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Protein

D-alanine--D-alanyl carrier protein ligase

Gene

dltA

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.UniRule annotation

Catalytic activityi

D-alanine + ATP + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein].UniRule annotation

Pathwayi: lipoteichoic acid biosynthesis

This protein is involved in the pathway lipoteichoic acid biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway lipoteichoic acid biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189D-alanineUniRule annotation1
Binding sitei293D-alanine; via carbonyl oxygenUniRule annotation1
Binding sitei365ATPUniRule annotation1
Binding sitei473ATPUniRule annotation1
Binding sitei473D-alanineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi144 – 145ATPUniRule annotation2
Nucleotide bindingi284 – 289ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00556.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanyl carrier protein ligaseUniRule annotation (EC:6.2.1.-UniRule annotation)
Short name:
DCLUniRule annotation
Alternative name(s):
D-alanine--poly(phosphoribitol) ligase subunit 1UniRule annotation
D-alanine-activating enzymeUniRule annotation
Short name:
DAEUniRule annotation
Gene namesi
Name:dltAUniRule annotation
Ordered Locus Names:SH2021
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002131581 – 485D-alanine--D-alanyl carrier protein ligaseAdd BLAST485

Interactioni

Protein-protein interaction databases

STRINGi279808.SH2021.

Structurei

3D structure databases

ProteinModelPortaliQ4L4U5.
SMRiQ4L4U5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IQD. Bacteria.
COG1020. LUCA.
HOGENOMiHOG000229995.
KOiK03367.
OMAiNFYIIFT.
OrthoDBiPOG091H07SP.

Family and domain databases

HAMAPiMF_00593. DltA. 1 hit.
InterProiView protein in InterPro
IPR010071. AA_adenyl_domain.
IPR025110. AMP-bd_C.
IPR000873. AMP-dep_Synth/Lig.
IPR010072. DltA.
PfamiView protein in Pfam
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 1 hit.
TIGR01734. D-ala-DACP-lig. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4L4U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDIINLIND FGQSNPERVA VRHKDEELTY QQLMDESSKL AHLLQDNHKP
60 70 80 90 100
LIVYGHMSPY MLVGMIGAIK AGCGYVPIDT SVPSERVNMI INKVQPDIIF
110 120 130 140 150
NTSDTQLNHS NIQELTIQSI QDSDNPTLFD SQMGLTDVVY TIFTSGSTGE
160 170 180 190 200
PKGVQIEYAS LIEFAEWMVS LNESEGSQEW LNQAPFSFDL SVMAIYPCLT
210 220 230 240 250
SGGTLNLVDK EMINKPKLLN EMLVNTPINA WVSTPSFMEM CLLLPNLNES
260 270 280 290 300
SYPSLNHFFF CGEILPHRTA KALLDRYPSA VVYNTYGPTE ATVAVTGIKL
310 320 330 340 350
TPEVIEAYNP LPVGVSRPNT SLFTTDEGEL VIKGNSVSLG YLDNKEKTDA
360 370 380 390 400
VFNFEDGLRI YHTGDKAIEK DGQWFIQGRI DFQIKLNGYR MELEEIETQL
410 420 430 440 450
RQSEFVRETV VVPVYKNNKV IHLIGAVVPT EEVRDDLEMT RQIKSELKSR
460 470 480
LPEYMIPRKF VWMKQLPLTS NGKLDRKQVA EDINA
Length:485
Mass (Da):54,705
Last modified:August 2, 2005 - v1
Checksum:i6724289A0688ABB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE05330.1.
RefSeqiWP_011276287.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE05330; BAE05330; SH2021.
GeneIDi24246302.
KEGGisha:SH2021.

Similar proteinsi

Entry informationi

Entry nameiDLTA_STAHJ
AccessioniPrimary (citable) accession number: Q4L4U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: August 2, 2005
Last modified: November 22, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families