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Q4KMD7

- STPAP_DANRE

UniProt

Q4KMD7 - STPAP_DANRE

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Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene
tut1, rbm21, zgc:112254
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase By similarity.

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).
ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium or manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi216 – 2161Magnesium or manganese; catalytic By similarity
Metal bindingi218 – 2181Magnesium or manganese; catalytic By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3825C2H2-typeAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. mRNA 3'-UTR binding Source: UniProtKB
  4. polynucleotide adenylyltransferase activity Source: UniProtKB
  5. RNA binding Source: UniProtKB
  6. RNA uridylyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mRNA cleavage Source: UniProtKB
  2. mRNA polyadenylation Source: UniProtKB
  3. snRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
Short name:
Star-PAP
Alternative name(s):
RNA-binding motif protein 21
Short name:
RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
Short name:
U6-TUTase
Gene namesi
Name:tut1
Synonyms:rbm21
ORF Names:zgc:112254
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-050706-68. tut1.

Subcellular locationi

Nucleusnucleolus By similarity. Nucleus speckle By similarity

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB
  2. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 797797Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000404590Add
BLAST

Interactioni

Subunit structurei

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex By similarity.

Protein-protein interaction databases

STRINGi7955.ENSDARP00000074395.

Structurei

3D structure databases

ProteinModelPortaliQ4KMD7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 12673RRMAdd
BLAST
Domaini421 – 49575PAP-associatedAdd
BLAST

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3825C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5260.
HOGENOMiHOG000115998.
HOVERGENiHBG079670.
InParanoidiQ4KMD7.
PhylomeDBiQ4KMD7.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KMD7-1 [UniParc]FASTAAdd to Basket

« Hide

MELDKDIQTT QKGFHCNLCH VNIPNRPSLE DHVKGKKHLH LLRLRAQRKT    50
QEENSVFVSG FKADTSQTEL KEYFQQFGLV TDVIMDKQKG VYAIVEFSES 100
QDVQTTLAQP QHQLNGLKLR VKPREKKEFK LASRGKQDCK NTLISLDKLN 150
FELCKAMSVN EQIQKVVESL ELKDNEKKVR DLLVQLLQEV FTEFFPDCQI 200
VPFGSSVNTF GLHSCDLDLF LDLENTKVFQ ARAKSSEQTG ENQSEDCRSE 250
DSILSDIDLS TASPAEILEL VAVILRKCVP GVHKVQALST ARLPVVKFSH 300
KELNLQGDIT INNRLAVRNT KFLQLCSGID SRLRPLVYTI RLWAKQKQLA 350
GNLSGPGPLL NNYALTLLVI FFLQNRDPPV LPSVNQLKNM ACEEEECAIE 400
EWDCTFPSQP FSVPPSKNTE DLCTLLFGFF TFYSKFDFPA SVVSLRDGHV 450
LPITDFLKSD MEALKTADAS SPKPKRSSAP RLGPMNVLDP FELNHNVAGN 500
LNERTQKNFK RECCEAEKYC RSLQYQRKSA KGKSWGLVRL FAPQSEAAAS 550
SQPRAEKVLE VSVPFKPASL PESLRAQLAS AGKDFRGLWF AEVCSAVQKV 600
FNEILQCSPT EETQSLDKTD KSGSEMEVNN NRSLEDTNIQ VKGEAGKKRP 650
LSVEEGPSTF TITQAKRQRL DVDLEHPEPL HWTWTQRSRV WAGRRKVRRD 700
LLKTSDEASK PEGGCVDMES RVTQSIVEKE EKLHDALEFK VDAEVVGGNE 750
STKVVLRFHP SIDTAGVFQD FFHFLESFLP KMAETIMGRA EDITDMS 797
Length:797
Mass (Da):89,937
Last modified:August 2, 2005 - v1
Checksum:iC11F915CC7CCA221
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC098614 mRNA. Translation: AAH98614.1.
RefSeqiNP_001025359.1. NM_001030188.1.
UniGeneiDr.80967.

Genome annotation databases

GeneIDi564388.
KEGGidre:564388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC098614 mRNA. Translation: AAH98614.1 .
RefSeqi NP_001025359.1. NM_001030188.1.
UniGenei Dr.80967.

3D structure databases

ProteinModelPortali Q4KMD7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000074395.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 564388.
KEGGi dre:564388.

Organism-specific databases

CTDi 64852.
ZFINi ZDB-GENE-050706-68. tut1.

Phylogenomic databases

eggNOGi COG5260.
HOGENOMi HOG000115998.
HOVERGENi HBG079670.
InParanoidi Q4KMD7.
PhylomeDBi Q4KMD7.

Miscellaneous databases

NextBioi 20885359.
PROi Q4KMD7.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiSTPAP_DANRE
AccessioniPrimary (citable) accession number: Q4KMD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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