Reviewed, UniProtKB/Swiss-Prot Q4KM74 (SC22B_RAT)

Names and origin

Protein names

Recommended name:
    Vesicle-trafficking protein SEC22b
Alternative name(s):
    SEC22 vesicle-trafficking protein homolog B
    SEC22 vesicle-trafficking protein-like 1
    ERS24
    ERS-24

Gene names

Name:	Sec22b
Synonyms:	Sec22l1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER. Ref.2 UniProtKB O75396
Subunit structure	Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B as subunit of either complex. Ref.2 UniProtKB O75396
Subcellular location	Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein Potential. Melanosome By similarity Ref.3 UniProtKB O75396.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Coiled coil Transmembrane
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity UniProtKB O75396
	Chain	2 – 215	214	Vesicle-trafficking protein SEC22b		PRO_0000253048
Regions
	Topological domain	2 – 194	193	Cytoplasmic Potential
	Transmembrane	195 – 215	21	Anchor for type IV membrane protein Potential
	Domain	6 – 119	114	Longin
	Domain	134 – 194	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	35	1	Phosphoserine By similarity
	Modified residue	38	1	N6-acetyllysine By similarity
	Modified residue	48	1	Phosphoserine By similarity
	Modified residue	137	1	Phosphoserine By similarity
	Modified residue	168	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KM74-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 29B4C55961C5A044	FASTA	215	24,741
	10 20 30 40 50 60 MVLLTMIARV ADGLPLAASM QEDEQSGRDL QQYQSQAKQL FRKLNEQSPT RCTLEAGAMT 70 80 90 100 110 120 FHYIIEQGVC YLVLCEAAFP KKLAFAYLED LHSEFDEQHG KKVPTVSRPY SFIEFDTFIQ 130 140 150 160 170 180 KTKKLYIDSR ARRNLGSINT ELQDVQRIMV ANIEEVLQRG EALSALDSKA NNLSSLSKKY 190 200 210 RQDAKYLNMR STYAKLAAVA VFFIMLIVYV RFWWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[2]	"Subunit structure of a mammalian ER/Golgi SNARE complex." Xu D., Joglekar A.P., Williams A.L., Hay J.C. J. Biol. Chem. 275:39631-39639(2000) [PubMed: 11035026] [Abstract] Cited for: FUNCTION, INTERACTION WITH BET1; GOSR2 AND STX5.
[3]	"Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack." Volchuk A., Ravazzola M., Perrelet A., Eng W.S., Di Liberto M., Varlamov O., Fukasawa M., Engel T., Sollner T.H., Rothman J.E., Orci L. Mol. Biol. Cell 15:1506-1518(2004) [PubMed: 14742712] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC098725 mRNA. Translation: AAH98725.1.
IPI	IPI00193522.
RefSeq	NP_001020857.1.
UniGene	Rn.102384
3D structure databases
HSSP	HSSP built from PDB template 1IFQ based on UniProtKB O08547.
SMR	Q4KM74. Positions 1-157, 135-185.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KM74.
Proteomic databases
PRIDE	Q4KM74.
Genome annotation databases
Ensembl	ENSRNOT00000025327; ENSRNOP00000025327; ENSRNOG00000018673; Rattus norvegicus. [Genome view]
GeneID	310710.
KEGG	rno:310710.
UCSC	NM_001025686. rat.
Organism-specific databases
CTD	310710.
RGD	1309988. Sec22b.
Phylogenomic databases
eggNOG	maNOG18150.
HOVERGEN	Q4KM74.
InParanoid	Q4KM74.
OMA	NQAKMLF.
PhylomeDB	Q4KM74.
Gene expression databases
ArrayExpress	Q4KM74.
Genevestigator	Q4KM74.
GermOnline	ENSRNOG00000018673. Rattus norvegicus.
Family and domain databases
InterPro	IPR010908. Longin. IPR011012. Longin-like. IPR001388. Synaptobrevin. [Graphical view]
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PRINTS	PR00219. SYNAPTOBREVN.
PROSITE	PS50859. LONGIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	662501.

Entry information

Entry name

SC22B_RAT

Accession

Primary (citable) accession number: Q4KM74

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 42 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)