Ribonucleoside-diphosphate reductase subunit M2

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Q4KLN6 (RIR2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase subunit M2
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small chain
Ribonucleotide reductase small subunit

Gene names

Name:

Rrm2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity. Inhibits Wnt signaling By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H₂O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling By similarity.
Miscellaneous	Two distinct regulatory sites have been defined: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2 By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro deoxyribonucleotide biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB mitotic cell cycle Inferred from expression pattern PubMed 6388757. Source: RGD positive regulation of cell proliferation Inferred from mutant phenotype PubMed 17404105. Source: RGD pyrimidine nucleobase metabolic process Inferred from expression pattern PubMed 10204803. Source: RGD
Cellular_component	nuclear envelope Inferred from direct assay PubMed 2205248. Source: RGD ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from sequence or structural similarity. Source: UniProtKB transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 390

390

Ribonucleoside-diphosphate reductase subunit M2

PRO_0000190451

Sites

Active site

177

By similarity

Metal binding

139

Iron 1 By similarity

Metal binding

170

Iron 1 By similarity

Metal binding

170

Iron 2 By similarity

Metal binding

173

Iron 1 By similarity

Metal binding

233

Iron 2 By similarity

Metal binding

267

Iron 1 By similarity

Metal binding

267

Iron 2 By similarity

Metal binding

270

Iron 2 By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q4KLN6 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: A1BD4EABB7920F69
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FASTA

390

45,039

        10         20         30         40         50         60 
MLSVRAPLAT IADQQQLHLS PLKRLSLADK ENTPPTLSSA RVLASKAARR IFQDSAELES 

        70         80         90        100        110        120 
KAPTKPSIEE EPLLRENPRR FVVFPIEYHD IWQMYKKAEA SFWTAEEVDL SKDIQHWEAL 

       130        140        150        160        170        180 
KPDERHFISH VLAFFAASDG IVNENLVERF SQEVQVTEAR CFYGFQIAME NIHSEMYSLL 

       190        200        210        220        230        240 
IDTYIKDSKE REYLFNAIET MPCVKKKADW ALRWIGDKEA TYGERVVAFA AVEGIFFSGS 

       250        260        270        280        290        300 
FASIFWLKKR GLMPGLTFSN ELISRDEGLH CDFACLMFKH LVHKPSEQRV KEIITNSVRI 

       310        320        330        340        350        360 
EQEFLTEALP VKLIGMNCTL MKQYIEFVAD RLMLELGFNK IFKVENPFDF MENISLEGKT 

       370        380        390 
NFFEKRVGEY QRMGVMSNST ENSFTLDADF

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References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC099082 mRNA. Translation: AAH99082.1.
IPI	IPI00365596.
RefSeq	NP_001020911.1. NM_001025740.1.
UniGene	Rn.144946.
3D structure databases
ProteinModelPortal	Q4KLN6.
SMR	Q4KLN6. Positions 7-353.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000037227.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000037387; ENSRNOP00000037227; ENSRNOG00000023272.
GeneID	362720.
KEGG	rno:362720.
Organism-specific databases
CTD	6241.
RGD	1598310. Rrm2.
Phylogenomic databases
eggNOG	COG0208.
GeneTree	ENSGT00390000013305.
HOGENOM	HOG000255975.
HOVERGEN	HBG001647.
InParanoid	Q4KLN6.
KO	K10808.
OrthoDB	EOG4BCDN7.
Enzyme and pathway databases
UniPathway	UPA00326.
Gene expression databases
Genevestigator	Q4KLN6.
GermOnline	ENSRNOG00000008450. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL3941.
NextBio	680993.

Entry information

Entry name

RIR2_RAT

Accession

Primary (citable) accession number: Q4KLN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 2, 2005
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents