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Protein

Tyrosine--tRNA ligase, cytoplasmic

Gene

Yars

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).By similarity

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39TyrosineBy similarity1
Binding sitei166TyrosineBy similarity1
Binding sitei170TyrosineBy similarity1
Binding sitei173TyrosineBy similarity1
Binding sitei188TyrosineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligase, cytoplasmic (EC:6.1.1.1)
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name:
TyrRS
Cleaved into the following chain:
Gene namesi
Name:Yars
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1307616. Yars.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002396911 – 528Tyrosine--tRNA ligase, cytoplasmicAdd BLAST528
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004232882 – 528Tyrosine--tRNA ligase, cytoplasmic, N-terminally processedAdd BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylglycine; in Tyrosine--tRNA ligase, cytoplasmic, N-terminally processedBy similarity1
Modified residuei197N6-acetyllysineBy similarity1
Modified residuei205PhosphoserineBy similarity1
Modified residuei206N6-acetyllysineBy similarity1
Modified residuei386PhosphoserineBy similarity1
Modified residuei474N6-acetyllysineBy similarity1
Modified residuei482N6-acetyllysineBy similarity1
Modified residuei490N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ4KM49.
PeptideAtlasiQ4KM49.
PRIDEiQ4KM49.

PTM databases

iPTMnetiQ4KM49.
PhosphoSitePlusiQ4KM49.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007213.
GenevisibleiQ4KM49. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi260303. 1 interactor.
STRINGi10116.ENSRNOP00000010674.

Structurei

3D structure databases

ProteinModelPortaliQ4KM49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini364 – 468tRNA-bindingPROSITE-ProRule annotationAdd BLAST105

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi44 – 52"HIGH" regionBy similarity9
Motifi222 – 226"KMSKS" regionBy similarity5

Sequence similaritiesi

Contains 1 tRNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2144. Eukaryota.
KOG2241. Eukaryota.
COG0073. LUCA.
COG0162. LUCA.
HOGENOMiHOG000228237.
HOVERGENiHBG080113.
InParanoidiQ4KM49.
KOiK01866.
OrthoDBiEOG091G03XO.
PhylomeDBiQ4KM49.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR002307. Tyr-tRNA-ligase.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4KM49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKVYW GTATTGKPHV
60 70 80 90 100
AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRTSYYENV
110 120 130 140 150
IKAMLESIGV PLEKLKFTKG TDYQLSKEYT LDVYRLSSLV TQHDAKKAGA
160 170 180 190 200
EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP
210 220 230 240 250
TLGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC
260 270 280 290 300
EPGNVENNGV LSFVKHVLFP LKSEFVILRD EKWGGNKTYT IYQELEKDFA
310 320 330 340 350
AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPT
360 370 380 390 400
AKGPAKSSEP EEIIPSRLDI RVGKILSVEK HPDADSLYVE KIDVGEAEPR
410 420 430 440 450
TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVDSQGML LCASVEGVSR
460 470 480 490 500
QVEPLDPPAG SAPGERVFVQ GYEKGQPDEE LKPKKKVFEK LQADFKISDD
510 520
CVAQWKQTNF MTKLGFVSCK SLKGGNIS
Length:528
Mass (Da):59,115
Last modified:January 23, 2007 - v3
Checksum:iAD79FC551BEC9CBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098795 mRNA. Translation: AAH98795.1.
RefSeqiNP_001020867.2. NM_001025696.1.
UniGeneiRn.41002.

Genome annotation databases

GeneIDi313047.
KEGGirno:313047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098795 mRNA. Translation: AAH98795.1.
RefSeqiNP_001020867.2. NM_001025696.1.
UniGeneiRn.41002.

3D structure databases

ProteinModelPortaliQ4KM49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi260303. 1 interactor.
STRINGi10116.ENSRNOP00000010674.

PTM databases

iPTMnetiQ4KM49.
PhosphoSitePlusiQ4KM49.

Proteomic databases

PaxDbiQ4KM49.
PeptideAtlasiQ4KM49.
PRIDEiQ4KM49.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi313047.
KEGGirno:313047.

Organism-specific databases

CTDi8565.
RGDi1307616. Yars.

Phylogenomic databases

eggNOGiKOG2144. Eukaryota.
KOG2241. Eukaryota.
COG0073. LUCA.
COG0162. LUCA.
HOGENOMiHOG000228237.
HOVERGENiHBG080113.
InParanoidiQ4KM49.
KOiK01866.
OrthoDBiEOG091G03XO.
PhylomeDBiQ4KM49.

Miscellaneous databases

PROiQ4KM49.

Gene expression databases

BgeeiENSRNOG00000007213.
GenevisibleiQ4KM49. RN.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR002307. Tyr-tRNA-ligase.
[Graphical view]
PfamiPF00579. tRNA-synt_1b. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYYC_RAT
AccessioniPrimary (citable) accession number: Q4KM49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.