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Q4KM49 (SYYC_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tyrosine--tRNA ligase, cytoplasmic
EC=6.1.1.1
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS
Gene names
Name:Yars
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.

Catalytic activity

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtyrosyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

tyrosine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 528527Tyrosine--tRNA ligase, cytoplasmic
PRO_0000239691

Regions

Domain364 – 468105tRNA-binding
Motif44 – 529"HIGH" region By similarity
Motif222 – 2265"KMSKS" region By similarity

Sites

Binding site391Tyrosine By similarity
Binding site1661Tyrosine By similarity
Binding site1701Tyrosine By similarity
Binding site1731Tyrosine By similarity
Binding site1881Tyrosine By similarity

Amino acid modifications

Modified residue21N-acetylglycine By similarity
Modified residue1971N6-acetyllysine By similarity
Modified residue2061N6-acetyllysine By similarity
Modified residue2721N6-acetyllysine By similarity
Modified residue4741N6-acetyllysine By similarity
Modified residue4821N6-acetyllysine By similarity
Modified residue4901N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4KM49 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: AD79FC551BEC9CBA

FASTA52859,115
        10         20         30         40         50         60 
MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKVYW GTATTGKPHV AYFVPMSKIA 

        70         80         90        100        110        120 
DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRTSYYENV IKAMLESIGV PLEKLKFTKG 

       130        140        150        160        170        180 
TDYQLSKEYT LDVYRLSSLV TQHDAKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD 

       190        200        210        220        230        240 
AQFGGIDQRK IFTFAEKYLP TLGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED 

       250        260        270        280        290        300 
VKKKLKKAFC EPGNVENNGV LSFVKHVLFP LKSEFVILRD EKWGGNKTYT IYQELEKDFA 

       310        320        330        340        350        360 
AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPT AKGPAKSSEP 

       370        380        390        400        410        420 
EEIIPSRLDI RVGKILSVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV 

       430        440        450        460        470        480 
VVLCNLKPQK MRGVDSQGML LCASVEGVSR QVEPLDPPAG SAPGERVFVQ GYEKGQPDEE 

       490        500        510        520 
LKPKKKVFEK LQADFKISDD CVAQWKQTNF MTKLGFVSCK SLKGGNIS

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC098795 mRNA. Translation: AAH98795.1.
IPIIPI00366785.
RefSeqNP_001020867.1. NM_001025696.1.
UniGeneRn.41002.

3D structure databases

ProteinModelPortalQ4KM49.
SMRQ4KM49. Positions 4-342, 360-528.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ4KM49.

PTM databases

PhosphoSiteQ4KM49.

Proteomic databases

PRIDEQ4KM49.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID313047.
KEGGrno:313047.
NMPDRfig|10116.3.peg.23990.
UCSCNM_001025696. rat.

Organism-specific databases

CTD8565.
RGD1307616. Yars.

Phylogenomic databases

eggNOGroNOG09132.
GeneTreeENSGT00550000074927.
HOVERGENHBG080113.
InParanoidQ4KM49.
OMAFCEPGNI.
OrthoDBEOG4RV2R7.
PhylomeDBQ4KM49.

Gene expression databases

ArrayExpressQ4KM49.
GenevestigatorQ4KM49.
GermOnlineENSRNOG00000007213. Rattus norvegicus.

Family and domain databases

InterProIPR002305. aa-tRNA-synth_Ic.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR002307. Tyr-tRNA-synth.
IPR023617. Tyr-tRNA-synth_arc-type.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01866.
PANTHERPTHR11946:SF8. Tyr-tRNA_synth. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01040. TRNASYNTHTYR.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00234. TyrS. 1 hit.
PROSITEPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio665526.

Entry information

Entry nameSYYC_RAT
AccessionPrimary (citable) accession number: Q4KM49
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 53 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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