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Q4KM32 (MUS81_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endonuclease MUS81

EC=3.1.22.-
Gene names
Name:Mus81
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks By similarity.

Cofactor

Magnesium By similarity.

Subunit structure

May self-associate. Interacts with EME1, EME2 and CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo By similarity.

Subcellular location

Nucleusnucleolus. Note: Recruited to foci of DNA damage in S-phase cells By similarity.

Sequence similarities

Belongs to the XPF family.

Contains 1 ERCC4 domain.

Ontologies

Keywords
   Biological processDNA damage
DNA recombination
DNA repair
   Cellular componentNucleus
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA recombination

Inferred from electronic annotation. Source: UniProtKB-KW

DNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function3'-flap endonuclease activity

Inferred from electronic annotation. Source: Ensembl

DNA binding

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Crossover junction endonuclease MUS81
PRO_0000223636

Regions

Domain270 – 372103ERCC4
Region125 – 244120Interaction with BLM By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4KM32 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 3F42CC6A4E6559C1

FASTA55161,903
        10         20         30         40         50         60 
MAEPVRLGRK RPLPVCPNPL FVRWLTEWRD EAASRGRHTR FVFQKALRSL QRYPLPLRNG 

        70         80         90        100        110        120 
KEAKILQHFG DRLCRMLDER LKEHLASGGD HAPSSPSGKK RASKGPPAQV QGSSMPVPTQ 

       130        140        150        160        170        180 
PQAGSTNAGY WPAQNSGARE ILLQLYREHL NSDGHSFLTK EELLQKCAQK TPRVVPESSR 

       190        200        210        220        230        240 
PWPALRGLLH RNLVLRTHRP ARYALTPEGL ELAQKLAEAE GLSTLNTAFQ PEEHHEESPV 

       250        260        270        280        290        300 
PEAILSEPGT TEVGVQQRPL ELRPSEYRVL LCVDIGETRG AGHRPEMLRE LQRLRVPHTV 

       310        320        330        340        350        360 
RKLHVGDFVW VAQETRPRDP ERPGELVLDH IVERKRLDDL CSSIIDGRFR EQKFRLKRCG 

       370        380        390        400        410        420 
LGHRIYLVEE HGSVQNLSLP ESTLLQAVTN TQVIDGFFVK RTMDIKESAG YLALLTKGLE 

       430        440        450        460        470        480 
RLYQGHTLHS RPWGTPGDAE SEAKPSTNPL CSLLTFSDFN AEAVKNKAQS VREVFARQLM 

       490        500        510        520        530        540 
QVRGLSGEKA AALVDRYSTP ASLLAAYDAC ATTKEQEMLL STVKCGRLQR NLGPALSRTL 

       550 
YQLYCSHSPL T 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC098853 mRNA. Translation: AAH98853.1.
RefSeqNP_001020816.1. NM_001025645.1.
UniGeneRn.137652.

3D structure databases

ProteinModelPortalQ4KM32.
SMRQ4KM32. Positions 259-551.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000028015.

Proteomic databases

PRIDEQ4KM32.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028015; ENSRNOP00000028015; ENSRNOG00000020617.
GeneID293678.
KEGGrno:293678.
UCSCRGD:1311957. rat.

Organism-specific databases

CTD80198.
RGD1311957. Mus81.

Phylogenomic databases

eggNOGCOG1948.
GeneTreeENSGT00390000005498.
HOGENOMHOG000113699.
HOVERGENHBG052538.
InParanoidQ4KM32.
KOK08991.
OMAKCGRLQR.
OrthoDBEOG7TXKGV.
PhylomeDBQ4KM32.
TreeFamTF315113.

Gene expression databases

GenevestigatorQ4KM32.

Family and domain databases

Gene3D1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

NextBio636725.
PROQ4KM32.

Entry information

Entry nameMUS81_RAT
AccessionPrimary (citable) accession number: Q4KM32
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families