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Q4KMD7

- STPAP_DANRE

UniProt

Q4KMD7 - STPAP_DANRE

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Protein

Speckle targeted PIP5K1A-regulated poly(A) polymerase

Gene

tut1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase (By similarity).By similarity

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).
ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi216 – 2161Magnesium or manganese; catalyticBy similarity
Metal bindingi218 – 2181Magnesium or manganese; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3825C2H2-typeAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. mRNA 3'-UTR binding Source: UniProtKB
  4. polynucleotide adenylyltransferase activity Source: UniProtKB
  5. RNA binding Source: UniProtKB
  6. RNA uridylyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mRNA cleavage Source: UniProtKB
  2. mRNA polyadenylation Source: UniProtKB
  3. snRNA processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase (EC:2.7.7.19)
Short name:
Star-PAP
Alternative name(s):
RNA-binding motif protein 21
Short name:
RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1 (EC:2.7.7.52)
Short name:
U6-TUTase
Gene namesi
Name:tut1
Synonyms:rbm21
ORF Names:zgc:112254
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-050706-68. tut1.

Subcellular locationi

Nucleusnucleolus By similarity. Nucleus speckle By similarity

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB
  2. nucleolus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 797797Speckle targeted PIP5K1A-regulated poly(A) polymerasePRO_0000404590Add
BLAST

Interactioni

Subunit structurei

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000074395.

Structurei

3D structure databases

ProteinModelPortaliQ4KMD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 12673RRMPROSITE-ProRule annotationAdd
BLAST
Domaini421 – 49575PAP-associatedAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated
Contains 1 C2H2-type zinc finger.Curated
Contains 1 PAP-associated domain.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri14 – 3825C2H2-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5260.
HOGENOMiHOG000115998.
HOVERGENiHBG079670.
InParanoidiQ4KMD7.
PhylomeDBiQ4KMD7.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KMD7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELDKDIQTT QKGFHCNLCH VNIPNRPSLE DHVKGKKHLH LLRLRAQRKT
60 70 80 90 100
QEENSVFVSG FKADTSQTEL KEYFQQFGLV TDVIMDKQKG VYAIVEFSES
110 120 130 140 150
QDVQTTLAQP QHQLNGLKLR VKPREKKEFK LASRGKQDCK NTLISLDKLN
160 170 180 190 200
FELCKAMSVN EQIQKVVESL ELKDNEKKVR DLLVQLLQEV FTEFFPDCQI
210 220 230 240 250
VPFGSSVNTF GLHSCDLDLF LDLENTKVFQ ARAKSSEQTG ENQSEDCRSE
260 270 280 290 300
DSILSDIDLS TASPAEILEL VAVILRKCVP GVHKVQALST ARLPVVKFSH
310 320 330 340 350
KELNLQGDIT INNRLAVRNT KFLQLCSGID SRLRPLVYTI RLWAKQKQLA
360 370 380 390 400
GNLSGPGPLL NNYALTLLVI FFLQNRDPPV LPSVNQLKNM ACEEEECAIE
410 420 430 440 450
EWDCTFPSQP FSVPPSKNTE DLCTLLFGFF TFYSKFDFPA SVVSLRDGHV
460 470 480 490 500
LPITDFLKSD MEALKTADAS SPKPKRSSAP RLGPMNVLDP FELNHNVAGN
510 520 530 540 550
LNERTQKNFK RECCEAEKYC RSLQYQRKSA KGKSWGLVRL FAPQSEAAAS
560 570 580 590 600
SQPRAEKVLE VSVPFKPASL PESLRAQLAS AGKDFRGLWF AEVCSAVQKV
610 620 630 640 650
FNEILQCSPT EETQSLDKTD KSGSEMEVNN NRSLEDTNIQ VKGEAGKKRP
660 670 680 690 700
LSVEEGPSTF TITQAKRQRL DVDLEHPEPL HWTWTQRSRV WAGRRKVRRD
710 720 730 740 750
LLKTSDEASK PEGGCVDMES RVTQSIVEKE EKLHDALEFK VDAEVVGGNE
760 770 780 790
STKVVLRFHP SIDTAGVFQD FFHFLESFLP KMAETIMGRA EDITDMS
Length:797
Mass (Da):89,937
Last modified:August 2, 2005 - v1
Checksum:iC11F915CC7CCA221
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC098614 mRNA. Translation: AAH98614.1.
RefSeqiNP_001025359.1. NM_001030188.1.
UniGeneiDr.80967.

Genome annotation databases

GeneIDi564388.
KEGGidre:564388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC098614 mRNA. Translation: AAH98614.1 .
RefSeqi NP_001025359.1. NM_001030188.1.
UniGenei Dr.80967.

3D structure databases

ProteinModelPortali Q4KMD7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000074395.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 564388.
KEGGi dre:564388.

Organism-specific databases

CTDi 64852.
ZFINi ZDB-GENE-050706-68. tut1.

Phylogenomic databases

eggNOGi COG5260.
HOGENOMi HOG000115998.
HOVERGENi HBG079670.
InParanoidi Q4KMD7.
PhylomeDBi Q4KMD7.

Miscellaneous databases

NextBioi 20885359.
PROi Q4KMD7.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiSTPAP_DANRE
AccessioniPrimary (citable) accession number: Q4KMD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: August 2, 2005
Last modified: October 1, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3