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Protein

Ribonucleoside-diphosphate reductase subunit M2

Gene

Rrm2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity). Inhibits Wnt signaling (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Iron 1PROSITE-ProRule annotation1
Metal bindingi170Iron 1PROSITE-ProRule annotation1
Metal bindingi170Iron 2By similarity1
Metal bindingi173Iron 1PROSITE-ProRule annotation1
Active sitei177PROSITE-ProRule annotation1
Metal bindingi233Iron 2By similarity1
Metal bindingi267Iron 1PROSITE-ProRule annotation1
Metal bindingi267Iron 2By similarity1
Metal bindingi270Iron 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • deoxyribonucleotide biosynthetic process Source: UniProtKB
  • DNA replication Source: UniProtKB-UniPathway
  • mitotic cell cycle Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • pyrimidine nucleobase metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-113510. E2F mediated regulation of DNA replication.
R-RNO-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-RNO-69205. G1/S-Specific Transcription.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit M2 (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small chain
Ribonucleotide reductase small subunit
Gene namesi
Name:Rrm2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1598310. Rrm2.

Subcellular locationi

GO - Cellular componenti

  • nuclear envelope Source: RGD
  • ribonucleoside-diphosphate reductase complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001904511 – 390Ribonucleoside-diphosphate reductase subunit M2Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphoserineBy similarity1
Modified residuei33PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ4KLN6.
PRIDEiQ4KLN6.

PTM databases

iPTMnetiQ4KLN6.
PhosphoSitePlusiQ4KLN6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000023272.
GenevisibleiQ4KLN6. RN.

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000037227.

Structurei

3D structure databases

ProteinModelPortaliQ4KLN6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1567. Eukaryota.
COG0208. LUCA.
GeneTreeiENSGT00390000013305.
HOGENOMiHOG000255975.
HOVERGENiHBG001647.
InParanoidiQ4KLN6.
KOiK10808.
OMAiPIRFHEI.
OrthoDBiEOG091G0AZQ.
PhylomeDBiQ4KLN6.
TreeFamiTF300465.

Family and domain databases

CDDicd01049. RNRR2. 1 hit.
Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR033909. RNR_small.
IPR030475. RNR_small_AS.
IPR000358. RNR_small_fam.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KLN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVRAPLAT IADQQQLHLS PLKRLSLADK ENTPPTLSSA RVLASKAARR
60 70 80 90 100
IFQDSAELES KAPTKPSIEE EPLLRENPRR FVVFPIEYHD IWQMYKKAEA
110 120 130 140 150
SFWTAEEVDL SKDIQHWEAL KPDERHFISH VLAFFAASDG IVNENLVERF
160 170 180 190 200
SQEVQVTEAR CFYGFQIAME NIHSEMYSLL IDTYIKDSKE REYLFNAIET
210 220 230 240 250
MPCVKKKADW ALRWIGDKEA TYGERVVAFA AVEGIFFSGS FASIFWLKKR
260 270 280 290 300
GLMPGLTFSN ELISRDEGLH CDFACLMFKH LVHKPSEQRV KEIITNSVRI
310 320 330 340 350
EQEFLTEALP VKLIGMNCTL MKQYIEFVAD RLMLELGFNK IFKVENPFDF
360 370 380 390
MENISLEGKT NFFEKRVGEY QRMGVMSNST ENSFTLDADF
Length:390
Mass (Da):45,039
Last modified:August 2, 2005 - v1
Checksum:iA1BD4EABB7920F69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099082 mRNA. Translation: AAH99082.1.
RefSeqiNP_001020911.1. NM_001025740.1.
UniGeneiRn.144946.
Rn.160628.

Genome annotation databases

EnsembliENSRNOT00000091551; ENSRNOP00000073334; ENSRNOG00000054286.
GeneIDi362720.
KEGGirno:362720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099082 mRNA. Translation: AAH99082.1.
RefSeqiNP_001020911.1. NM_001025740.1.
UniGeneiRn.144946.
Rn.160628.

3D structure databases

ProteinModelPortaliQ4KLN6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000037227.

PTM databases

iPTMnetiQ4KLN6.
PhosphoSitePlusiQ4KLN6.

Proteomic databases

PaxDbiQ4KLN6.
PRIDEiQ4KLN6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000091551; ENSRNOP00000073334; ENSRNOG00000054286.
GeneIDi362720.
KEGGirno:362720.

Organism-specific databases

CTDi6241.
RGDi1598310. Rrm2.

Phylogenomic databases

eggNOGiKOG1567. Eukaryota.
COG0208. LUCA.
GeneTreeiENSGT00390000013305.
HOGENOMiHOG000255975.
HOVERGENiHBG001647.
InParanoidiQ4KLN6.
KOiK10808.
OMAiPIRFHEI.
OrthoDBiEOG091G0AZQ.
PhylomeDBiQ4KLN6.
TreeFamiTF300465.

Enzyme and pathway databases

UniPathwayiUPA00326.
ReactomeiR-RNO-113510. E2F mediated regulation of DNA replication.
R-RNO-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-RNO-69205. G1/S-Specific Transcription.

Miscellaneous databases

PROiQ4KLN6.

Gene expression databases

BgeeiENSRNOG00000023272.
GenevisibleiQ4KLN6. RN.

Family and domain databases

CDDicd01049. RNRR2. 1 hit.
Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR033909. RNR_small.
IPR030475. RNR_small_AS.
IPR000358. RNR_small_fam.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR2_RAT
AccessioniPrimary (citable) accession number: Q4KLN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: August 2, 2005
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2 (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.