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Q4KM52 (BLNK_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
B-cell linker protein
Alternative name(s):
Cytoplasmic adapter protein
Gene names
Name:Blnk
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis By similarity.

Subunit structure

Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with VAV3, PLCG2 and GRB2. Interacts through its SH2 domain with CD79A. Interacts (via SH2 domain) with SYK; phosphorylated and activated by SYK. Interacts with SCIMP By similarity.

Subcellular location

Cytoplasm By similarity. Cell membrane By similarity. Note: BCR activation results in the translocation to membrane fraction By similarity.

Post-translational modification

Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca2+ and MAPK signaling pathways By similarity.

Sequence similarities

Contains 1 SH2 domain.

Ontologies

Keywords
   Biological processB-cell activation
   Cellular componentCell membrane
Cytoplasm
Membrane
   DomainSH2 domain
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell activation

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular signal transduction

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457B-cell linker protein
PRO_0000064942

Regions

Domain347 – 454108SH2
Compositional bias130 – 330201Pro-rich

Amino acid modifications

Modified residue721Phosphotyrosine; by SYK By similarity
Modified residue841Phosphotyrosine; by SYK By similarity
Modified residue961Phosphotyrosine; by SYK By similarity
Modified residue1781Phosphotyrosine; by SYK By similarity
Modified residue1891Phosphotyrosine; by SYK By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4KM52 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: A40DA57A13022483

FASTA45750,634
        10         20         30         40         50         60 
MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMDKIKKLK VKGPPSVPRR DYALDNPADE 

        70         80         90        100        110        120 
EEQWSDDFDS DYENPDEHSD SEMYVMPAEE TGDDSYEPPP AEQQTRVVHP ALPFTRGEYV 

       130        140        150        160        170        180 
DNRSSQRHSP PFSKTLPSKP SWPSAKARLA STLPAPNSLQ KPQVPPKPKD LLEDEADYVV 

       190        200        210        220        230        240 
PVEDNDENYI HPRESSPLPA EKAPTVNRST KPNSSSKHVS PPGTVAGRNS GVWDSKSSLP 

       250        260        270        280        290        300 
AAPSPLPRAG KKTATPLKTT PVPSLQNASN VCEEKPVPAE RHRGSSHRQD TVQSPVFPPT 

       310        320        330        340        350        360 
QKPVLQKPVP LPRFTEGGSP AADGPVPSFP FNSTFADQEA ELHGKPWYAG ACDRKSAEEA 

       370        380        390        400        410        420 
LHRSNKDGSF LIRKSSGHDS KQPYTLVAFF NKRVYNIPVR FIEATKQYAL GKKKNGEEYF 

       430        440        450 
GSVVEIIKNH QHNPLVLIDS QNNTKDSTRL KYAVKVS 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC098790 mRNA. Translation: AAH98790.1.
RefSeqNP_001020938.1. NM_001025767.1.
UniGeneRn.32684.

3D structure databases

ProteinModelPortalQ4KM52.
SMRQ4KM52. Positions 328-457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000019014.

PTM databases

PhosphoSiteQ4KM52.

Proteomic databases

PaxDbQ4KM52.
PRIDEQ4KM52.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000019014; ENSRNOP00000019014; ENSRNOG00000013967.
GeneID499356.
KEGGrno:499356.
UCSCRGD:1561933. rat.

Organism-specific databases

CTD29760.
RGD1561933. Blnk.

Phylogenomic databases

eggNOGNOG44661.
GeneTreeENSGT00530000063094.
HOGENOMHOG000088646.
HOVERGENHBG053147.
KOK07371.
OMAHRQENMQ.
OrthoDBEOG7WMCKM.
PhylomeDBQ4KM52.
TreeFamTF326567.

Gene expression databases

GenevestigatorQ4KM52.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR000980. SH2.
[Graphical view]
PfamPF00017. SH2. 1 hit.
[Graphical view]
SMARTSM00252. SH2. 1 hit.
[Graphical view]
SUPFAMSSF55550. SSF55550. 1 hit.
PROSITEPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio702642.
PROQ4KM52.

Entry information

Entry nameBLNK_RAT
AccessionPrimary (citable) accession number: Q4KM52
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families