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Q4KM32

- MUS81_RAT

UniProt

Q4KM32 - MUS81_RAT

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Protein
Crossover junction endonuclease MUS81
Gene
Mus81
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks By similarity.

Cofactori

Magnesium By similarity.

GO - Molecular functioni

  1. 3'-flap endonuclease activity Source: Ensembl
  2. DNA binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease MUS81 (EC:3.1.22.-)
Gene namesi
Name:Mus81
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi1311957. Mus81.

Subcellular locationi

Nucleusnucleolus
Note: Recruited to foci of DNA damage in S-phase cells By similarity.

GO - Cellular componenti

  1. nucleolus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551Crossover junction endonuclease MUS81
PRO_0000223636Add
BLAST

Proteomic databases

PRIDEiQ4KM32.

Expressioni

Gene expression databases

GenevestigatoriQ4KM32.

Interactioni

Subunit structurei

May self-associate. Interacts with EME1, EME2 and CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028015.

Structurei

3D structure databases

ProteinModelPortaliQ4KM32.
SMRiQ4KM32. Positions 259-551.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini270 – 372103ERCC4
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 244120Interaction with BLM By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the XPF family.
Contains 1 ERCC4 domain.

Phylogenomic databases

eggNOGiCOG1948.
GeneTreeiENSGT00390000005498.
HOGENOMiHOG000113699.
HOVERGENiHBG052538.
InParanoidiQ4KM32.
KOiK08991.
OMAiKCGRLQR.
OrthoDBiEOG7TXKGV.
PhylomeDBiQ4KM32.
TreeFamiTF315113.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4KM32-1 [UniParc]FASTAAdd to Basket

« Hide

MAEPVRLGRK RPLPVCPNPL FVRWLTEWRD EAASRGRHTR FVFQKALRSL    50
QRYPLPLRNG KEAKILQHFG DRLCRMLDER LKEHLASGGD HAPSSPSGKK 100
RASKGPPAQV QGSSMPVPTQ PQAGSTNAGY WPAQNSGARE ILLQLYREHL 150
NSDGHSFLTK EELLQKCAQK TPRVVPESSR PWPALRGLLH RNLVLRTHRP 200
ARYALTPEGL ELAQKLAEAE GLSTLNTAFQ PEEHHEESPV PEAILSEPGT 250
TEVGVQQRPL ELRPSEYRVL LCVDIGETRG AGHRPEMLRE LQRLRVPHTV 300
RKLHVGDFVW VAQETRPRDP ERPGELVLDH IVERKRLDDL CSSIIDGRFR 350
EQKFRLKRCG LGHRIYLVEE HGSVQNLSLP ESTLLQAVTN TQVIDGFFVK 400
RTMDIKESAG YLALLTKGLE RLYQGHTLHS RPWGTPGDAE SEAKPSTNPL 450
CSLLTFSDFN AEAVKNKAQS VREVFARQLM QVRGLSGEKA AALVDRYSTP 500
ASLLAAYDAC ATTKEQEMLL STVKCGRLQR NLGPALSRTL YQLYCSHSPL 550
T 551
Length:551
Mass (Da):61,903
Last modified:August 2, 2005 - v1
Checksum:i3F42CC6A4E6559C1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC098853 mRNA. Translation: AAH98853.1.
RefSeqiNP_001020816.1. NM_001025645.1.
UniGeneiRn.137652.

Genome annotation databases

EnsembliENSRNOT00000028015; ENSRNOP00000028015; ENSRNOG00000020617.
GeneIDi293678.
KEGGirno:293678.
UCSCiRGD:1311957. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC098853 mRNA. Translation: AAH98853.1 .
RefSeqi NP_001020816.1. NM_001025645.1.
UniGenei Rn.137652.

3D structure databases

ProteinModelPortali Q4KM32.
SMRi Q4KM32. Positions 259-551.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000028015.

Proteomic databases

PRIDEi Q4KM32.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000028015 ; ENSRNOP00000028015 ; ENSRNOG00000020617 .
GeneIDi 293678.
KEGGi rno:293678.
UCSCi RGD:1311957. rat.

Organism-specific databases

CTDi 80198.
RGDi 1311957. Mus81.

Phylogenomic databases

eggNOGi COG1948.
GeneTreei ENSGT00390000005498.
HOGENOMi HOG000113699.
HOVERGENi HBG052538.
InParanoidi Q4KM32.
KOi K08991.
OMAi KCGRLQR.
OrthoDBi EOG7TXKGV.
PhylomeDBi Q4KM32.
TreeFami TF315113.

Miscellaneous databases

NextBioi 636725.
PROi Q4KM32.

Gene expression databases

Genevestigatori Q4KM32.

Family and domain databases

Gene3Di 1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProi IPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view ]
Pfami PF02732. ERCC4. 1 hit.
[Graphical view ]
SMARTi SM00891. ERCC4. 1 hit.
[Graphical view ]
SUPFAMi SSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.

Entry informationi

Entry nameiMUS81_RAT
AccessioniPrimary (citable) accession number: Q4KM32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: August 2, 2005
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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