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Q4KMD7

- STPAP_DANRE

UniProt

Q4KMD7 - STPAP_DANRE

Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

tut1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase By similarity.By similarity

    Catalytic activityi

    UTP + RNA(n) = diphosphate + RNA(n+1).
    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi216 – 2161Magnesium or manganese; catalyticBy similarity
    Metal bindingi218 – 2181Magnesium or manganese; catalyticBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri14 – 3825C2H2-typeAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. mRNA 3'-UTR binding Source: UniProtKB
    4. polynucleotide adenylyltransferase activity Source: UniProtKB
    5. RNA binding Source: UniProtKB
    6. RNA uridylyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. mRNA cleavage Source: UniProtKB
    2. mRNA polyadenylation Source: UniProtKB
    3. snRNA processing Source: UniProtKB

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
    Short name:
    Star-PAP
    Alternative name(s):
    RNA-binding motif protein 21
    Short name:
    RNA-binding protein 21
    U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
    Short name:
    U6-TUTase
    Gene namesi
    Name:tut1
    Synonyms:rbm21
    ORF Names:zgc:112254
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-050706-68. tut1.

    Subcellular locationi

    Nucleusnucleolus By similarity. Nucleus speckle By similarity

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB
    2. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 797797Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000404590Add
    BLAST

    Interactioni

    Subunit structurei

    Associates with the cleavage and polyadenylation specificity factor (CPSF) complex.By similarity

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000074395.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4KMD7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 12673RRMPROSITE-ProRule annotationAdd
    BLAST
    Domaini421 – 49575PAP-associatedAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B-like family.Curated
    Contains 1 C2H2-type zinc finger.Curated
    Contains 1 PAP-associated domain.Curated
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri14 – 3825C2H2-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5260.
    HOGENOMiHOG000115998.
    HOVERGENiHBG079670.
    InParanoidiQ4KMD7.
    PhylomeDBiQ4KMD7.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4KMD7-1 [UniParc]FASTAAdd to Basket

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    MELDKDIQTT QKGFHCNLCH VNIPNRPSLE DHVKGKKHLH LLRLRAQRKT    50
    QEENSVFVSG FKADTSQTEL KEYFQQFGLV TDVIMDKQKG VYAIVEFSES 100
    QDVQTTLAQP QHQLNGLKLR VKPREKKEFK LASRGKQDCK NTLISLDKLN 150
    FELCKAMSVN EQIQKVVESL ELKDNEKKVR DLLVQLLQEV FTEFFPDCQI 200
    VPFGSSVNTF GLHSCDLDLF LDLENTKVFQ ARAKSSEQTG ENQSEDCRSE 250
    DSILSDIDLS TASPAEILEL VAVILRKCVP GVHKVQALST ARLPVVKFSH 300
    KELNLQGDIT INNRLAVRNT KFLQLCSGID SRLRPLVYTI RLWAKQKQLA 350
    GNLSGPGPLL NNYALTLLVI FFLQNRDPPV LPSVNQLKNM ACEEEECAIE 400
    EWDCTFPSQP FSVPPSKNTE DLCTLLFGFF TFYSKFDFPA SVVSLRDGHV 450
    LPITDFLKSD MEALKTADAS SPKPKRSSAP RLGPMNVLDP FELNHNVAGN 500
    LNERTQKNFK RECCEAEKYC RSLQYQRKSA KGKSWGLVRL FAPQSEAAAS 550
    SQPRAEKVLE VSVPFKPASL PESLRAQLAS AGKDFRGLWF AEVCSAVQKV 600
    FNEILQCSPT EETQSLDKTD KSGSEMEVNN NRSLEDTNIQ VKGEAGKKRP 650
    LSVEEGPSTF TITQAKRQRL DVDLEHPEPL HWTWTQRSRV WAGRRKVRRD 700
    LLKTSDEASK PEGGCVDMES RVTQSIVEKE EKLHDALEFK VDAEVVGGNE 750
    STKVVLRFHP SIDTAGVFQD FFHFLESFLP KMAETIMGRA EDITDMS 797
    Length:797
    Mass (Da):89,937
    Last modified:August 2, 2005 - v1
    Checksum:iC11F915CC7CCA221
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098614 mRNA. Translation: AAH98614.1.
    RefSeqiNP_001025359.1. NM_001030188.1.
    UniGeneiDr.80967.

    Genome annotation databases

    GeneIDi564388.
    KEGGidre:564388.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098614 mRNA. Translation: AAH98614.1 .
    RefSeqi NP_001025359.1. NM_001030188.1.
    UniGenei Dr.80967.

    3D structure databases

    ProteinModelPortali Q4KMD7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000074395.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 564388.
    KEGGi dre:564388.

    Organism-specific databases

    CTDi 64852.
    ZFINi ZDB-GENE-050706-68. tut1.

    Phylogenomic databases

    eggNOGi COG5260.
    HOGENOMi HOG000115998.
    HOVERGENi HBG079670.
    InParanoidi Q4KMD7.
    PhylomeDBi Q4KMD7.

    Miscellaneous databases

    NextBioi 20885359.
    PROi Q4KMD7.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR002058. PAP_assoc.
    IPR000504. RRM_dom.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiSTPAP_DANRE
    AccessioniPrimary (citable) accession number: Q4KMD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 8, 2011
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3