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Protein

Prolyl 3-hydroxylase 2

Gene

P3h2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V (By similarity). Catalyzes the 3-hydroxyproline modification in -Xaa-Pro-Gly-sequences in collagens, especially alpha-1 type II and alpha-2 type V.By similarity1 Publication

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi575Iron1
Metal bindingi577Iron1
Metal bindingi647Iron1
Active sitei657By similarity1

GO - Molecular functioni

GO - Biological processi

  • collagen metabolic process Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • peptidyl-proline hydroxylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 2Imported (EC:1.14.11.7)
Alternative name(s):
Leprecan-like protein 1By similarity
Gene namesi
Name:P3h2Imported
Synonyms:Leprel1By similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1304568. P3h2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000024035822 – 703Prolyl 3-hydroxylase 2Add BLAST682

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi444N-linked (GlcNAc...)Sequence analysis1
Glycosylationi455N-linked (GlcNAc...)Sequence analysis1
Glycosylationi544N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ4KLM6.
PRIDEiQ4KLM6.

PTM databases

iPTMnetiQ4KLM6.
PhosphoSitePlusiQ4KLM6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001925.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002639.

Structurei

3D structure databases

ProteinModelPortaliQ4KLM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati42 – 75TPR 1Add BLAST34
Repeati144 – 177TPR 2Add BLAST34
Repeati205 – 238TPR 3Add BLAST34
Repeati301 – 334TPR 4Add BLAST34
Domaini552 – 666Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST115

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi700 – 703Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the leprecan family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.Curated

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG4459. Eukaryota.
ENOG410XPXF. LUCA.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ4KLM6.
PhylomeDBiQ4KLM6.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4KLM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRESTWVSLL LLLLLPAPQR GGPQDGRGSP EPEPERGPLQ PFDLLYASGV
60 70 80 90 100
AAYYSGDYEG AVRDLEAALR SHRRLRDIRT RCARHCAARR PLAPPGAGPG
110 120 130 140 150
AELPFFRAVL ERARCSRSCQ SQRLGGPASR HRVSEDVRSD FQRRVPYNYL
160 170 180 190 200
QRAYIKLNQL DKAMEAAHTF FMANPEHMEM QQNIEDYKAT ARVEAPLVDR
210 220 230 240 250
EAKPHLESYN AGVKHYEADD FEAAIKYFEQ ALREYFNEDM VCRALCEGPQ
260 270 280 290 300
RFEEYEYLGS KGSLYEAIAD HYMQVLVCQH ECVRELATRP GRLSPIENFL
310 320 330 340 350
PLHYDYLQFA YYRVGEYVKA LECAKAYLMF HPDDQDVLDN VDFYESLLDD
360 370 380 390 400
STDPASIEAR EDLTAFVKRH KLEAELIKSA AEGLGFSYSE PNYWISYGGR
410 420 430 440 450
QDENRVPSGV NMDGAEVHGL SMGKKSPPKI GRDLREGGPL LYENITFVYN
460 470 480 490 500
SEQLNGTQRV LLDNVLSEEQ CRELHSVASG IMLVGDGYRG KTSPHTPNEK
510 520 530 540 550
FEGATVLKAL KFGYEGRVPL KSARLFYDIS EKARKIVESY FMLNSTLYFS
560 570 580 590 600
YTHMVCRTAL SGQQDRRNDL SHPIHADNCL LDPEANECWK EPPAYTFRDY
610 620 630 640 650
SALLYMNDDF EGGEFIFTEM DAKTVTASIK PKCGRMISFS SGGENPHGVK
660 670 680 690 700
AVTRGQRCAV ALWFTLDPLY RELERIQADE VIAILDQEQH GKHGLNINPK

DEL
Length:703
Mass (Da):79,833
Last modified:August 2, 2005 - v1
Checksum:iEFE57CEF5F209B63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099107 mRNA. Translation: AAH99107.1.
RefSeqiNP_001020798.1. NM_001025627.1.
UniGeneiRn.213595.

Genome annotation databases

GeneIDi288016.
KEGGirno:288016.
UCSCiRGD:1304568. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099107 mRNA. Translation: AAH99107.1.
RefSeqiNP_001020798.1. NM_001025627.1.
UniGeneiRn.213595.

3D structure databases

ProteinModelPortaliQ4KLM6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002639.

PTM databases

iPTMnetiQ4KLM6.
PhosphoSitePlusiQ4KLM6.

Proteomic databases

PaxDbiQ4KLM6.
PRIDEiQ4KLM6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi288016.
KEGGirno:288016.
UCSCiRGD:1304568. rat.

Organism-specific databases

CTDi55214.
RGDi1304568. P3h2.

Phylogenomic databases

eggNOGiKOG4459. Eukaryota.
ENOG410XPXF. LUCA.
HOGENOMiHOG000231087.
HOVERGENiHBG053224.
InParanoidiQ4KLM6.
PhylomeDBiQ4KLM6.

Miscellaneous databases

PROiQ4KLM6.

Gene expression databases

BgeeiENSRNOG00000001925.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP3H2_RAT
AccessioniPrimary (citable) accession number: Q4KLM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.