Reviewed, UniProtKB/Swiss-Prot Q4KLM6 (P3H2_RAT)

Names and origin

Protein names

Recommended name:
    Prolyl 3-hydroxylase 2
    EC=1.14.11.7
Alternative name(s):
    Leprecan-like protein 1

Gene names

Name:	Leprel1
Synonyms:	P3h2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V By similarity.
Catalytic activity	L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.
Cofactor	Iron By similarity. Ascorbate By similarity.
Subcellular location	Endoplasmic reticulum. Golgi apparatus By similarity.
Sequence similarities	Belongs to the leprecan family. Contains 1 Fe2OG dioxygenase domain. Contains 4 TPR repeats.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus
Domain	Repeat Signal TPR repeat
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW procollagen-proline 3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 703	682	Prolyl 3-hydroxylase 2		PRO_0000240358
Regions
	Repeat	42 – 75	34	TPR 1
	Repeat	144 – 177	34	TPR 2
	Repeat	205 – 238	34	TPR 3
	Repeat	301 – 334	34	TPR 4
	Domain	552 – 666	115	Fe2OG dioxygenase
	Motif	700 – 703	4	Prevents secretion from ER Potential
Sites
	Active site	657	1	By similarity
	Metal binding	575	1	Iron
	Metal binding	577	1	Iron
	Metal binding	647	1	Iron
Amino acid modifications
	Glycosylation	444	1	N-linked (GlcNAc...) Potential
	Glycosylation	455	1	N-linked (GlcNAc...) Potential
	Glycosylation	544	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4KLM6-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: EFE57CEF5F209B63	FASTA	703	79,833
	10 20 30 40 50 60 MRESTWVSLL LLLLLPAPQR GGPQDGRGSP EPEPERGPLQ PFDLLYASGV AAYYSGDYEG 70 80 90 100 110 120 AVRDLEAALR SHRRLRDIRT RCARHCAARR PLAPPGAGPG AELPFFRAVL ERARCSRSCQ 130 140 150 160 170 180 SQRLGGPASR HRVSEDVRSD FQRRVPYNYL QRAYIKLNQL DKAMEAAHTF FMANPEHMEM 190 200 210 220 230 240 QQNIEDYKAT ARVEAPLVDR EAKPHLESYN AGVKHYEADD FEAAIKYFEQ ALREYFNEDM 250 260 270 280 290 300 VCRALCEGPQ RFEEYEYLGS KGSLYEAIAD HYMQVLVCQH ECVRELATRP GRLSPIENFL 310 320 330 340 350 360 PLHYDYLQFA YYRVGEYVKA LECAKAYLMF HPDDQDVLDN VDFYESLLDD STDPASIEAR 370 380 390 400 410 420 EDLTAFVKRH KLEAELIKSA AEGLGFSYSE PNYWISYGGR QDENRVPSGV NMDGAEVHGL 430 440 450 460 470 480 SMGKKSPPKI GRDLREGGPL LYENITFVYN SEQLNGTQRV LLDNVLSEEQ CRELHSVASG 490 500 510 520 530 540 IMLVGDGYRG KTSPHTPNEK FEGATVLKAL KFGYEGRVPL KSARLFYDIS EKARKIVESY 550 560 570 580 590 600 FMLNSTLYFS YTHMVCRTAL SGQQDRRNDL SHPIHADNCL LDPEANECWK EPPAYTFRDY 610 620 630 640 650 660 SALLYMNDDF EGGEFIFTEM DAKTVTASIK PKCGRMISFS SGGENPHGVK AVTRGQRCAV 670 680 690 700 ALWFTLDPLY RELERIQADE VIAILDQEQH GKHGLNINPK DEL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC099107 mRNA. Translation: AAH99107.1.
IPI	IPI00561579.
RefSeq	NP_001020798.1.
UniGene	Rn.213595
3D structure databases
SMR	Q4KLM6. Positions 207-349, 452-669.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000002639; ENSRNOP00000002639; ENSRNOG00000001925; Rattus norvegicus. [Genome view]
GeneID	288016.
KEGG	rno:288016.
Organism-specific databases
CTD	288016.
RGD	1304568. Leprel1.
Phylogenomic databases
eggNOG	roNOG12503.
HOVERGEN	Q4KLM6.
InParanoid	Q4KLM6.
Enzyme and pathway databases
BRENDA	1.14.11.7. 248.
Gene expression databases
Genevestigator	Q4KLM6.
GermOnline	ENSRNOG00000001925. Rattus norvegicus.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. IPR006620. Pro_4_hyd_alph. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. PS51471. FE2OG_OXY. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	627416.

Entry information

Entry name

P3H2_RAT

Accession

Primary (citable) accession number: Q4KLM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)