ID   SYYC_RAT                Reviewed;         528 AA.
AC   Q4KM49;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 31.
DE   RecName: Full=Tyrosyl-tRNA synthetase, cytoplasmic;
DE            EC=6.1.1.1;
DE   AltName: Full=Tyrosyl--tRNA ligase;
DE            Short=TyrRS;
GN   Name=Yars;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a
CC       two-step reaction: tyrosine is first activated by ATP to form Tyr-
CC       AMP and then transferred to the acceptor end of tRNA(Tyr) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + L-tyrosine + tRNA(Tyr) = AMP +
CC       diphosphate + L-tyrosyl-tRNA(Tyr).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
CC   -!- SIMILARITY: Contains 1 tRNA-binding domain.
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DR   EMBL; BC098795; AAH98795.1; -; mRNA.
DR   IPI; IPI00366785; -.
DR   RefSeq; NP_001020867.1; -.
DR   UniGene; Rn.41002; -.
DR   SMR; Q4KM49; 3-341, 4-342, 359-527, 360-528.
DR   PhosphoSite; Q4KM49; -.
DR   Ensembl; ENSRNOG00000007213; Rattus norvegicus.
DR   GeneID; 313047; -.
DR   KEGG; rno:313047; -.
DR   NMPDR; fig|10116.3.peg.23990; -.
DR   RGD; 1307616; Yars.
DR   HOVERGEN; Q4KM49; -.
DR   OMA; Q4KM49; LCASIEG.
DR   BRENDA; 6.1.1.1; 248.
DR   NextBio; 665526; -.
DR   ArrayExpress; Q4KM49; -.
DR   GermOnline; ENSRNOG00000007213; Rattus norvegicus.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004831; F:tyrosine-tRNA ligase activity; IEA:EC.
DR   GO; GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR002305; aa-tRNA-synth_Ib.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR002547; tRNA_bd.
DR   InterPro; IPR015624; Tyr-tRNA-synth_Ib_arc/euk.
DR   InterPro; IPR002307; Tyr-tRNA-synth_Ib_bac/mito.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR11946:SF8; Tyr-tRNA_synth; 1.
DR   Pfam; PF00579; tRNA-synt_1b; 1.
DR   Pfam; PF01588; tRNA_bind; 1.
DR   PRINTS; PR01040; TRNASYNTHTYR.
DR   TIGRFAMs; TIGR00234; tyrS; 1.
DR   PROSITE; PS50886; TRBD; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm;
KW   Ligase; Nucleotide-binding; Protein biosynthesis; RNA-binding;
KW   tRNA-binding.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    528       Tyrosyl-tRNA synthetase, cytoplasmic.
FT                                /FTId=PRO_0000239691.
FT   DOMAIN      364    468       tRNA-binding.
FT   MOTIF        44     52       "HIGH" region (By similarity).
FT   MOTIF       222    226       "KMSKS" region (By similarity).
FT   BINDING      39     39       Tyrosine (By similarity).
FT   BINDING     166    166       Tyrosine (By similarity).
FT   BINDING     170    170       Tyrosine (By similarity).
FT   BINDING     173    173       Tyrosine (By similarity).
FT   BINDING     188    188       Tyrosine (By similarity).
FT   MOD_RES       2      2       N-acetylglycine (By similarity).
SQ   SEQUENCE   528 AA;  59115 MW;  AD79FC551BEC9CBA CRC64;
     MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKVYW GTATTGKPHV AYFVPMSKIA
     DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRTSYYENV IKAMLESIGV PLEKLKFTKG
     TDYQLSKEYT LDVYRLSSLV TQHDAKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD
     AQFGGIDQRK IFTFAEKYLP TLGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED
     VKKKLKKAFC EPGNVENNGV LSFVKHVLFP LKSEFVILRD EKWGGNKTYT IYQELEKDFA
     AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPT AKGPAKSSEP
     EEIIPSRLDI RVGKILSVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV
     VVLCNLKPQK MRGVDSQGML LCASVEGVSR QVEPLDPPAG SAPGERVFVQ GYEKGQPDEE
     LKPKKKVFEK LQADFKISDD CVAQWKQTNF MTKLGFVSCK SLKGGNIS
//