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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC)
  5. 2-succinylbenzoate--CoA ligase (menE)
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. no protein annotated in this organism
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processMenaquinone biosynthesis
LigandMagnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

UniPathwayiUPA00079
UPA01057; UER00164

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:SH1919
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003418651 – 5562-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseAdd BLAST556

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi279808.SH1919

Structurei

3D structure databases

ProteinModelPortaliQ4L547
SMRiQ4L547
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4A Bacteria
COG1165 LUCA
HOGENOMiHOG000218359
KOiK02551
OMAiIFRILPG
OrthoDBiPOG091H058U

Family and domain databases

HAMAPiMF_01659 MenD, 1 hit
InterProiView protein in InterPro
IPR029035 DHS-like_NAD/FAD-binding_dom
IPR004433 MenaQ_synth_MenD
IPR032264 MenD_middle
IPR029061 THDP-binding
IPR012001 Thiamin_PyroP_enz_TPP-bd_dom
IPR011766 TPP_enzyme-bd_C
PANTHERiPTHR42916 PTHR42916, 1 hit
PfamiView protein in Pfam
PF02775 TPP_enzyme_C, 1 hit
PF16582 TPP_enzyme_M_2, 1 hit
PF02776 TPP_enzyme_N, 1 hit
PIRSFiPIRSF004983 MenD, 1 hit
SUPFAMiSSF52467 SSF52467, 1 hit
SSF52518 SSF52518, 2 hits
TIGRFAMsiTIGR00173 menD, 1 hit

Sequencei

Sequence statusi: Complete.

Q4L547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHNEALTKQ VYTFASELYA YGVREVVISP GSRSTPLAIA FEAHPNIKTW
60 70 80 90 100
IHPDERSAAF FALGLIKGSE RPVAILCTSG TAAANYTPAI AESQISRIPL
110 120 130 140 150
IVLTSDRPHE LRSVGAPQAI NQVNMFANYV NFQFDMPVAD GTDYMLDAIY
160 170 180 190 200
YQMQIASQYL YGPHRGPIHF NLPFREPLTP NLERQEWLTS YSKKLPHYQK
210 220 230 240 250
NINVQDIRHI LKEKKGLIIV GDMQHQAIDQ ILTYATVHDI PILADPLSQL
260 270 280 290 300
RKYNHPNVIT TYDLYYRAGL DIDVDFVIRV GKPVISKKLN QWLKRTNAYQ
310 320 330 340 350
ILVQNNDKID VFPTPPSISY EISANDFFRN LIEEAAVDRK DWINMWRTLE
360 370 380 390 400
SQARNTIDKH MTNATDEAAF VKILIDKLTK DDAIFVSNSM PVRDIDNLLY
410 420 430 440 450
NSEVEVYANR GANGIDGVVS TAIGMAVHKK ITLIIGDLAF YHDMNGLLMA
460 470 480 490 500
KINNINLNIV LLNNDGGGIF SYLPQKASAE AYFERLFGTP TGLNFEYTAL
510 520 530 540 550
LYDFTFKRFN TVADFTQEKL SHVNSHIYEM VTNRDDNMSQ HQILYKKLSG

ILNVTL
Length:556
Mass (Da):63,095
Last modified:August 2, 2005 - v1
Checksum:iFE88BE461C5A72D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA Translation: BAE05228.1
RefSeqiWP_011276189.1, NC_007168.1

Genome annotation databases

EnsemblBacteriaiBAE05228; BAE05228; SH1919
GeneIDi24246494
KEGGisha:SH1919

Similar proteinsi

Entry informationi

Entry nameiMEND_STAHJ
AccessioniPrimary (citable) accession number: Q4L547
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: August 2, 2005
Last modified: April 25, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome