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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2

Gene

Gfpt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-KW
  3. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_287410. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKQ4KMC4.
UniPathwayiUPA00113; UER00528.

Protein family/group databases

MEROPSiC44.975.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 2
Glutamine:fructose-6-phosphate amidotransferase 2
Short name:
GFAT 2
Short name:
GFAT2
Hexosephosphate aminotransferase 2
Gene namesi
Name:Gfpt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1303097. Gfpt2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 682681Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2PRO_0000135285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei244 – 2441PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4KMC4.
PRIDEiQ4KMC4.

Expressioni

Gene expression databases

GenevestigatoriQ4KMC4.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003770.

Structurei

3D structure databases

ProteinModelPortaliQ4KMC4.
SMRiQ4KMC4. Positions 322-670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 288287Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini360 – 499140SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini531 – 672142SIS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiQ4KMC4.
KOiK00820.
OMAiDCLEVFK.
OrthoDBiEOG73NG2S.
PhylomeDBiQ4KMC4.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4KMC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFAYMNY RVPKTRKEIF ETLIRGLQRL EYRGYDSAGV AIDGNNHEVK
60 70 80 90 100
ERHIHLVKKK GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPN
110 120 130 140 150
VVNSHPQRSD KDNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA
160 170 180 190 200
KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSIH YPGEAVATRR
210 220 230 240 250
GSPLLIGVRS KYKLSTEQIP VLYRTCTIEN VKNICKTRLK RLDSSTCLHA
260 270 280 290 300
VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAS
310 320 330 340 350
DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN
360 370 380 390 400
TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE
410 420 430 440 450
LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGITNT
460 470 480 490 500
VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL
510 520 530 540 550
QTRRQEIIRG LRSLPELIKE VLSLDEKIHD LALELYTQRS LLVMGRGYNY
560 570 580 590 600
ATCLEGALKI KEITYMHSEG ILAGELKHGP LALVDKQMPV IMVIMKDPCF
610 620 630 640 650
AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS
660 670 680
VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE
Length:682
Mass (Da):77,010
Last modified:January 23, 2007 - v3
Checksum:iE4B4B656999F667A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098630 mRNA. Translation: AAH98630.1.
RefSeqiNP_001002819.2. NM_001002819.2.
UniGeneiRn.16089.

Genome annotation databases

EnsembliENSRNOT00000003770; ENSRNOP00000003770; ENSRNOG00000002810.
GeneIDi360518.
KEGGirno:360518.
UCSCiRGD:1303097. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098630 mRNA. Translation: AAH98630.1.
RefSeqiNP_001002819.2. NM_001002819.2.
UniGeneiRn.16089.

3D structure databases

ProteinModelPortaliQ4KMC4.
SMRiQ4KMC4. Positions 322-670.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003770.

Protein family/group databases

MEROPSiC44.975.

Proteomic databases

PaxDbiQ4KMC4.
PRIDEiQ4KMC4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003770; ENSRNOP00000003770; ENSRNOG00000002810.
GeneIDi360518.
KEGGirno:360518.
UCSCiRGD:1303097. rat.

Organism-specific databases

CTDi9945.
RGDi1303097. Gfpt2.

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiQ4KMC4.
KOiK00820.
OMAiDCLEVFK.
OrthoDBiEOG73NG2S.
PhylomeDBiQ4KMC4.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.
ReactomeiREACT_287410. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKQ4KMC4.

Miscellaneous databases

NextBioi673062.
PROiQ4KMC4.

Gene expression databases

GenevestigatoriQ4KMC4.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiGFPT2_RAT
AccessioniPrimary (citable) accession number: Q4KMC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.