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Protein

Triokinase/FMN cyclase

Gene

Tkfc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate (PubMed:16289032). Represses IFIH1-mediated cellular antiviral response (By similarity).By similarity1 Publication

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.
FAD = AMP + riboflavin cyclic-4',5'-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • Mn2+1 Publication, Co2+1 PublicationNote: Manganese or cobalt are requested for FAD-AMP lyase activity.1 Publication

Enzyme regulationi

Each activity is inhibited by the substrate(s) of the other.

Kineticsi

  1. KM=8.8 µM for FAD with manganese1 Publication
  2. KM=12.5 µM for ADP-glucose with manganese1 Publication
  3. KM=652 µM for UDP-glucose with manganese1 Publication
  4. KM=606 µM for UDP-galactose with manganese1 Publication
  5. KM=714 µM for UDP-xylose with manganese1 Publication
  6. KM=107 µM for UDP-glucuronate with manganese1 Publication
  7. KM=108 µM for UDP-galacturonate with manganese1 Publication
  8. KM=416 µM for CDP-glucose with manganese1 Publication
  9. KM=795 µM for CDP-glycerol with manganese1 Publication
  10. KM=784 µM for GDP-glucose with manganese1 Publication
  11. KM=343 µM for GDP-alpha-L-fucose with manganese1 Publication
  12. KM=114 µM for FAD with cobalt1 Publication
  13. KM=120 µM for ADP-glucose with cobalt1 Publication
  14. KM=2550 µM for UDP-glucose with cobalt1 Publication
  15. KM=3661 µM for UDP-galactose with cobalt1 Publication
  16. KM=2354 µM for UDP-xylose with cobalt1 Publication
  17. KM=539 µM for UDP-glucuronate with cobalt1 Publication
  18. KM=759 µM for UDP-galacturonate with cobalt1 Publication
  19. KM=3703 µM for CDP-glucose with cobalt1 Publication
  20. KM=2031 µM for CDP-glycerol with cobalt1 Publication
  21. KM=2246 µM for GDP-glucose with cobalt1 Publication
  22. KM=991 µM for GDP-alpha-L-fucose with cobalt1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei109DihydroxyacetonePROSITE-ProRule annotation1
    Binding sitei114DihydroxyacetonePROSITE-ProRule annotation1
    Active sitei221Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation1
    Binding sitei486ATP; via carbonyl oxygenBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi401 – 404ATPBy similarity4
    Nucleotide bindingi446 – 447ATPBy similarity2
    Nucleotide bindingi494 – 495ATPBy similarity2
    Nucleotide bindingi556 – 558ATPBy similarity3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionKinase, Lyase, Multifunctional enzyme, Transferase
    LigandATP-binding, Cobalt, FAD, Flavoprotein, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15866
    ReactomeiR-RNO-70350 Fructose catabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Triokinase/FMN cyclase
    Alternative name(s):
    Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
    Including the following 2 domains:
    ATP-dependent dihydroxyacetone kinase (EC:2.7.1.28, EC:2.7.1.29)
    Short name:
    DHA kinase
    Alternative name(s):
    Glycerone kinase
    Triokinase
    Triose kinase
    FAD-AMP lyase (cyclizing) (EC:4.6.1.15)
    Alternative name(s):
    FAD-AMP lyase (cyclic FMN forming)
    FMN cyclase
    Gene namesi
    Name:Tkfc
    Synonyms:Dak
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 1

    Organism-specific databases

    RGDi1311026 Tkfc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001215271 – 578Triokinase/FMN cyclaseAdd BLAST578

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei511PhosphoserineCombined sources1
    Modified residuei545PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ4KLZ6
    PRIDEiQ4KLZ6

    PTM databases

    iPTMnetiQ4KLZ6
    PhosphoSitePlusiQ4KLZ6

    Expressioni

    Gene expression databases

    BgeeiENSRNOG00000020704
    GenevisibleiQ4KLZ6 RN

    Interactioni

    Subunit structurei

    Homodimer (By similarity). Interacts with IFIH1 (via the CARD domains), the interaction is inhibited by viral infection (By similarity).By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028102

    Structurei

    3D structure databases

    ProteinModelPortaliQ4KLZ6
    SMRiQ4KLZ6
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini9 – 336DhaKPROSITE-ProRule annotationAdd BLAST328
    Domaini372 – 571DhaLPROSITE-ProRule annotationAdd BLAST200

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni56 – 59Dihydroxyacetone bindingBy similarity4

    Domaini

    DhaK and DhaL domains have differential roles, individually DhaK is inactive and DhaL displays cyclase but not kinase activity.By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG2426 Eukaryota
    COG2376 LUCA
    GeneTreeiENSGT00390000015415
    HOGENOMiHOG000234158
    HOVERGENiHBG079502
    InParanoidiQ4KLZ6
    KOiK00863
    OMAiAWPNVAK
    OrthoDBiEOG091G0P13
    PhylomeDBiQ4KLZ6
    TreeFamiTF313821

    Family and domain databases

    Gene3Di1.25.40.340, 1 hit
    InterProiView protein in InterPro
    IPR012734 DhaK_ATP
    IPR004006 DhaK_dom
    IPR004007 DhaL_dom
    IPR036117 DhaL_dom_sf
    PfamiView protein in Pfam
    PF02733 Dak1, 1 hit
    PF02734 Dak2, 1 hit
    SMARTiView protein in SMART
    SM01120 Dak2, 1 hit
    SUPFAMiSSF101473 SSF101473, 1 hit
    TIGRFAMsiTIGR02361 dak_ATP, 1 hit
    PROSITEiView protein in PROSITE
    PS51481 DHAK, 1 hit
    PS51480 DHAL, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q4KLZ6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSKKMVNSV EGCAGDALAG FVACNPDLQL LQGYRVALRS DLDSLKGRVA
    60 70 80 90 100
    LLSGGGSGHE PAHAGFIGKG MLTGVIAGAV FASPAVGSIL AAIRAVAQAG
    110 120 130 140 150
    TAGTLLIVKN YTGDRLNFGL AMEQAKAEGI SVEMVVIEDD SAFTVLKKAG
    160 170 180 190 200
    RRGLCGTILI HKVAGALAEE GMGLEEITKK VSVIAKAIGT LGVSLSPCSV
    210 220 230 240 250
    PGTKPTFELA ADEMELGLGI HGEAGVRRIK LVPVDQIVTL MLDHMTDTSN
    260 270 280 290 300
    ISHVPVKSGS SVVLMVNNLG GLSFLELGII ADAAIRLLEG RGVKVARALV
    310 320 330 340 350
    GTFMSALEMR GVSLTLMLVD EPLLKLIDAE TNAKAWPHMS KVSVTGRNRI
    360 370 380 390 400
    RAAPTEPAEA PEATAAGGVA SKQMTLVLDR ISTTLIGLEE HLNALDRAAG
    410 420 430 440 450
    DGDCGSTHSR AAKAIQGWLK EGPTPASPAQ VLSKLSVLLL EKMGGSSGAL
    460 470 480 490 500
    YGLFLTAAAQ PLKANTDLPA WSAAMDAGLK AMQKYGKAAP GDRTMLDSLW
    510 520 530 540 550
    AAAQELQAWK SPGASLLPVL TKAVKSAEAA AEATKNMEAG AGRASYISSA
    560 570
    QLDQPDPGAV AAAAIFRAIL EVLQTKAA
    Length:578
    Mass (Da):59,444
    Last modified:August 2, 2005 - v1
    Checksum:iC343482447F9770B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BC098925 mRNA Translation: AAH98925.1
    RefSeqiNP_001034120.1, NM_001039031.1
    XP_006231118.1, XM_006231056.3
    UniGeneiRn.55630

    Genome annotation databases

    EnsembliENSRNOT00000028102; ENSRNOP00000028102; ENSRNOG00000020704
    GeneIDi361730
    KEGGirno:361730
    UCSCiRGD:1311026 rat

    Similar proteinsi

    Entry informationi

    Entry nameiTKFC_RAT
    AccessioniPrimary (citable) accession number: Q4KLZ6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: August 2, 2005
    Last modified: May 23, 2018
    This is version 96 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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