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Protein

B-cell linker protein

Gene

Blnk

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

B-cell activation

Enzyme and pathway databases

ReactomeiREACT_320606. Regulation of signaling by CBL.
REACT_341785. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Names & Taxonomyi

Protein namesi
Recommended name:
B-cell linker protein
Alternative name(s):
Cytoplasmic adapter protein
Gene namesi
Name:Blnk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1561933. Blnk.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: BCR activation results in the translocation to membrane fraction.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457B-cell linker proteinPRO_0000064942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei72 – 721Phosphotyrosine; by SYKBy similarity
Modified residuei84 – 841Phosphotyrosine; by SYKBy similarity
Modified residuei96 – 961Phosphotyrosine; by SYKBy similarity
Modified residuei178 – 1781Phosphotyrosine; by SYKBy similarity
Modified residuei189 – 1891Phosphotyrosine; by SYKBy similarity

Post-translational modificationi

Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca2+ and MAPK signaling pathways (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4KM52.
PRIDEiQ4KM52.

PTM databases

PhosphoSiteiQ4KM52.

Expressioni

Gene expression databases

GenevisibleiQ4KM52. RN.

Interactioni

Subunit structurei

Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with VAV3, PLCG2 and GRB2. Interacts through its SH2 domain with CD79A. Interacts (via SH2 domain) with SYK; phosphorylated and activated by SYK. Interacts with SCIMP (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019014.

Structurei

3D structure databases

ProteinModelPortaliQ4KM52.
SMRiQ4KM52. Positions 328-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini347 – 454108SH2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi130 – 330201Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiNOG44661.
GeneTreeiENSGT00530000063094.
HOGENOMiHOG000088646.
HOVERGENiHBG053147.
InParanoidiQ4KM52.
KOiK07371.
OMAiHRQENMQ.
OrthoDBiEOG7WMCKM.
PhylomeDBiQ4KM52.
TreeFamiTF326567.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
[Graphical view]
SMARTiSM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KM52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMDKIKKLK VKGPPSVPRR
60 70 80 90 100
DYALDNPADE EEQWSDDFDS DYENPDEHSD SEMYVMPAEE TGDDSYEPPP
110 120 130 140 150
AEQQTRVVHP ALPFTRGEYV DNRSSQRHSP PFSKTLPSKP SWPSAKARLA
160 170 180 190 200
STLPAPNSLQ KPQVPPKPKD LLEDEADYVV PVEDNDENYI HPRESSPLPA
210 220 230 240 250
EKAPTVNRST KPNSSSKHVS PPGTVAGRNS GVWDSKSSLP AAPSPLPRAG
260 270 280 290 300
KKTATPLKTT PVPSLQNASN VCEEKPVPAE RHRGSSHRQD TVQSPVFPPT
310 320 330 340 350
QKPVLQKPVP LPRFTEGGSP AADGPVPSFP FNSTFADQEA ELHGKPWYAG
360 370 380 390 400
ACDRKSAEEA LHRSNKDGSF LIRKSSGHDS KQPYTLVAFF NKRVYNIPVR
410 420 430 440 450
FIEATKQYAL GKKKNGEEYF GSVVEIIKNH QHNPLVLIDS QNNTKDSTRL

KYAVKVS
Length:457
Mass (Da):50,634
Last modified:August 2, 2005 - v1
Checksum:iA40DA57A13022483
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098790 mRNA. Translation: AAH98790.1.
RefSeqiNP_001020938.1. NM_001025767.1.
UniGeneiRn.32684.

Genome annotation databases

EnsembliENSRNOT00000019014; ENSRNOP00000019014; ENSRNOG00000013967.
GeneIDi499356.
KEGGirno:499356.
UCSCiRGD:1561933. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098790 mRNA. Translation: AAH98790.1.
RefSeqiNP_001020938.1. NM_001025767.1.
UniGeneiRn.32684.

3D structure databases

ProteinModelPortaliQ4KM52.
SMRiQ4KM52. Positions 328-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019014.

PTM databases

PhosphoSiteiQ4KM52.

Proteomic databases

PaxDbiQ4KM52.
PRIDEiQ4KM52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019014; ENSRNOP00000019014; ENSRNOG00000013967.
GeneIDi499356.
KEGGirno:499356.
UCSCiRGD:1561933. rat.

Organism-specific databases

CTDi29760.
RGDi1561933. Blnk.

Phylogenomic databases

eggNOGiNOG44661.
GeneTreeiENSGT00530000063094.
HOGENOMiHOG000088646.
HOVERGENiHBG053147.
InParanoidiQ4KM52.
KOiK07371.
OMAiHRQENMQ.
OrthoDBiEOG7WMCKM.
PhylomeDBiQ4KM52.
TreeFamiTF326567.

Enzyme and pathway databases

ReactomeiREACT_320606. Regulation of signaling by CBL.
REACT_341785. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Miscellaneous databases

NextBioi702642.
PROiQ4KM52.

Gene expression databases

GenevisibleiQ4KM52. RN.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
[Graphical view]
SMARTiSM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.

Entry informationi

Entry nameiBLNK_RAT
AccessioniPrimary (citable) accession number: Q4KM52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: August 2, 2005
Last modified: June 24, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.