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Q4KM35

- PSB10_RAT

UniProt

Q4KM35 - PSB10_RAT

Protein

Proteasome subunit beta type-10

Gene

Psmb10

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides By similarity.By similarity

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei39 – 402Cleavage; by autocatalysisBy similarity
    Active sitei40 – 401NucleophileBy similarity

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cell morphogenesis Source: Ensembl
    2. proteolysis involved in cellular protein catabolic process Source: InterPro
    3. T cell proliferation Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_194781. Separation of Sister Chromatids.
    REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_198391. Asymmetric localization of PCP proteins.
    REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_199197. ER-Phagosome pathway.
    REACT_199247. Activation of NF-kappaB in B cells.
    REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_206488. degradation of DVL.
    REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
    REACT_211117. Orc1 removal from chromatin.
    REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_220232. Regulation of ornithine decarboxylase (ODC).
    REACT_227706. degradation of AXIN.

    Protein family/group databases

    MEROPSiT01.014.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-10 (EC:3.4.25.1)
    Alternative name(s):
    Low molecular mass protein 10
    Macropain subunit MECl-1
    Multicatalytic endopeptidase complex subunit MECl-1
    Proteasome MECl-1
    Proteasome subunit beta-2i
    Gene namesi
    Name:Psmb10
    Synonyms:Lmp10, Mecl1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Organism-specific databases

    RGDi1307428. Psmb10.

    Subcellular locationi

    Cytoplasm. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: UniProtKB
    4. spermatoproteasome complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 3939Removed in mature formBy similarityPRO_0000391410Add
    BLAST
    Chaini40 – 273234Proteasome subunit beta type-10PRO_0000391411Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Post-translational modificationi

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ4KM35.
    PRIDEiQ4KM35.

    Expressioni

    Inductioni

    Up-regulated by interferon gamma. Up-regulated by theophylline (THP) and down-regulated by 1,3-dinitrobenzene (DNB), two reprotoxic agents thought to induce infertility.1 Publication

    Gene expression databases

    GenevestigatoriQ4KM35.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB7. Component of the spermatoproteasome, a form of the proteasome specifically found in testis By similarity.By similarity

    Protein-protein interaction databases

    BioGridi253721. 1 interaction.
    STRINGi10116.ENSRNOP00000026462.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4KM35.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    GeneTreeiENSGT00510000046533.
    HOGENOMiHOG000182856.
    HOVERGENiHBG093416.
    InParanoidiQ4KM35.
    KOiK02733.
    OMAiQYRFAPG.
    OrthoDBiEOG7CRTQJ.
    PhylomeDBiQ4KM35.
    TreeFamiTF106222.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR024689. Proteasome_bsu_C.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF12465. Pr_beta_C. 1 hit.
    PF00227. Proteasome. 1 hit.
    [Graphical view]
    PRINTSiPR00141. PROTEASOME.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4KM35-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKQAVEHRG GFSFENCQRN ASLEHVLPGL RVPLARKTGT TIAGLVFRDG    50
    VILGADTRAT NDSVVADKSC EKIHFIAPKI YCCGAGVAAD TEMTTRMAAS 100
    KMELHALSTG REPRVATVTR ILRQTLFRYQ GHVGASLIVG GVDLNGPQLY 150
    SVHPHGSYSR LPFTALGSGQ DAAVALLEDR FQPNMTLEAA QELLVEAITA 200
    GILGDLGSGG SVDACVITAG GAKLQRALSS PIEPVQRAGQ YRFAPGTTPV 250
    QTQEVRALTL ELLEETVQAM EVE 273
    Length:273
    Mass (Da):29,038
    Last modified:August 2, 2005 - v1
    Checksum:iBA3830BFDDFDC91B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098835 mRNA. Translation: AAH98835.1.
    RefSeqiNP_001020808.1. NM_001025637.1.
    UniGeneiRn.24968.

    Genome annotation databases

    EnsembliENSRNOT00000026462; ENSRNOP00000026462; ENSRNOG00000019494.
    GeneIDi291983.
    KEGGirno:291983.
    UCSCiRGD:1307428. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098835 mRNA. Translation: AAH98835.1 .
    RefSeqi NP_001020808.1. NM_001025637.1.
    UniGenei Rn.24968.

    3D structure databases

    ProteinModelPortali Q4KM35.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 253721. 1 interaction.
    STRINGi 10116.ENSRNOP00000026462.

    Protein family/group databases

    MEROPSi T01.014.

    Proteomic databases

    PaxDbi Q4KM35.
    PRIDEi Q4KM35.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000026462 ; ENSRNOP00000026462 ; ENSRNOG00000019494 .
    GeneIDi 291983.
    KEGGi rno:291983.
    UCSCi RGD:1307428. rat.

    Organism-specific databases

    CTDi 5699.
    RGDi 1307428. Psmb10.

    Phylogenomic databases

    eggNOGi COG0638.
    GeneTreei ENSGT00510000046533.
    HOGENOMi HOG000182856.
    HOVERGENi HBG093416.
    InParanoidi Q4KM35.
    KOi K02733.
    OMAi QYRFAPG.
    OrthoDBi EOG7CRTQJ.
    PhylomeDBi Q4KM35.
    TreeFami TF106222.

    Enzyme and pathway databases

    Reactomei REACT_194781. Separation of Sister Chromatids.
    REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_198391. Asymmetric localization of PCP proteins.
    REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_199197. ER-Phagosome pathway.
    REACT_199247. Activation of NF-kappaB in B cells.
    REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
    REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_206488. degradation of DVL.
    REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
    REACT_211117. Orc1 removal from chromatin.
    REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
    REACT_220232. Regulation of ornithine decarboxylase (ODC).
    REACT_227706. degradation of AXIN.

    Miscellaneous databases

    NextBioi 633556.
    PROi Q4KM35.

    Gene expression databases

    Genevestigatori Q4KM35.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR000243. Pept_T1A_subB.
    IPR024689. Proteasome_bsu_C.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF12465. Pr_beta_C. 1 hit.
    PF00227. Proteasome. 1 hit.
    [Graphical view ]
    PRINTSi PR00141. PROTEASOME.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Spleen.
    2. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
      Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
      Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY THP AND DNP.

    Entry informationi

    Entry nameiPSB10_RAT
    AccessioniPrimary (citable) accession number: Q4KM35
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 9, 2010
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3