Reviewed, UniProtKB/Swiss-Prot Q4KLP0 (DHTK1_RAT)

Names and origin

Protein names

Recommended name:
    Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial
    EC=1.2.4.2
Alternative name(s):
    Dehydrogenase E1 and transketolase domain-containing protein 1

Gene names

Name:

Dhtkd1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate By similarity.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: EC thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 920		Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial		PRO_0000307939

Sequences

	Sequence		Length	Mass (Da)
	Q4KLP0-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: AA4A70E36AA41104	FASTA	920	102,642
	10 20 30 40 50 60 MASATVAAAG RALRRAVPLL RRSYQTERGV YGYRPRKAGS GEPRGDRARP SVDHGLARLV 70 80 90 100 110 120 TVYCEHGHKA AQINPLFPGQ ALLDTVPEIQ ALVQTLQGPF TTTGLLNMGK EEASLEEVLA 130 140 150 160 170 180 YLNHIYCGPI SIETAQLQSQ EEKDWFARRF EELKKETFTT EERKHLSKLL LESQEFDHFL 190 200 210 220 230 240 ATKFATVKRY GGEGAESMMG FFHELLKLSA YGGITDIIIG MPHRGRLNLL TGLLQLPPEL 250 260 270 280 290 300 MFRKMRGLSE FPENVAAIGD VLSHLTSSVD LDFGAHRPLH VTMLPNPSHL EAINPVAVGK 310 320 330 340 350 360 TRGRQQSQED GDYSPNGSAQ PGDKVICLQV HGDASFCGQG IVLETFTLSN LPHFRIGGSI 370 380 390 400 410 420 HLIVNNQLGY TTPAERGRSS LYSSDIGKLV GCAIIHVNGD SPEEVVRATR LAFEYQRQFR 430 440 450 460 470 480 KDVIIDLLCY RQWGHNELDE PFFTNPVMYK IIRARKSIPD TYAEHLIASG LMTQEEVSDI 490 500 510 520 530 540 KASYYAKLNG HLANVAHYSP PAPHLQARWQ GLVQPAACVT TWDTGVPLEL LRFVGVKSVE 550 560 570 580 590 600 VPEELQLHSH LLKMYVQSRM EKVKNGTNLD WATAETLALG SLLAQGFNVR LSGQDVGRGT 610 620 630 640 650 660 FSQRHAMVVC QNTDDVYIPL NHMDPNQKGF LEVSNSPLSE EAVLGFEYGM SIESPKLLPL 670 680 690 700 710 720 WEAQFGDFFN GAQIIFDTFI SGGEAKWLLQ SGLVILLPHG YDGAGPDHSS CRIERFLQMC 730 740 750 760 770 780 DSAEEGVDSD TVNMFVVHPT TPAQYFHLLR RQMMRNFRKP LIVASPKMLL RYPVAVSTLE 790 800 810 820 830 840 EMAPGTAFKP VIGDSSVDPK NVKTLIFCSG KHFYALLKQR ESLGAKKRDF AIIRLEELCP 850 860 870 880 890 900 FPLDSLQQEM GKYKHVQDII WSQEEPQNMG PWSFVYPRFE KQLACKLRLV SRPPLPAPAV 910 920 GIGTVHQQQH EAILFKTFTS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC099075 mRNA. Translation: AAH99075.1.
IPI	IPI00364925.
RefSeq	NP_001020891.1.
UniGene	Rn.13539
3D structure databases
SMR	Q4KLP0. Positions 55-919.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KLP0.
Genome annotation databases
Ensembl	ENSRNOT00000065978; ENSRNOP00000059952; ENSRNOG00000023587; Rattus norvegicus. [Genome view]
GeneID	361272.
KEGG	rno:361272.
Organism-specific databases
CTD	361272.
RGD	1308092. Dhtkd1.
Phylogenomic databases
eggNOG	roNOG08803.
HOVERGEN	Q4KLP0.
Enzyme and pathway databases
BRENDA	1.2.4.2. 248.
Gene expression databases
Genevestigator	Q4KLP0.
Family and domain databases
InterPro	IPR011603. 2oxoglutarate_DH_E1. IPR001017. DH_E1. IPR005475. Transketolase-like_Pyr-bd. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	675775.

Entry information

Entry name

DHTK1_RAT

Accession

Primary (citable) accession number: Q4KLP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)