 70 80 90 100
QSKEKHPDAD KLNICQVDIG EEEPVQIVCG APNVDAGQHV IVAKVGGRLP
110 120 130 140 150
GGIKIKRAKL RGERSEGMIC SLQEIGISSN VVPKAYENGI FVFQTEVEPG
160 170 180 190 200
TDALTALYLN DQVMEFDLTP NRADALSMVG TAYEVAALYQ TEMTKPETQS
210 220 230 240 250
NETSESATNE LSVTIDNPEK VPYYSARVVK NVSIEPSPIW VQARLIKAGI
260 270 280 290 300
RPINNVVDIS NYVLLEYGQP LHMFDQDHIG SKEIVVRQAK DEETMTTLDN
310 320 330 340 350
NERKLVDTDI VISNGQEPIA LAGVMGGDFS EVTEQTTNVV IEGAIFDPVS
360 370 380 390 400
IRHTSRRLNL RSEASSRFEK GIATEFVDEA VDRACYLLQE LASGEVLQDR
410 420 430 440 450
VSSGDLGSFV TPIDITAEKV NKTIGFNLSN DEIQSIFRQL GFETTLKGET
460 470 480 490 500
LTVNVPSRRK DITIKEDLIE EVARIYGYDE IPSSLPVFGE VTSGELTDRQ
510 520 530 540 550
HKTRTLKETL EGAGLNQAIT YSLVSKDHAK DFALQERPTI SLLMPMSEAH
560 570 580 590 600
ATLRQSLLPH LIEATAYNVA RKNKDVRLYE IGRVFFGNGE GELPDEVEYL
610 620 630 640 650
SGILTGEYVV NAWQGKKEEI DFFIAKGVVD RVAEKLNLEF SYKAGKIEGL
660 670 680 690 700
HPGRTAIVSL EGQDIGFIGE LHPQVAADND LKRTYVFELN YDAMMQVAVG
710 720 730 740 750
YINYEQIPKF PGVTRDIALE VNHDVPSSEL KQIIHNNGED ILQSTLVFDV
760 770 780 790 800
YEGEHLEKGK KSVAIRLNYL DTEDTLTDER VSKIHDKILE ALQAQGATIR
Length:800
Mass (Da):88,876
Last modified:August 2, 2005 - v1
Checksum:i7FBE75700E9AC989
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE05131.1.
RefSeqiWP_011276099.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE05131; BAE05131; SH1822.
GeneIDi24246528.
KEGGisha:SH1822.
PATRICi19619749. VBIStaHae67511_1797.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE05131.1.
RefSeqiWP_011276099.1. NC_007168.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RHQX-ray2.20B1-799[»]
2RHSX-ray2.20B/D1-800[»]
ProteinModelPortaliQ4L5E4.
SMRiQ4L5E4. Positions 1-799.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279808.SH1822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE05131; BAE05131; SH1822.
GeneIDi24246528.
KEGGisha:SH1822.
PATRICi19619749. VBIStaHae67511_1797.

Phylogenomic databases

eggNOGiENOG4105C6A. Bacteria.
COG0072. LUCA.
COG0073. LUCA.
HOGENOMiHOG000292087.
KOiK01890.
OMAiMKFSEQW.
OrthoDBiEOG6CCH1J.

Enzyme and pathway databases

BioCyciSHAE279808:GJX7-1877-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ4L5E4.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species."
    Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K.
    J. Bacteriol. 187:7292-7308(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCSC1435.

Entry informationi

Entry nameiSYFB_STAHJ
AccessioniPrimary (citable) accession number: Q4L5E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 2, 2005
Last modified: March 16, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.