Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q4KM52

- BLNK_RAT

UniProt

Q4KM52 - BLNK_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

B-cell linker protein

Gene

Blnk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis By similarity.By similarity

GO - Biological processi

  1. B cell activation Source: UniProtKB-KW
  2. intracellular signal transduction Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

B-cell activation

Enzyme and pathway databases

ReactomeiREACT_196225. Regulation of signaling by CBL.
REACT_211910. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Names & Taxonomyi

Protein namesi
Recommended name:
B-cell linker protein
Alternative name(s):
Cytoplasmic adapter protein
Gene namesi
Name:Blnk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi1561933. Blnk.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: BCR activation results in the translocation to membrane fraction.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457B-cell linker proteinPRO_0000064942Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei72 – 721Phosphotyrosine; by SYKBy similarity
Modified residuei84 – 841Phosphotyrosine; by SYKBy similarity
Modified residuei96 – 961Phosphotyrosine; by SYKBy similarity
Modified residuei178 – 1781Phosphotyrosine; by SYKBy similarity
Modified residuei189 – 1891Phosphotyrosine; by SYKBy similarity

Post-translational modificationi

Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca2+ and MAPK signaling pathways By similarity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4KM52.
PRIDEiQ4KM52.

PTM databases

PhosphoSiteiQ4KM52.

Expressioni

Gene expression databases

GenevestigatoriQ4KM52.

Interactioni

Subunit structurei

Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with VAV3, PLCG2 and GRB2. Interacts through its SH2 domain with CD79A. Interacts (via SH2 domain) with SYK; phosphorylated and activated by SYK. Interacts with SCIMP By similarity.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019014.

Structurei

3D structure databases

ProteinModelPortaliQ4KM52.
SMRiQ4KM52. Positions 328-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini347 – 454108SH2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi130 – 330201Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiNOG44661.
GeneTreeiENSGT00530000063094.
HOGENOMiHOG000088646.
HOVERGENiHBG053147.
InParanoidiQ4KM52.
KOiK07371.
OMAiHRQENMQ.
OrthoDBiEOG7WMCKM.
PhylomeDBiQ4KM52.
TreeFamiTF326567.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
[Graphical view]
SMARTiSM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KM52-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMDKIKKLK VKGPPSVPRR
60 70 80 90 100
DYALDNPADE EEQWSDDFDS DYENPDEHSD SEMYVMPAEE TGDDSYEPPP
110 120 130 140 150
AEQQTRVVHP ALPFTRGEYV DNRSSQRHSP PFSKTLPSKP SWPSAKARLA
160 170 180 190 200
STLPAPNSLQ KPQVPPKPKD LLEDEADYVV PVEDNDENYI HPRESSPLPA
210 220 230 240 250
EKAPTVNRST KPNSSSKHVS PPGTVAGRNS GVWDSKSSLP AAPSPLPRAG
260 270 280 290 300
KKTATPLKTT PVPSLQNASN VCEEKPVPAE RHRGSSHRQD TVQSPVFPPT
310 320 330 340 350
QKPVLQKPVP LPRFTEGGSP AADGPVPSFP FNSTFADQEA ELHGKPWYAG
360 370 380 390 400
ACDRKSAEEA LHRSNKDGSF LIRKSSGHDS KQPYTLVAFF NKRVYNIPVR
410 420 430 440 450
FIEATKQYAL GKKKNGEEYF GSVVEIIKNH QHNPLVLIDS QNNTKDSTRL

KYAVKVS
Length:457
Mass (Da):50,634
Last modified:August 2, 2005 - v1
Checksum:iA40DA57A13022483
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC098790 mRNA. Translation: AAH98790.1.
RefSeqiNP_001020938.1. NM_001025767.1.
UniGeneiRn.32684.

Genome annotation databases

EnsembliENSRNOT00000019014; ENSRNOP00000019014; ENSRNOG00000013967.
GeneIDi499356.
KEGGirno:499356.
UCSCiRGD:1561933. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC098790 mRNA. Translation: AAH98790.1 .
RefSeqi NP_001020938.1. NM_001025767.1.
UniGenei Rn.32684.

3D structure databases

ProteinModelPortali Q4KM52.
SMRi Q4KM52. Positions 328-457.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000019014.

PTM databases

PhosphoSitei Q4KM52.

Proteomic databases

PaxDbi Q4KM52.
PRIDEi Q4KM52.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000019014 ; ENSRNOP00000019014 ; ENSRNOG00000013967 .
GeneIDi 499356.
KEGGi rno:499356.
UCSCi RGD:1561933. rat.

Organism-specific databases

CTDi 29760.
RGDi 1561933. Blnk.

Phylogenomic databases

eggNOGi NOG44661.
GeneTreei ENSGT00530000063094.
HOGENOMi HOG000088646.
HOVERGENi HBG053147.
InParanoidi Q4KM52.
KOi K07371.
OMAi HRQENMQ.
OrthoDBi EOG7WMCKM.
PhylomeDBi Q4KM52.
TreeFami TF326567.

Enzyme and pathway databases

Reactomei REACT_196225. Regulation of signaling by CBL.
REACT_211910. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Miscellaneous databases

NextBioi 702642.
PROi Q4KM52.

Gene expression databases

Genevestigatori Q4KM52.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR000980. SH2.
[Graphical view ]
Pfami PF00017. SH2. 1 hit.
[Graphical view ]
SMARTi SM00252. SH2. 1 hit.
[Graphical view ]
SUPFAMi SSF55550. SSF55550. 1 hit.
PROSITEi PS50001. SH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.

Entry informationi

Entry nameiBLNK_RAT
AccessioniPrimary (citable) accession number: Q4KM52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: August 2, 2005
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3