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Q4KM35 (PSB10_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-10

EC=3.4.25.1
Alternative name(s):
Low molecular mass protein 10
Macropain subunit MECl-1
Multicatalytic endopeptidase complex subunit MECl-1
Proteasome MECl-1
Proteasome subunit beta-2i
Gene names
Name:Psmb10
Synonyms:Lmp10, Mecl1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides By similarity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB7. Component of the spermatoproteasome, a form of the proteasome specifically found in testis By similarity.

Subcellular location

Cytoplasm. Nucleus By similarity.

Induction

Up-regulated by interferon gamma. Up-regulated by theophylline (THP) and down-regulated by 1,3-dinitrobenzene (DNB), two reprotoxic agents thought to induce infertility. Ref.2

Post-translational modification

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity By similarity.

Sequence similarities

Belongs to the peptidase T1B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 3939Removed in mature form By similarity
PRO_0000391410
Chain40 – 273234Proteasome subunit beta type-10
PRO_0000391411

Sites

Active site401Nucleophile By similarity
Site39 – 402Cleavage; by autocatalysis By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4KM35 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: BA3830BFDDFDC91B

FASTA27329,038
        10         20         30         40         50         60 
MLKQAVEHRG GFSFENCQRN ASLEHVLPGL RVPLARKTGT TIAGLVFRDG VILGADTRAT 

        70         80         90        100        110        120 
NDSVVADKSC EKIHFIAPKI YCCGAGVAAD TEMTTRMAAS KMELHALSTG REPRVATVTR 

       130        140        150        160        170        180 
ILRQTLFRYQ GHVGASLIVG GVDLNGPQLY SVHPHGSYSR LPFTALGSGQ DAAVALLEDR 

       190        200        210        220        230        240 
FQPNMTLEAA QELLVEAITA GILGDLGSGG SVDACVITAG GAKLQRALSS PIEPVQRAGQ 

       250        260        270 
YRFAPGTTPV QTQEVRALTL ELLEETVQAM EVE 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
[2]"Differential expression of genes encoding constitutive and inducible 20S proteasomal core subunits in the testis and epididymis of theophylline- or 1,3-dinitrobenzene-exposed rats."
Tengowski M.W., Feng D., Sutovsky M., Sutovsky P.
Biol. Reprod. 76:149-163(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY THP AND DNP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC098835 mRNA. Translation: AAH98835.1.
RefSeqNP_001020808.1. NM_001025637.1.
UniGeneRn.24968.

3D structure databases

ProteinModelPortalQ4KM35.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid253721. 1 interaction.
STRING10116.ENSRNOP00000026462.

Protein family/group databases

MEROPST01.014.

Proteomic databases

PaxDbQ4KM35.
PRIDEQ4KM35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026462; ENSRNOP00000026462; ENSRNOG00000019494.
GeneID291983.
KEGGrno:291983.
UCSCRGD:1307428. rat.

Organism-specific databases

CTD5699.
RGD1307428. Psmb10.

Phylogenomic databases

eggNOGCOG0638.
GeneTreeENSGT00510000046533.
HOGENOMHOG000182856.
HOVERGENHBG093416.
InParanoidQ4KM35.
KOK02733.
OMAQYRFAPG.
OrthoDBEOG7CRTQJ.
PhylomeDBQ4KM35.
TreeFamTF106222.

Gene expression databases

GenevestigatorQ4KM35.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR024689. Proteasome_bsu_C.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF12465. Pr_beta_C. 1 hit.
PF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio633556.
PROQ4KM35.

Entry information

Entry namePSB10_RAT
AccessionPrimary (citable) accession number: Q4KM35
Entry history
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: August 2, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries