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Protein

Crossover junction endonuclease MUS81

Gene

Mus81

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks (By similarity).By similarity

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-RNO-6783310 Fanconi Anemia Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease MUS81 (EC:3.1.22.-)
Gene namesi
Name:Mus81
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1311957 Mus81

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002236361 – 551Crossover junction endonuclease MUS81Add BLAST551

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei95PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4KM32
PRIDEiQ4KM32

Expressioni

Gene expression databases

BgeeiENSRNOG00000020617
GenevisibleiQ4KM32 RN

Interactioni

Subunit structurei

May self-associate. Interacts with EME1, EME2 and CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028015

Structurei

3D structure databases

ProteinModelPortaliQ4KM32
SMRiQ4KM32
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini270 – 372ERCC4Add BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 244Interaction with BLMBy similarityAdd BLAST120

Sequence similaritiesi

Belongs to the XPF family.Curated

Phylogenomic databases

eggNOGiKOG2379 Eukaryota
COG1948 LUCA
GeneTreeiENSGT00390000005498
HOGENOMiHOG000113699
HOVERGENiHBG052538
InParanoidiQ4KM32
KOiK08991
OMAiKCGRLQR
OrthoDBiEOG091G09T6
PhylomeDBiQ4KM32
TreeFamiTF315113

Family and domain databases

Gene3Di1.10.10.10, 1 hit
1.10.150.110, 1 hit
InterProiView protein in InterPro
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR006166 ERCC4_domain
IPR011335 Restrct_endonuc-II-like
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF02732 ERCC4, 1 hit
SMARTiView protein in SMART
SM00891 ERCC4, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52980 SSF52980, 1 hit

Sequencei

Sequence statusi: Complete.

Q4KM32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPVRLGRK RPLPVCPNPL FVRWLTEWRD EAASRGRHTR FVFQKALRSL
60 70 80 90 100
QRYPLPLRNG KEAKILQHFG DRLCRMLDER LKEHLASGGD HAPSSPSGKK
110 120 130 140 150
RASKGPPAQV QGSSMPVPTQ PQAGSTNAGY WPAQNSGARE ILLQLYREHL
160 170 180 190 200
NSDGHSFLTK EELLQKCAQK TPRVVPESSR PWPALRGLLH RNLVLRTHRP
210 220 230 240 250
ARYALTPEGL ELAQKLAEAE GLSTLNTAFQ PEEHHEESPV PEAILSEPGT
260 270 280 290 300
TEVGVQQRPL ELRPSEYRVL LCVDIGETRG AGHRPEMLRE LQRLRVPHTV
310 320 330 340 350
RKLHVGDFVW VAQETRPRDP ERPGELVLDH IVERKRLDDL CSSIIDGRFR
360 370 380 390 400
EQKFRLKRCG LGHRIYLVEE HGSVQNLSLP ESTLLQAVTN TQVIDGFFVK
410 420 430 440 450
RTMDIKESAG YLALLTKGLE RLYQGHTLHS RPWGTPGDAE SEAKPSTNPL
460 470 480 490 500
CSLLTFSDFN AEAVKNKAQS VREVFARQLM QVRGLSGEKA AALVDRYSTP
510 520 530 540 550
ASLLAAYDAC ATTKEQEMLL STVKCGRLQR NLGPALSRTL YQLYCSHSPL

T
Length:551
Mass (Da):61,903
Last modified:August 2, 2005 - v1
Checksum:i3F42CC6A4E6559C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098853 mRNA Translation: AAH98853.1
RefSeqiNP_001020816.1, NM_001025645.1
UniGeneiRn.137652

Genome annotation databases

EnsembliENSRNOT00000028015; ENSRNOP00000028015; ENSRNOG00000020617
GeneIDi293678
KEGGirno:293678
UCSCiRGD:1311957 rat

Similar proteinsi

Entry informationi

Entry nameiMUS81_RAT
AccessioniPrimary (citable) accession number: Q4KM32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: August 2, 2005
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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