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Protein

tRNA pseudouridine synthase A

Gene

Pus1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts specific uridines to PSI in a number of tRNA substrates. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 RNA.By similarity

Catalytic activityi

tRNA uridine(38-40) = tRNA pseudouridine(38-40).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei142NucleophileBy similarity1
Binding sitei197SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase A (EC:5.4.99.12)
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene namesi
Name:Pus1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1311871 Pus1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000248245? – 423tRNA pseudouridine synthase A
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei411PhosphoserineBy similarity1
Modified residuei416PhosphoserineCombined sources1
Modified residuei422PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4KM92
PRIDEiQ4KM92

PTM databases

iPTMnetiQ4KM92
PhosphoSitePlusiQ4KM92

Expressioni

Gene expression databases

BgeeiENSRNOG00000037500
ExpressionAtlasiQ4KM92 baseline and differential
GenevisibleiQ4KM92 RN

Interactioni

Subunit structurei

Monomer (By similarity). Forms a complex with RARG and the SRA1 RNA in the nucleus (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000053623

Structurei

3D structure databases

ProteinModelPortaliQ4KM92
SMRiQ4KM92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2553 Eukaryota
COG0101 LUCA
GeneTreeiENSGT00510000047706
HOGENOMiHOG000031229
HOVERGENiHBG000489
InParanoidiQ4KM92
KOiK06173
OMAiKNKCDAR
OrthoDBiEOG091G0CIE
PhylomeDBiQ4KM92

Family and domain databases

Gene3Di3.30.70.660, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001406 PsdUridine_synth_TruA
IPR020097 PsdUridine_synth_TruA_a/b_dom
IPR020095 PsdUridine_synth_TruA_C
PANTHERiPTHR11142 PTHR11142, 1 hit
PfamiView protein in Pfam
PF01416 PseudoU_synth_1, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00071 hisT_truA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4KM92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFPRLWAAL LRAWGRWTAR PGPRVPGLPP MAGNKVPPAL PSHQPDRKGR
60 70 80 90 100
GGWVWEETEH PAKRVKGGED EEPPRKLPKR KIVLLMAYSG KGYHGMQRNL
110 120 130 140 150
GSSQFRTIED DLVSALVQAG CIPENHGTDM RKMSFQRCAR TDKGVSAAGQ
160 170 180 190 200
VVSLKVWLID DILDKINSHL PSHIRILGLK RVTGGFNSKN KCDARTYCYM
210 220 230 240 250
LPTFAFAHKD RDVQDESYRL SAETLQQVNR LLGCYKGTHN FHNFTSQKGP
260 270 280 290 300
REPSARRYIL EMYCEEPFVR EGLEFAVIKV KGQSFMMHQI RKMVGLVVAI
310 320 330 340 350
VKGYAPESVL ERSWGEEKVD VPKAPGLGLV LERVHFEKYN QRFGSDGLHE
360 370 380 390 400
PLDWAQEEGK VTAFKEQYIY PTIVSTERDE RSMAQWLNTL PIHNFSGTAL
410 420
GADDTGAKVP SSLEGSEGDG DTD
Length:423
Mass (Da):47,515
Last modified:April 8, 2008 - v2
Checksum:iBCF4780D9D439520
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC095390 Genomic DNA No translation available.
RefSeqiNP_001020734.1, NM_001025563.1
UniGeneiRn.11475

Genome annotation databases

EnsembliENSRNOT00000056780; ENSRNOP00000053623; ENSRNOG00000037500
GeneIDi304567
KEGGirno:304567
UCSCiRGD:1311871 rat

Similar proteinsi

Entry informationi

Entry nameiTRUA_RAT
AccessioniPrimary (citable) accession number: Q4KM92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: April 8, 2008
Last modified: May 23, 2018
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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