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Q4KM32

- MUS81_RAT

UniProt

Q4KM32 - MUS81_RAT

Protein

Crossover junction endonuclease MUS81

Gene

Mus81

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (02 Aug 2005)
      Previous versions | rss
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    Functioni

    Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks By similarity.By similarity

    Cofactori

    Magnesium.By similarity

    GO - Molecular functioni

    1. 3'-flap endonuclease activity Source: Ensembl
    2. DNA binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endonuclease MUS81 (EC:3.1.22.-)
    Gene namesi
    Name:Mus81
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi1311957. Mus81.

    Subcellular locationi

    Nucleusnucleolus
    Note: Recruited to foci of DNA damage in S-phase cells.By similarity

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551Crossover junction endonuclease MUS81PRO_0000223636Add
    BLAST

    Proteomic databases

    PRIDEiQ4KM32.

    Expressioni

    Gene expression databases

    GenevestigatoriQ4KM32.

    Interactioni

    Subunit structurei

    May self-associate. Interacts with EME1, EME2 and CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028015.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4KM32.
    SMRiQ4KM32. Positions 259-551.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini270 – 372103ERCC4Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 244120Interaction with BLMBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the XPF family.Curated
    Contains 1 ERCC4 domain.Curated

    Phylogenomic databases

    eggNOGiCOG1948.
    GeneTreeiENSGT00390000005498.
    HOGENOMiHOG000113699.
    HOVERGENiHBG052538.
    InParanoidiQ4KM32.
    KOiK08991.
    OMAiKCGRLQR.
    OrthoDBiEOG7TXKGV.
    PhylomeDBiQ4KM32.
    TreeFamiTF315113.

    Family and domain databases

    Gene3Di1.10.8.310. 1 hit.
    3.40.50.10130. 1 hit.
    InterProiIPR010996. DNA_pol_b-like_N.
    IPR020819. DNA_repair_nuc_XPF/helicase.
    IPR006166. ERCC4_domain.
    IPR027420. PolB_N.
    IPR011335. Restrct_endonuc-II-like.
    [Graphical view]
    PfamiPF02732. ERCC4. 1 hit.
    [Graphical view]
    SMARTiSM00891. ERCC4. 1 hit.
    [Graphical view]
    SUPFAMiSSF47802. SSF47802. 1 hit.
    SSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4KM32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEPVRLGRK RPLPVCPNPL FVRWLTEWRD EAASRGRHTR FVFQKALRSL    50
    QRYPLPLRNG KEAKILQHFG DRLCRMLDER LKEHLASGGD HAPSSPSGKK 100
    RASKGPPAQV QGSSMPVPTQ PQAGSTNAGY WPAQNSGARE ILLQLYREHL 150
    NSDGHSFLTK EELLQKCAQK TPRVVPESSR PWPALRGLLH RNLVLRTHRP 200
    ARYALTPEGL ELAQKLAEAE GLSTLNTAFQ PEEHHEESPV PEAILSEPGT 250
    TEVGVQQRPL ELRPSEYRVL LCVDIGETRG AGHRPEMLRE LQRLRVPHTV 300
    RKLHVGDFVW VAQETRPRDP ERPGELVLDH IVERKRLDDL CSSIIDGRFR 350
    EQKFRLKRCG LGHRIYLVEE HGSVQNLSLP ESTLLQAVTN TQVIDGFFVK 400
    RTMDIKESAG YLALLTKGLE RLYQGHTLHS RPWGTPGDAE SEAKPSTNPL 450
    CSLLTFSDFN AEAVKNKAQS VREVFARQLM QVRGLSGEKA AALVDRYSTP 500
    ASLLAAYDAC ATTKEQEMLL STVKCGRLQR NLGPALSRTL YQLYCSHSPL 550
    T 551
    Length:551
    Mass (Da):61,903
    Last modified:August 2, 2005 - v1
    Checksum:i3F42CC6A4E6559C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098853 mRNA. Translation: AAH98853.1.
    RefSeqiNP_001020816.1. NM_001025645.1.
    UniGeneiRn.137652.

    Genome annotation databases

    EnsembliENSRNOT00000028015; ENSRNOP00000028015; ENSRNOG00000020617.
    GeneIDi293678.
    KEGGirno:293678.
    UCSCiRGD:1311957. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC098853 mRNA. Translation: AAH98853.1 .
    RefSeqi NP_001020816.1. NM_001025645.1.
    UniGenei Rn.137652.

    3D structure databases

    ProteinModelPortali Q4KM32.
    SMRi Q4KM32. Positions 259-551.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000028015.

    Proteomic databases

    PRIDEi Q4KM32.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028015 ; ENSRNOP00000028015 ; ENSRNOG00000020617 .
    GeneIDi 293678.
    KEGGi rno:293678.
    UCSCi RGD:1311957. rat.

    Organism-specific databases

    CTDi 80198.
    RGDi 1311957. Mus81.

    Phylogenomic databases

    eggNOGi COG1948.
    GeneTreei ENSGT00390000005498.
    HOGENOMi HOG000113699.
    HOVERGENi HBG052538.
    InParanoidi Q4KM32.
    KOi K08991.
    OMAi KCGRLQR.
    OrthoDBi EOG7TXKGV.
    PhylomeDBi Q4KM32.
    TreeFami TF315113.

    Miscellaneous databases

    NextBioi 636725.
    PROi Q4KM32.

    Gene expression databases

    Genevestigatori Q4KM32.

    Family and domain databases

    Gene3Di 1.10.8.310. 1 hit.
    3.40.50.10130. 1 hit.
    InterProi IPR010996. DNA_pol_b-like_N.
    IPR020819. DNA_repair_nuc_XPF/helicase.
    IPR006166. ERCC4_domain.
    IPR027420. PolB_N.
    IPR011335. Restrct_endonuc-II-like.
    [Graphical view ]
    Pfami PF02732. ERCC4. 1 hit.
    [Graphical view ]
    SMARTi SM00891. ERCC4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47802. SSF47802. 1 hit.
    SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.

    Entry informationi

    Entry nameiMUS81_RAT
    AccessioniPrimary (citable) accession number: Q4KM32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2006
    Last sequence update: August 2, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3