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Q4KM35

- PSB10_RAT

UniProt

Q4KM35 - PSB10_RAT

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Protein

Proteasome subunit beta type-10

Gene

Psmb10

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei39 – 402Cleavage; by autocatalysisBy similarity
Active sitei40 – 401NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. cell morphogenesis Source: Ensembl
  2. proteolysis involved in cellular protein catabolic process Source: InterPro
  3. T cell proliferation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiREACT_194781. Separation of Sister Chromatids.
REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_198391. Asymmetric localization of PCP proteins.
REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_199197. ER-Phagosome pathway.
REACT_199247. Activation of NF-kappaB in B cells.
REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_206488. degradation of DVL.
REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
REACT_211117. Orc1 removal from chromatin.
REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
REACT_220232. Regulation of ornithine decarboxylase (ODC).
REACT_227706. degradation of AXIN.
REACT_235738. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_236654. Hedgehog ligand biogenesis.
REACT_240793. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_244609. SCF-beta-TrCP mediated degradation of Emi1.
REACT_245329. APC/C:Cdc20 mediated degradation of Securin.
REACT_246662. SCF(Skp2)-mediated degradation of p27/p21.
REACT_247625. Ubiquitin-dependent degradation of Cyclin D1.
REACT_252335. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_255131. Hh ligand biogenesis disease.
REACT_257365. Degradation of beta-catenin by the destruction complex.
REACT_259430. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_269272. Degradation of GLI1 by the proteasome.
REACT_269753. GLI3 is processed to GLI3R by the proteasome.
REACT_270919. Degradation of GLI2 by the proteasome.

Protein family/group databases

MEROPSiT01.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-10 (EC:3.4.25.1)
Alternative name(s):
Low molecular mass protein 10
Macropain subunit MECl-1
Multicatalytic endopeptidase complex subunit MECl-1
Proteasome MECl-1
Proteasome subunit beta-2i
Gene namesi
Name:Psmb10
Synonyms:Lmp10, Mecl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 19

Organism-specific databases

RGDi1307428. Psmb10.

Subcellular locationi

Cytoplasm. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. proteasome core complex Source: UniProtKB
  4. spermatoproteasome complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 3939Removed in mature formBy similarityPRO_0000391410Add
BLAST
Chaini40 – 273234Proteasome subunit beta type-10PRO_0000391411Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ4KM35.
PRIDEiQ4KM35.

Expressioni

Inductioni

Up-regulated by interferon gamma. Up-regulated by theophylline (THP) and down-regulated by 1,3-dinitrobenzene (DNB), two reprotoxic agents thought to induce infertility.1 Publication

Gene expression databases

GenevestigatoriQ4KM35.

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB7. Component of the spermatoproteasome, a form of the proteasome specifically found in testis (By similarity).By similarity

Protein-protein interaction databases

BioGridi253721. 1 interaction.
STRINGi10116.ENSRNOP00000026462.

Structurei

3D structure databases

ProteinModelPortaliQ4KM35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
GeneTreeiENSGT00510000046533.
HOGENOMiHOG000182856.
HOVERGENiHBG093416.
InParanoidiQ4KM35.
KOiK02733.
OMAiQYRFAPG.
OrthoDBiEOG7CRTQJ.
PhylomeDBiQ4KM35.
TreeFamiTF106222.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR024689. Proteasome_bsu_C.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF12465. Pr_beta_C. 1 hit.
PF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4KM35-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKQAVEHRG GFSFENCQRN ASLEHVLPGL RVPLARKTGT TIAGLVFRDG
60 70 80 90 100
VILGADTRAT NDSVVADKSC EKIHFIAPKI YCCGAGVAAD TEMTTRMAAS
110 120 130 140 150
KMELHALSTG REPRVATVTR ILRQTLFRYQ GHVGASLIVG GVDLNGPQLY
160 170 180 190 200
SVHPHGSYSR LPFTALGSGQ DAAVALLEDR FQPNMTLEAA QELLVEAITA
210 220 230 240 250
GILGDLGSGG SVDACVITAG GAKLQRALSS PIEPVQRAGQ YRFAPGTTPV
260 270
QTQEVRALTL ELLEETVQAM EVE
Length:273
Mass (Da):29,038
Last modified:August 2, 2005 - v1
Checksum:iBA3830BFDDFDC91B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098835 mRNA. Translation: AAH98835.1.
RefSeqiNP_001020808.1. NM_001025637.1.
UniGeneiRn.24968.

Genome annotation databases

EnsembliENSRNOT00000026462; ENSRNOP00000026462; ENSRNOG00000019494.
GeneIDi291983.
KEGGirno:291983.
UCSCiRGD:1307428. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098835 mRNA. Translation: AAH98835.1 .
RefSeqi NP_001020808.1. NM_001025637.1.
UniGenei Rn.24968.

3D structure databases

ProteinModelPortali Q4KM35.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 253721. 1 interaction.
STRINGi 10116.ENSRNOP00000026462.

Protein family/group databases

MEROPSi T01.014.

Proteomic databases

PaxDbi Q4KM35.
PRIDEi Q4KM35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000026462 ; ENSRNOP00000026462 ; ENSRNOG00000019494 .
GeneIDi 291983.
KEGGi rno:291983.
UCSCi RGD:1307428. rat.

Organism-specific databases

CTDi 5699.
RGDi 1307428. Psmb10.

Phylogenomic databases

eggNOGi COG0638.
GeneTreei ENSGT00510000046533.
HOGENOMi HOG000182856.
HOVERGENi HBG093416.
InParanoidi Q4KM35.
KOi K02733.
OMAi QYRFAPG.
OrthoDBi EOG7CRTQJ.
PhylomeDBi Q4KM35.
TreeFami TF106222.

Enzyme and pathway databases

Reactomei REACT_194781. Separation of Sister Chromatids.
REACT_196424. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_198391. Asymmetric localization of PCP proteins.
REACT_199194. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_199197. ER-Phagosome pathway.
REACT_199247. Activation of NF-kappaB in B cells.
REACT_199254. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_204983. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_206488. degradation of DVL.
REACT_206997. CDT1 association with the CDC6:ORC:origin complex.
REACT_211117. Orc1 removal from chromatin.
REACT_212486. CDK-mediated phosphorylation and removal of Cdc6.
REACT_220232. Regulation of ornithine decarboxylase (ODC).
REACT_227706. degradation of AXIN.
REACT_235738. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_236654. Hedgehog ligand biogenesis.
REACT_240793. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_244609. SCF-beta-TrCP mediated degradation of Emi1.
REACT_245329. APC/C:Cdc20 mediated degradation of Securin.
REACT_246662. SCF(Skp2)-mediated degradation of p27/p21.
REACT_247625. Ubiquitin-dependent degradation of Cyclin D1.
REACT_252335. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_255131. Hh ligand biogenesis disease.
REACT_257365. Degradation of beta-catenin by the destruction complex.
REACT_259430. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_269272. Degradation of GLI1 by the proteasome.
REACT_269753. GLI3 is processed to GLI3R by the proteasome.
REACT_270919. Degradation of GLI2 by the proteasome.

Miscellaneous databases

NextBioi 633556.
PROi Q4KM35.

Gene expression databases

Genevestigatori Q4KM35.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR024689. Proteasome_bsu_C.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF12465. Pr_beta_C. 1 hit.
PF00227. Proteasome. 1 hit.
[Graphical view ]
PRINTSi PR00141. PROTEASOME.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  2. "Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
    Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
    Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY THP AND DNP.

Entry informationi

Entry nameiPSB10_RAT
AccessioniPrimary (citable) accession number: Q4KM35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: August 2, 2005
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3