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Reviewed, UniProtKB/Swiss-Prot Q4KWH5 (PLCH1_MOUSE)

Last modified November 3, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase eta-1
    EC=3.1.4.11
Alternative name(s):
    Phospholipase C-eta-1
      Short name=PLC-eta-1
    Phospholipase C-like protein 3
      Short name=PLC-L3
Gene names
Name: Plch1
Synonyms: Kiaa1069, Plcl3
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length1682 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by calcium-activated phosphatidylinositol-specific phospholipase C enzymes. Ref.1

Catalytic activity

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactor

Calcium.

Subcellular location

Cytoplasm. Membrane By similarity.

Tissue specificity

Expressed in brain and to a lower extent in lung. In brain, it is found in cerebrum, cerebellum and spinal cord. Ref.1

Sequence similarities

Contains 1 C2 domain.

Contains 2 EF-hand domains.

Contains 1 PH domain.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

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Ontologies

Keywords
   Biological processLipid degradation
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Transducer
Gene Ontology (GO)
   Biological processintracellular signaling cascade

Inferred from electronic annotation. Source: InterPro

lipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytosol Ref.1

Inferred from direct assay. Source: MGI

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane fraction Ref.1

Inferred from direct assay. Source: MGI

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoinositide phospholipase C activity

Inferred from electronic annotation. Source: EC

signal transducer activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q4KWH5-1)

Also known as: PLC-eta-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q4KWH5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.
     557-557: Missing.
     863-882: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q4KWH5-3)

Also known as: PLC-eta-1b;

The sequence of this isoform differs from the canonical sequence as follows:
     1001-1073: AGDKDDRRKG...PNMTNDCQEN → DLNRKQRKQE...VQRVRGSNQI
     1074-1682: Missing.
Isoform 4 (identifier: Q4KWH5-4)

Also known as: PLC-eta-1a;

The sequence of this isoform differs from the canonical sequence as follows:
     1001-1003: AGD → VQI
     1004-1682: Missing.
Isoform 5 (identifier: Q4KWH5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-563: Missing.
     564-584: SKSYSTDDEDDSLQNPGKEGG → MDFFSLHFKTWAVTMSSCHQR
     863-882: Missing.
     1001-1073: AGDKDDRRKG...PNMTNDCQEN → DLNRKQRKQE...VQRVRGSNQI
     1074-1682: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 168216821-phosphatidylinositol-4,5-bisphosphate phosphodiesterase eta-1
PRO_0000329008

Regions

Domain20 – 128109PH
Domain142 – 17736EF-hand 1
Domain178 – 21437EF-hand 2
Domain299 – 444146PI-PLC X-box
Domain602 – 715114PI-PLC Y-box
Domain720 – 827108C2
Calcium binding155 – 16612 Potential
Compositional bias591 – 5944Poly-Arg
Compositional bias1027 – 10315Poly-Ser

Sites

Active site3141 By similarity
Active site3581 By similarity
Metal binding3151Calcium 1; catalytic By similarity
Metal binding3441Calcium 1; catalytic By similarity
Metal binding3461Calcium 1; catalytic By similarity
Metal binding3931Calcium 1; catalytic By similarity
Metal binding7591Calcium 2; via carbonyl oxygen By similarity
Metal binding7611Calcium 2 By similarity
Metal binding7851Calcium 2 By similarity
Metal binding8141Calcium 3 By similarity
Metal binding8151Calcium 3; via carbonyl oxygen By similarity
Metal binding8161Calcium 3 By similarity
Binding site4421Substrate By similarity
Binding site4441Substrate By similarity
Binding site6281Substrate By similarity
Binding site6551Substrate By similarity

Natural variations

Alternative sequence1 – 563563Missing in isoform 5.
VSP_032907
Alternative sequence1 – 2121Missing in isoform 2.
VSP_032908
Alternative sequence5571Missing in isoform 2.
VSP_032909
Alternative sequence564 – 58421SKSYS…GKEGG → MDFFSLHFKTWAVTMSSCHQ R in isoform 5.
VSP_032910
Alternative sequence863 – 88220Missing in isoform 2 and isoform 5.
VSP_032911
Alternative sequence1001 – 107373AGDKD…DCQEN → DLNRKQRKQETRMTEEREPQ LEKTHIFQISTKSYPPPPVR SSTKMPTKGQLPVYQTQNSV EDEVQRVRGSNQI in isoform 3 and isoform 5.
VSP_032912
Alternative sequence1001 – 10033AGD → VQI in isoform 4.
VSP_032913
Alternative sequence1004 – 1682679Missing in isoform 4.
VSP_032914
Alternative sequence1074 – 1682609Missing in isoform 3 and isoform 5.
VSP_032915

Experimental info

Sequence conflict373 – 38513VETIN…FVKNE → AIDRPWLCCCSLR in BAD32373. Ref.3
Sequence conflict5231H → D in BAD32373. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PLC-eta-1) [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 84B1BA6B4B6378A4

FASTA1,682187,743
        10         20         30         40         50         60 
MADLEVYKNL SPEKVERCMS VMQSGTQMIK LKRGTKGLVR LFYLDEHRTR LRWRPSRKSE 

        70         80         90        100        110        120 
KAKILIDSIY KVTEGRQSEI FHRQAEGNFD PSCCFTIYHG NHMESLDLIT SNPEEARTWI 

       130        140        150        160        170        180 
TGLKYLMAGI SDEDSLAKRQ RTHDQWVKQT FEEADKNGDG LLNIEEIHQL MHKLNVNLPR 

       190        200        210        220        230        240 
RKVRQMFQEA DTDENQGTLT FEEFCVFYKM MSLRRDLYLL LLSYSDKKDH LTVEELAQFL 

       250        260        270        280        290        300 
KVEQKMSNVT LDYCLDIIMK FEVSEENKVK NVLGIEGFTN FMRSPACDVF NPLHHEVYQD 

       310        320        330        340        350        360 
MDQPLCNYYI ASSHNTYLTG DQLLSQSKVD MYARVLQEGC RCVEVDCWDG PDGEPVVHHG 

       370        380        390        400        410        420 
YTLTSKILFR DVVETINKHA FVKNEFPVIL SIENHCSIQQ QRKIAQYLKG ILQDKLDLSS 

       430        440        450        460        470        480 
VDTGECRQLP SPQSLKGKIL VKGKKLPYHL GDDAEEGEVS DEDSADEIED ECKFKLHYSN 

       490        500        510        520        530        540 
GTTEHQVESF IRKKLESLLK ESQIRDKEDP DSFTVRALLK ATHEGLNAHL KQNLDVKESG 

       550        560        570        580        590        600 
KKSHGRSLMA NFGKHKQKAT KSRSKSYSTD DEDDSLQNPG KEGGQLYRLG RRRRTMKLCR 

       610        620        630        640        650        660 
ELSDLVVYTN SVAAQDIVDD GTTGNVLSFS ETRAHQVVQQ KSEQFMIYNQ KQLTRIYPSA 

       670        680        690        700        710        720 
YRIDSSNFNP LPYWNAGCQL VALNYQSEGR MMQINRAKFK ANGNCGYILK PQQMCKGTFN 

       730        740        750        760        770        780 
PFSGDPLPAN PKKQLILKVI SGQQLPKPPD SMFGDRGEII DPFVEVEIIG LPVDCCKDQT 

       790        800        810        820        830        840 
RVVDDNGFNP VWEETLTFTV HMPEIALVRF LVWDHDPIGR DFVGQRTVTF SSLVPGYRHV 

       850        860        870        880        890        900 
YLEGLTEASI FVHITINEIF GKWSPLILNP SYTILHFLGA TKNRQLQGLK GLFNKNPRHA 

       910        920        930        940        950        960 
SSENNSHYVR KRSIGDRILR RTASAPAKGR KKSKVGFQEM VEIKDSVSEA SRDQDGVLRR 

       970        980        990       1000       1010       1020 
TTRSLQVRPV SMPVDKSLLG ALSLPISEAA KDTDGKENCL AGDKDDRRKG AATRKDPHFS 

      1030       1040       1050       1060       1070       1080 
NFNKKLSSSS SALLHKDANQ GPTASVSNPE QCGGRGAKSE RIKPNMTNDC QENHNPPKFL 

      1090       1100       1110       1120       1130       1140 
SPRKHLALDP ATKGLQERLH GMKTNEKEHA EGFLGEKSML SGSVLSQSSL EVENLEGSRA 

      1150       1160       1170       1180       1190       1200 
KGRAATSFSL SDVSALCSDI PDLHSTAILQ DTEISNLIDD VTLTNENQSG SSISALIGQF 

      1210       1220       1230       1240       1250       1260 
EESNHPANVT VVSHLSTSGA SGSAPFQTPF KHGLSQGNQK ASFLCSSPEL NKLSSVETTK 

      1270       1280       1290       1300       1310       1320 
LANNAVPCGV IGSPISTPKP GDDPSDKAKT RVIEGNLPGF PDASPGQFPK SPTHGEDHSQ 

      1330       1340       1350       1360       1370       1380 
VMNSPALSTE LAIEDIIADP ALSINSAESS LVEIDGESEN LSLTTCDYRE EAPSQLVSPL 

      1390       1400       1410       1420       1430       1440 
KLQQSQEMVE HIQRGLRNGY CKETLLPSEI FNNIPGVKNH SISHLTYQGA GFVYNHFSSS 

      1450       1460       1470       1480       1490       1500 
DAKTNQICEP QQPRAPDMHA PTPTPSTHAP LAALKLPSPC KSKSLGDLTS EDIACNFESK 

      1510       1520       1530       1540       1550       1560 
YQCISRSFVT NGIRDKSVTM KTKSLEPLDA LTEQLRKLVS FDQEDSCQVL YSKQDVNQCP 

      1570       1580       1590       1600       1610       1620 
RALVRKLSSR SQSRVRNIAS RAKEKQEAGK QKAMAQSTRG GVVLRSKPPA PALAVNRHST 

      1630       1640       1650       1660       1670       1680 
GSYIASYLRN MKAGGLEGRG IPEGACTALR YGYMDQFCSD NSVLQTEPSS EDKPEIYFLL 


RL

« Hide

Isoform 2.

Checksum: CF855C1012C31490
Show »

FASTA1,640182,937
Isoform 3 (PLC-eta-1b).

Checksum: 8FCD04B59FF7E5A5
Show »

FASTA1,073122,771
Isoform 4 (PLC-eta-1a).

Checksum: 14A27D8A1271B93C
Show »

FASTA1,003114,547
Isoform 5.

Checksum: A256A8AE91E6F253
Show »

FASTA49055,818

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References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel phospholipase C, PLC-eta."
Hwang J.-I., Oh Y.-S., Shin K.-J., Kim H., Ryu S.H., Suh P.-G.
Biochem. J. 389:181-186(2005) [PubMed: 15702972] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3 AND 4), TISSUE SPECIFICITY, FUNCTION.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5).
Tissue: Eye.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 373-1682 (ISOFORM 2).
Tissue: Fetal brain.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AY691172 mRNA. Translation: AAW22609.1.
AY691173 mRNA. Translation: AAW22610.1.
AY691174 mRNA. Translation: AAW22611.1.
BC042549 mRNA. Translation: AAH42549.1.
BC052372 mRNA. Translation: AAH52372.1.
BC055005 mRNA. Translation: AAH55005.1.
AK173095 mRNA. Translation: BAD32373.1.
IPIIPI00229353.
IPI00338999.
IPI00620780.
IPI00622424.
IPI00890304.
RefSeqNP_899014.1.
UniGeneMm.316391

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteQ4KWH5.

Proteomic databases

PRIDEQ4KWH5.

Genome annotation databases

EnsemblENSMUST00000048134; ENSMUSP00000047693; ENSMUSG00000036834; Mus musculus. [Genome view]
ENSMUST00000059973; ENSMUSP00000058524; ENSMUSG00000036834; Mus musculus. [Genome view]
ENSMUST00000084105; ENSMUSP00000081122; ENSMUSG00000036834; Mus musculus. [Genome view]
GeneID269437.
KEGGmmu:269437.
UCSCuc008pju.1. mouse.
uc008pjv.1. mouse.
uc008pjw.1. mouse.
uc008pjx.1. mouse.
uc008pjy.1. mouse.
uc008pjz.1. mouse.

Organism-specific databases

CTD269437.
MGIMGI:2683547. Plch1.
RougeSearch...

Phylogenomic databases

HOGENOMQ4KWH5.
HOVERGENQ4KWH5.
OMAEFCVFYK.

Enzyme and pathway databases

BRENDA3.1.4.11. 244.

Gene expression databases

ArrayExpressQ4KWH5.
BgeeQ4KWH5.
GenevestigatorQ4KWH5.

Family and domain databases

InterProIPR000008. C2_Ca-dep.
IPR018029. C2_membr_targeting.
IPR011992. EF-Hand_type.
IPR018248. EF_hand.
IPR018247. EF_HAND_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
IPR011993. PH_type.
IPR015359. Phospholipase_C_EF-hand-like.
IPR001192. Phospholipase_C_Pinositol-sp_C.
IPR000909. Phospholipase_C_Pinositol-sp_X.
IPR001711. Phospholipase_C_Pinositol-sp_Y.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR001849. Pleckstrin_homology.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 2 hits.
G3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
PfamPF00168. C2. 1 hit.
PF00036. efhand. 1 hit.
PF09279. efhand_like. 1 hit.
PF00169. PH. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSPR00390. PHPHLIPASEC.
ProDomPD000012. EF-hand. 1 hit.
PD001202. PI_PLC_Y. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00233. PH. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
PROSITEPS50004. C2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio392850.
SOURCESearch...

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Entry information

Entry namePLCH1_MOUSE
AccessionPrimary (citable) accession number: Q4KWH5
Secondary accession number(s): Q4KWH6 expand/collapse secondary AC list , Q4KWH7, Q69ZS3, Q7TPQ1, Q8CFQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: August 2, 2005
Last modified: November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents