Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q4KLM6 (P3H2_RAT)

Last modified February 9, 2010. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Prolyl 3-hydroxylase 2
    EC=1.14.11.7
Alternative name(s):
    Leprecan-like protein 1
Gene names
Name: Leprel1
Synonyms: P3h2
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length703 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V By similarity.

Catalytic activity

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor

Iron By similarity.

Ascorbate By similarity.

Subcellular location

Endoplasmic reticulum. Golgi apparatus By similarity.

Sequence similarities

Belongs to the leprecan family.

Contains 1 Fe2OG dioxygenase domain.

Contains 4 TPR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 703682Prolyl 3-hydroxylase 2
PRO_0000240358

Regions

Repeat42 – 7534TPR 1
Repeat144 – 17734TPR 2
Repeat205 – 23834TPR 3
Repeat301 – 33434TPR 4
Domain552 – 666115Fe2OG dioxygenase
Motif700 – 7034Prevents secretion from ER Potential

Sites

Active site6571 By similarity
Metal binding5751Iron
Metal binding5771Iron
Metal binding6471Iron

Amino acid modifications

Glycosylation4441N-linked (GlcNAc...) Potential
Glycosylation4551N-linked (GlcNAc...) Potential
Glycosylation5441N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q4KLM6-1 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: EFE57CEF5F209B63

FASTA70379,833
        10         20         30         40         50         60 
MRESTWVSLL LLLLLPAPQR GGPQDGRGSP EPEPERGPLQ PFDLLYASGV AAYYSGDYEG 

        70         80         90        100        110        120 
AVRDLEAALR SHRRLRDIRT RCARHCAARR PLAPPGAGPG AELPFFRAVL ERARCSRSCQ 

       130        140        150        160        170        180 
SQRLGGPASR HRVSEDVRSD FQRRVPYNYL QRAYIKLNQL DKAMEAAHTF FMANPEHMEM 

       190        200        210        220        230        240 
QQNIEDYKAT ARVEAPLVDR EAKPHLESYN AGVKHYEADD FEAAIKYFEQ ALREYFNEDM 

       250        260        270        280        290        300 
VCRALCEGPQ RFEEYEYLGS KGSLYEAIAD HYMQVLVCQH ECVRELATRP GRLSPIENFL 

       310        320        330        340        350        360 
PLHYDYLQFA YYRVGEYVKA LECAKAYLMF HPDDQDVLDN VDFYESLLDD STDPASIEAR 

       370        380        390        400        410        420 
EDLTAFVKRH KLEAELIKSA AEGLGFSYSE PNYWISYGGR QDENRVPSGV NMDGAEVHGL 

       430        440        450        460        470        480 
SMGKKSPPKI GRDLREGGPL LYENITFVYN SEQLNGTQRV LLDNVLSEEQ CRELHSVASG 

       490        500        510        520        530        540 
IMLVGDGYRG KTSPHTPNEK FEGATVLKAL KFGYEGRVPL KSARLFYDIS EKARKIVESY 

       550        560        570        580        590        600 
FMLNSTLYFS YTHMVCRTAL SGQQDRRNDL SHPIHADNCL LDPEANECWK EPPAYTFRDY 

       610        620        630        640        650        660 
SALLYMNDDF EGGEFIFTEM DAKTVTASIK PKCGRMISFS SGGENPHGVK AVTRGQRCAV 

       670        680        690        700 
ALWFTLDPLY RELERIQADE VIAILDQEQH GKHGLNINPK DEL 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC099107 mRNA. Translation: AAH99107.1.
IPIIPI00561579.
RefSeqNP_001020798.1.
UniGeneRn.213595

3D structure databases

SMRQ4KLM6. Positions 207-349, 452-669.
ModBaseSearch...

Genome annotation databases

EnsemblENSRNOT00000002639; ENSRNOP00000002639; ENSRNOG00000001925; Rattus norvegicus. [Genome view]
GeneID288016.
KEGGrno:288016.

Organism-specific databases

CTD288016.
RGD1304568. Leprel1.

Phylogenomic databases

eggNOGroNOG12503.
HOVERGENQ4KLM6.
InParanoidQ4KLM6.

Enzyme and pathway databases

BRENDA1.14.11.7. 248.

Gene expression databases

GenevestigatorQ4KLM6.
GermOnlineENSRNOG00000001925. Rattus norvegicus.

Family and domain databases

InterProIPR005123. Oxoglutarate/Fe-dep_oxygenase.
IPR006620. Pro_4_hyd_alph.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
PS50005. TPR. False negative.
PS50293. TPR_REGION. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio627416.

Entry information

Entry nameP3H2_RAT
AccessionPrimary (citable) accession number: Q4KLM6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: August 2, 2005
Last modified: February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents