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Q4KLM6

- P3H2_RAT

UniProt

Q4KLM6 - P3H2_RAT

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Protein

Prolyl 3-hydroxylase 2

Gene

Leprel1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V (By similarity). Catalyzes the 3-hydroxyproline modification in -Xaa-Pro-Gly-sequences in collagens, especially alpha-1 type II and alpha-2 type V.By similarity1 Publication

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi575 – 5751Iron
Metal bindingi577 – 5771Iron
Metal bindingi647 – 6471Iron
Active sitei657 – 6571By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. L-ascorbic acid binding Source: UniProtKB-KW
  3. procollagen-proline 3-dioxygenase activity Source: UniProtKB

GO - Biological processi

  1. collagen metabolic process Source: UniProtKB
  2. negative regulation of cell proliferation Source: UniProtKB
  3. peptidyl-proline hydroxylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiREACT_198584. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 2 (EC:1.14.11.7)
Alternative name(s):
Leprecan-like protein 1
Gene namesi
Name:Leprel1
Synonyms:P3h2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1304568. Leprel1.

Subcellular locationi

GO - Cellular componenti

  1. basement membrane Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB
  3. Golgi apparatus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 703682Prolyl 3-hydroxylase 2PRO_0000240358Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi455 – 4551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ4KLM6.
PRIDEiQ4KLM6.

PTM databases

PhosphoSiteiQ4KLM6.

Expressioni

Gene expression databases

ExpressionAtlasiQ4KLM6. baseline.
GenevestigatoriQ4KLM6.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002639.

Structurei

3D structure databases

ProteinModelPortaliQ4KLM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati42 – 7534TPR 1Add
BLAST
Repeati144 – 17734TPR 2Add
BLAST
Repeati205 – 23834TPR 3Add
BLAST
Repeati301 – 33434TPR 4Add
BLAST
Domaini552 – 666115Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi700 – 7034Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the leprecan family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.Curated

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiNOG269251.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ4KLM6.
PhylomeDBiQ4KLM6.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
IPR013105. TPR_2.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
PF07719. TPR_2. 2 hits.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4KLM6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRESTWVSLL LLLLLPAPQR GGPQDGRGSP EPEPERGPLQ PFDLLYASGV
60 70 80 90 100
AAYYSGDYEG AVRDLEAALR SHRRLRDIRT RCARHCAARR PLAPPGAGPG
110 120 130 140 150
AELPFFRAVL ERARCSRSCQ SQRLGGPASR HRVSEDVRSD FQRRVPYNYL
160 170 180 190 200
QRAYIKLNQL DKAMEAAHTF FMANPEHMEM QQNIEDYKAT ARVEAPLVDR
210 220 230 240 250
EAKPHLESYN AGVKHYEADD FEAAIKYFEQ ALREYFNEDM VCRALCEGPQ
260 270 280 290 300
RFEEYEYLGS KGSLYEAIAD HYMQVLVCQH ECVRELATRP GRLSPIENFL
310 320 330 340 350
PLHYDYLQFA YYRVGEYVKA LECAKAYLMF HPDDQDVLDN VDFYESLLDD
360 370 380 390 400
STDPASIEAR EDLTAFVKRH KLEAELIKSA AEGLGFSYSE PNYWISYGGR
410 420 430 440 450
QDENRVPSGV NMDGAEVHGL SMGKKSPPKI GRDLREGGPL LYENITFVYN
460 470 480 490 500
SEQLNGTQRV LLDNVLSEEQ CRELHSVASG IMLVGDGYRG KTSPHTPNEK
510 520 530 540 550
FEGATVLKAL KFGYEGRVPL KSARLFYDIS EKARKIVESY FMLNSTLYFS
560 570 580 590 600
YTHMVCRTAL SGQQDRRNDL SHPIHADNCL LDPEANECWK EPPAYTFRDY
610 620 630 640 650
SALLYMNDDF EGGEFIFTEM DAKTVTASIK PKCGRMISFS SGGENPHGVK
660 670 680 690 700
AVTRGQRCAV ALWFTLDPLY RELERIQADE VIAILDQEQH GKHGLNINPK

DEL
Length:703
Mass (Da):79,833
Last modified:August 2, 2005 - v1
Checksum:iEFE57CEF5F209B63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099107 mRNA. Translation: AAH99107.1.
RefSeqiNP_001020798.1. NM_001025627.1.
UniGeneiRn.213595.

Genome annotation databases

GeneIDi288016.
KEGGirno:288016.
UCSCiRGD:1304568. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099107 mRNA. Translation: AAH99107.1 .
RefSeqi NP_001020798.1. NM_001025627.1.
UniGenei Rn.213595.

3D structure databases

ProteinModelPortali Q4KLM6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000002639.

PTM databases

PhosphoSitei Q4KLM6.

Proteomic databases

PaxDbi Q4KLM6.
PRIDEi Q4KLM6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 288016.
KEGGi rno:288016.
UCSCi RGD:1304568. rat.

Organism-specific databases

CTDi 55214.
RGDi 1304568. Leprel1.

Phylogenomic databases

eggNOGi NOG269251.
HOGENOMi HOG000231087.
HOVERGENi HBG053224.
InParanoidi Q4KLM6.
PhylomeDBi Q4KLM6.

Enzyme and pathway databases

Reactomei REACT_198584. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

NextBioi 627416.
PROi Q4KLM6.

Gene expression databases

ExpressionAtlasi Q4KLM6. baseline.
Genevestigatori Q4KLM6.

Family and domain databases

Gene3Di 1.25.40.10. 3 hits.
InterProi IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
IPR013105. TPR_2.
[Graphical view ]
Pfami PF13640. 2OG-FeII_Oxy_3. 1 hit.
PF07719. TPR_2. 2 hits.
[Graphical view ]
SMARTi SM00702. P4Hc. 1 hit.
[Graphical view ]
PROSITEi PS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  2. "A role for prolyl 3-hydroxylase 2 in post-translational modification of fibril-forming collagens."
    Fernandes R.J., Farnand A.W., Traeger G.R., Weis M.A., Eyre D.R.
    J. Biol. Chem. 286:30662-30669(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS PROLYL-3-HYDROXYLASE.

Entry informationi

Entry nameiP3H2_RAT
AccessioniPrimary (citable) accession number: Q4KLM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: August 2, 2005
Last modified: November 26, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3