Speckle targeted PIP5K1A-regulated poly(A) polymerase

Preferred order of sections

Names and origin

Protein names

Recommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase
Short name=Star-PAP
EC=2.7.7.19

Alternative name(s):
RNA-binding motif protein 21
Short name=RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1
Short name=U6-TUTase
EC=2.7.7.52

Gene names

Name:	tut1
Synonyms:	rbm21
ORF Names:	zgc:112254

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	797 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase By similarity.
Catalytic activity	UTP + RNA(n) = diphosphate + RNA(n+1). ATP + RNA(n) = diphosphate + RNA(n+1).
Cofactor	Magnesium or manganese By similarity.
Subunit structure	Associates with the cleavage and polyadenylation specificity factor (CPSF) complex By similarity.
Subcellular location	Nucleus › nucleolus By similarity. Nucleus speckle By similarity.
Sequence similarities	Belongs to the DNA polymerase type-B-like family. Contains 1 C2H2-type zinc finger. Contains 1 PAP-associated domain. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding RNA-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA cleavage Inferred from sequence or structural similarity. Source: UniProtKB mRNA polyadenylation Inferred from sequence or structural similarity. Source: UniProtKB snRNA processing Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nuclear speck Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA uridylyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB mRNA 3'-UTR binding Inferred from sequence or structural similarity. Source: UniProtKB polynucleotide adenylyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 797

797

Speckle targeted PIP5K1A-regulated poly(A) polymerase

PRO_0000404590

Regions

Domain

54 – 126

73

RRM

Domain

421 – 495

75

PAP-associated

Zinc finger

14 – 38

25

C2H2-type

Sites

Metal binding

216

1

Magnesium or manganese; catalytic By similarity

Metal binding

218

1

Magnesium or manganese; catalytic By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q4KMD7 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: C11F915CC7CCA221
Show »

FASTA

797

89,937

        10         20         30         40         50         60 
MELDKDIQTT QKGFHCNLCH VNIPNRPSLE DHVKGKKHLH LLRLRAQRKT QEENSVFVSG 

        70         80         90        100        110        120 
FKADTSQTEL KEYFQQFGLV TDVIMDKQKG VYAIVEFSES QDVQTTLAQP QHQLNGLKLR 

       130        140        150        160        170        180 
VKPREKKEFK LASRGKQDCK NTLISLDKLN FELCKAMSVN EQIQKVVESL ELKDNEKKVR 

       190        200        210        220        230        240 
DLLVQLLQEV FTEFFPDCQI VPFGSSVNTF GLHSCDLDLF LDLENTKVFQ ARAKSSEQTG 

       250        260        270        280        290        300 
ENQSEDCRSE DSILSDIDLS TASPAEILEL VAVILRKCVP GVHKVQALST ARLPVVKFSH 

       310        320        330        340        350        360 
KELNLQGDIT INNRLAVRNT KFLQLCSGID SRLRPLVYTI RLWAKQKQLA GNLSGPGPLL 

       370        380        390        400        410        420 
NNYALTLLVI FFLQNRDPPV LPSVNQLKNM ACEEEECAIE EWDCTFPSQP FSVPPSKNTE 

       430        440        450        460        470        480 
DLCTLLFGFF TFYSKFDFPA SVVSLRDGHV LPITDFLKSD MEALKTADAS SPKPKRSSAP 

       490        500        510        520        530        540 
RLGPMNVLDP FELNHNVAGN LNERTQKNFK RECCEAEKYC RSLQYQRKSA KGKSWGLVRL 

       550        560        570        580        590        600 
FAPQSEAAAS SQPRAEKVLE VSVPFKPASL PESLRAQLAS AGKDFRGLWF AEVCSAVQKV 

       610        620        630        640        650        660 
FNEILQCSPT EETQSLDKTD KSGSEMEVNN NRSLEDTNIQ VKGEAGKKRP LSVEEGPSTF 

       670        680        690        700        710        720 
TITQAKRQRL DVDLEHPEPL HWTWTQRSRV WAGRRKVRRD LLKTSDEASK PEGGCVDMES 

       730        740        750        760        770        780 
RVTQSIVEKE EKLHDALEFK VDAEVVGGNE STKVVLRFHP SIDTAGVFQD FFHFLESFLP 

       790 
KMAETIMGRA EDITDMS

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References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC098614 mRNA. Translation: AAH98614.1.
IPI	IPI00619608.
RefSeq	NP_001025359.1. NM_001030188.1.
UniGene	Dr.80967.
3D structure databases
ProteinModelPortal	Q4KMD7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	564388.
KEGG	dre:564388.
Organism-specific databases
CTD	64852.
ZFIN	ZDB-GENE-050706-68. tut1.
Phylogenomic databases
eggNOG	COG5260.
HOGENOM	HOG000115998.
HOVERGEN	HBG079670.
InParanoid	Q4KMD7.
OrthoDB	EOG480HWB.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR002058. PAP_assoc. IPR000504. RRM_dom. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF03828. PAP_assoc. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20885359.

Entry information

Entry name

STPAP_DANRE

Accession

Primary (citable) accession number: Q4KMD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	August 2, 2005
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents