Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q4KMD7 (STPAP_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Speckle targeted PIP5K1A-regulated poly(A) polymerase

Short name=Star-PAP
EC=2.7.7.19
Alternative name(s):
RNA-binding motif protein 21
Short name=RNA-binding protein 21
U6 snRNA-specific terminal uridylyltransferase 1
Short name=U6-TUTase
EC=2.7.7.52
Gene names
Name:tut1
Synonyms:rbm21
ORF Names:zgc:112254
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length797 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase By similarity.

Catalytic activity

UTP + RNA(n) = diphosphate + RNA(n+1).

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Associates with the cleavage and polyadenylation specificity factor (CPSF) complex By similarity.

Subcellular location

Nucleusnucleolus By similarity. Nucleus speckle By similarity.

Sequence similarities

Belongs to the DNA polymerase type-B-like family.

Contains 1 C2H2-type zinc finger.

Contains 1 PAP-associated domain.

Contains 1 RRM (RNA recognition motif) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 797797Speckle targeted PIP5K1A-regulated poly(A) polymerase
PRO_0000404590

Regions

Domain54 – 12673RRM
Domain421 – 49575PAP-associated
Zinc finger14 – 3825C2H2-type

Sites

Metal binding2161Magnesium or manganese; catalytic By similarity
Metal binding2181Magnesium or manganese; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4KMD7 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: C11F915CC7CCA221

FASTA79789,937
        10         20         30         40         50         60 
MELDKDIQTT QKGFHCNLCH VNIPNRPSLE DHVKGKKHLH LLRLRAQRKT QEENSVFVSG 

        70         80         90        100        110        120 
FKADTSQTEL KEYFQQFGLV TDVIMDKQKG VYAIVEFSES QDVQTTLAQP QHQLNGLKLR 

       130        140        150        160        170        180 
VKPREKKEFK LASRGKQDCK NTLISLDKLN FELCKAMSVN EQIQKVVESL ELKDNEKKVR 

       190        200        210        220        230        240 
DLLVQLLQEV FTEFFPDCQI VPFGSSVNTF GLHSCDLDLF LDLENTKVFQ ARAKSSEQTG 

       250        260        270        280        290        300 
ENQSEDCRSE DSILSDIDLS TASPAEILEL VAVILRKCVP GVHKVQALST ARLPVVKFSH 

       310        320        330        340        350        360 
KELNLQGDIT INNRLAVRNT KFLQLCSGID SRLRPLVYTI RLWAKQKQLA GNLSGPGPLL 

       370        380        390        400        410        420 
NNYALTLLVI FFLQNRDPPV LPSVNQLKNM ACEEEECAIE EWDCTFPSQP FSVPPSKNTE 

       430        440        450        460        470        480 
DLCTLLFGFF TFYSKFDFPA SVVSLRDGHV LPITDFLKSD MEALKTADAS SPKPKRSSAP 

       490        500        510        520        530        540 
RLGPMNVLDP FELNHNVAGN LNERTQKNFK RECCEAEKYC RSLQYQRKSA KGKSWGLVRL 

       550        560        570        580        590        600 
FAPQSEAAAS SQPRAEKVLE VSVPFKPASL PESLRAQLAS AGKDFRGLWF AEVCSAVQKV 

       610        620        630        640        650        660 
FNEILQCSPT EETQSLDKTD KSGSEMEVNN NRSLEDTNIQ VKGEAGKKRP LSVEEGPSTF 

       670        680        690        700        710        720 
TITQAKRQRL DVDLEHPEPL HWTWTQRSRV WAGRRKVRRD LLKTSDEASK PEGGCVDMES 

       730        740        750        760        770        780 
RVTQSIVEKE EKLHDALEFK VDAEVVGGNE STKVVLRFHP SIDTAGVFQD FFHFLESFLP 

       790 
KMAETIMGRA EDITDMS 

« Hide

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC098614 mRNA. Translation: AAH98614.1.
RefSeqNP_001025359.1. NM_001030188.1.
UniGeneDr.80967.

3D structure databases

ProteinModelPortalQ4KMD7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000074395.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID564388.
KEGGdre:564388.

Organism-specific databases

CTD64852.
ZFINZDB-GENE-050706-68. tut1.

Phylogenomic databases

eggNOGCOG5260.
HOGENOMHOG000115998.
HOVERGENHBG079670.
InParanoidQ4KMD7.
PhylomeDBQ4KMD7.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR002058. PAP_assoc.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamPF03828. PAP_assoc. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20885359.
PROQ4KMD7.

Entry information

Entry nameSTPAP_DANRE
AccessionPrimary (citable) accession number: Q4KMD7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families