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Q4KM52

- BLNK_RAT

UniProt

Q4KM52 - BLNK_RAT

Protein

B-cell linker protein

Gene

Blnk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis By similarity.By similarity

    GO - Biological processi

    1. B cell activation Source: UniProtKB-KW
    2. intracellular signal transduction Source: Ensembl

    Keywords - Biological processi

    B-cell activation

    Enzyme and pathway databases

    ReactomeiREACT_196225. Regulation of signaling by CBL.
    REACT_211910. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    B-cell linker protein
    Alternative name(s):
    Cytoplasmic adapter protein
    Gene namesi
    Name:Blnk
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi1561933. Blnk.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: BCR activation results in the translocation to membrane fraction.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 457457B-cell linker proteinPRO_0000064942Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei72 – 721Phosphotyrosine; by SYKBy similarity
    Modified residuei84 – 841Phosphotyrosine; by SYKBy similarity
    Modified residuei96 – 961Phosphotyrosine; by SYKBy similarity
    Modified residuei178 – 1781Phosphotyrosine; by SYKBy similarity
    Modified residuei189 – 1891Phosphotyrosine; by SYKBy similarity

    Post-translational modificationi

    Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca2+ and MAPK signaling pathways By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ4KM52.
    PRIDEiQ4KM52.

    PTM databases

    PhosphoSiteiQ4KM52.

    Expressioni

    Gene expression databases

    GenevestigatoriQ4KM52.

    Interactioni

    Subunit structurei

    Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with VAV3, PLCG2 and GRB2. Interacts through its SH2 domain with CD79A. Interacts (via SH2 domain) with SYK; phosphorylated and activated by SYK. Interacts with SCIMP By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000019014.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4KM52.
    SMRiQ4KM52. Positions 328-457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini347 – 454108SH2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi130 – 330201Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain

    Phylogenomic databases

    eggNOGiNOG44661.
    GeneTreeiENSGT00530000063094.
    HOGENOMiHOG000088646.
    HOVERGENiHBG053147.
    KOiK07371.
    OMAiHRQENMQ.
    OrthoDBiEOG7WMCKM.
    PhylomeDBiQ4KM52.
    TreeFamiTF326567.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR000980. SH2.
    [Graphical view]
    PfamiPF00017. SH2. 1 hit.
    [Graphical view]
    SMARTiSM00252. SH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55550. SSF55550. 1 hit.
    PROSITEiPS50001. SH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4KM52-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMDKIKKLK VKGPPSVPRR    50
    DYALDNPADE EEQWSDDFDS DYENPDEHSD SEMYVMPAEE TGDDSYEPPP 100
    AEQQTRVVHP ALPFTRGEYV DNRSSQRHSP PFSKTLPSKP SWPSAKARLA 150
    STLPAPNSLQ KPQVPPKPKD LLEDEADYVV PVEDNDENYI HPRESSPLPA 200
    EKAPTVNRST KPNSSSKHVS PPGTVAGRNS GVWDSKSSLP AAPSPLPRAG 250
    KKTATPLKTT PVPSLQNASN VCEEKPVPAE RHRGSSHRQD TVQSPVFPPT 300
    QKPVLQKPVP LPRFTEGGSP AADGPVPSFP FNSTFADQEA ELHGKPWYAG 350
    ACDRKSAEEA LHRSNKDGSF LIRKSSGHDS KQPYTLVAFF NKRVYNIPVR 400
    FIEATKQYAL GKKKNGEEYF GSVVEIIKNH QHNPLVLIDS QNNTKDSTRL 450
    KYAVKVS 457
    Length:457
    Mass (Da):50,634
    Last modified:August 2, 2005 - v1
    Checksum:iA40DA57A13022483
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098790 mRNA. Translation: AAH98790.1.
    RefSeqiNP_001020938.1. NM_001025767.1.
    UniGeneiRn.32684.

    Genome annotation databases

    EnsembliENSRNOT00000019014; ENSRNOP00000019014; ENSRNOG00000013967.
    GeneIDi499356.
    KEGGirno:499356.
    UCSCiRGD:1561933. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098790 mRNA. Translation: AAH98790.1 .
    RefSeqi NP_001020938.1. NM_001025767.1.
    UniGenei Rn.32684.

    3D structure databases

    ProteinModelPortali Q4KM52.
    SMRi Q4KM52. Positions 328-457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000019014.

    PTM databases

    PhosphoSitei Q4KM52.

    Proteomic databases

    PaxDbi Q4KM52.
    PRIDEi Q4KM52.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000019014 ; ENSRNOP00000019014 ; ENSRNOG00000013967 .
    GeneIDi 499356.
    KEGGi rno:499356.
    UCSCi RGD:1561933. rat.

    Organism-specific databases

    CTDi 29760.
    RGDi 1561933. Blnk.

    Phylogenomic databases

    eggNOGi NOG44661.
    GeneTreei ENSGT00530000063094.
    HOGENOMi HOG000088646.
    HOVERGENi HBG053147.
    KOi K07371.
    OMAi HRQENMQ.
    OrthoDBi EOG7WMCKM.
    PhylomeDBi Q4KM52.
    TreeFami TF326567.

    Enzyme and pathway databases

    Reactomei REACT_196225. Regulation of signaling by CBL.
    REACT_211910. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

    Miscellaneous databases

    NextBioi 702642.
    PROi Q4KM52.

    Gene expression databases

    Genevestigatori Q4KM52.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR000980. SH2.
    [Graphical view ]
    Pfami PF00017. SH2. 1 hit.
    [Graphical view ]
    SMARTi SM00252. SH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55550. SSF55550. 1 hit.
    PROSITEi PS50001. SH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Spleen.

    Entry informationi

    Entry nameiBLNK_RAT
    AccessioniPrimary (citable) accession number: Q4KM52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3