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Protein

Crossover junction endonuclease MUS81

Gene

Mus81

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks (By similarity).By similarity

Cofactori

Mg2+By similarity

GO - Molecular functioni

  1. 3'-flap endonuclease activity Source: Ensembl
  2. DNA binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
  3. response to intra-S DNA damage checkpoint signaling Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease MUS81 (EC:3.1.22.-)
Gene namesi
Name:Mus81
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1311957. Mus81.

Subcellular locationi

Nucleusnucleolus
Note: Recruited to foci of DNA damage in S-phase cells.By similarity

GO - Cellular componenti

  1. intercellular bridge Source: Ensembl
  2. nucleolus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551Crossover junction endonuclease MUS81PRO_0000223636Add
BLAST

Proteomic databases

PRIDEiQ4KM32.

Expressioni

Gene expression databases

GenevestigatoriQ4KM32.

Interactioni

Subunit structurei

May self-associate. Interacts with EME1, EME2 and CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028015.

Structurei

3D structure databases

ProteinModelPortaliQ4KM32.
SMRiQ4KM32. Positions 259-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini270 – 372103ERCC4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 244120Interaction with BLMBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the XPF family.Curated
Contains 1 ERCC4 domain.Curated

Phylogenomic databases

eggNOGiCOG1948.
GeneTreeiENSGT00390000005498.
HOGENOMiHOG000113699.
HOVERGENiHBG052538.
InParanoidiQ4KM32.
KOiK08991.
OMAiTFSDFNA.
OrthoDBiEOG7TXKGV.
PhylomeDBiQ4KM32.
TreeFamiTF315113.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4KM32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPVRLGRK RPLPVCPNPL FVRWLTEWRD EAASRGRHTR FVFQKALRSL
60 70 80 90 100
QRYPLPLRNG KEAKILQHFG DRLCRMLDER LKEHLASGGD HAPSSPSGKK
110 120 130 140 150
RASKGPPAQV QGSSMPVPTQ PQAGSTNAGY WPAQNSGARE ILLQLYREHL
160 170 180 190 200
NSDGHSFLTK EELLQKCAQK TPRVVPESSR PWPALRGLLH RNLVLRTHRP
210 220 230 240 250
ARYALTPEGL ELAQKLAEAE GLSTLNTAFQ PEEHHEESPV PEAILSEPGT
260 270 280 290 300
TEVGVQQRPL ELRPSEYRVL LCVDIGETRG AGHRPEMLRE LQRLRVPHTV
310 320 330 340 350
RKLHVGDFVW VAQETRPRDP ERPGELVLDH IVERKRLDDL CSSIIDGRFR
360 370 380 390 400
EQKFRLKRCG LGHRIYLVEE HGSVQNLSLP ESTLLQAVTN TQVIDGFFVK
410 420 430 440 450
RTMDIKESAG YLALLTKGLE RLYQGHTLHS RPWGTPGDAE SEAKPSTNPL
460 470 480 490 500
CSLLTFSDFN AEAVKNKAQS VREVFARQLM QVRGLSGEKA AALVDRYSTP
510 520 530 540 550
ASLLAAYDAC ATTKEQEMLL STVKCGRLQR NLGPALSRTL YQLYCSHSPL

T
Length:551
Mass (Da):61,903
Last modified:August 1, 2005 - v1
Checksum:i3F42CC6A4E6559C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098853 mRNA. Translation: AAH98853.1.
RefSeqiNP_001020816.1. NM_001025645.1.
UniGeneiRn.137652.

Genome annotation databases

EnsembliENSRNOT00000028015; ENSRNOP00000028015; ENSRNOG00000020617.
GeneIDi293678.
KEGGirno:293678.
UCSCiRGD:1311957. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098853 mRNA. Translation: AAH98853.1.
RefSeqiNP_001020816.1. NM_001025645.1.
UniGeneiRn.137652.

3D structure databases

ProteinModelPortaliQ4KM32.
SMRiQ4KM32. Positions 259-551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028015.

Proteomic databases

PRIDEiQ4KM32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028015; ENSRNOP00000028015; ENSRNOG00000020617.
GeneIDi293678.
KEGGirno:293678.
UCSCiRGD:1311957. rat.

Organism-specific databases

CTDi80198.
RGDi1311957. Mus81.

Phylogenomic databases

eggNOGiCOG1948.
GeneTreeiENSGT00390000005498.
HOGENOMiHOG000113699.
HOVERGENiHBG052538.
InParanoidiQ4KM32.
KOiK08991.
OMAiTFSDFNA.
OrthoDBiEOG7TXKGV.
PhylomeDBiQ4KM32.
TreeFamiTF315113.

Miscellaneous databases

NextBioi636725.
PROiQ4KM32.

Gene expression databases

GenevestigatoriQ4KM32.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.

Entry informationi

Entry nameiMUS81_RAT
AccessioniPrimary (citable) accession number: Q4KM32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2006
Last sequence update: August 1, 2005
Last modified: January 6, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.