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Protein

Kelch-like protein 25

Gene

Klhl25

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Keywordsi

Biological processTranslation regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-8951664 Neddylation
R-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 25
Alternative name(s):
Ectoderm-neural cortex protein 2
Short name:
ENC-2
Gene namesi
Name:Klhl25
Synonyms:Enc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1310815 Klhl25

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002723101 – 589Kelch-like protein 25Add BLAST589

Proteomic databases

PaxDbiQ4KLM4
PRIDEiQ4KLM4

PTM databases

PhosphoSitePlusiQ4KLM4

Expressioni

Gene expression databases

BgeeiENSRNOG00000010959
GenevisibleiQ4KLM4 RN

Interactioni

Subunit structurei

Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL25 and RBX1. Interacts with EIF4EBP1 (when hypophosphorylated) (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014553

Structurei

3D structure databases

ProteinModelPortaliQ4KLM4
SMRiQ4KLM4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 114BTBPROSITE-ProRule annotationAdd BLAST69
Domaini149 – 250BACKAdd BLAST102
Repeati296 – 340Kelch 1Add BLAST45
Repeati341 – 388Kelch 2Add BLAST48
Repeati389 – 444Kelch 3Add BLAST56
Repeati446 – 492Kelch 4Add BLAST47
Repeati493 – 538Kelch 5Add BLAST46
Repeati539 – 585Kelch 6Add BLAST47

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441 Eukaryota
ENOG410XNX8 LUCA
GeneTreeiENSGT00910000143979
HOGENOMiHOG000232171
HOVERGENiHBG000905
InParanoidiQ4KLM4
KOiK10462
OMAiTSDTWNC
OrthoDBiEOG091G03JU
PhylomeDBiQ4KLM4
TreeFamiTF329218

Family and domain databases

Gene3Di2.120.10.80, 1 hit
InterProiView protein in InterPro
IPR011705 BACK
IPR017096 BTB-kelch_protein
IPR000210 BTB/POZ_dom
IPR015915 Kelch-typ_b-propeller
IPR006652 Kelch_1
IPR030565 KLHL25
IPR011333 SKP1/BTB/POZ_sf
PANTHERiPTHR24410:SF10 PTHR24410:SF10, 1 hit
PfamiView protein in Pfam
PF07707 BACK, 1 hit
PF00651 BTB, 1 hit
PF01344 Kelch_1, 4 hits
PIRSFiPIRSF037037 Kelch-like_protein_gigaxonin, 1 hit
SMARTiView protein in SMART
SM00875 BACK, 1 hit
SM00225 BTB, 1 hit
SM00612 Kelch, 6 hits
SUPFAMiSSF117281 SSF117281, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit

Sequencei

Sequence statusi: Complete.

Q4KLM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSVHETRK SRSSTGSMNI SVFHKASHPD CVLAHLNTLR KHCMFTDVTL
60 70 80 90 100
WAGDRAFPCH RAVLAASSRY FEAMFSHGLR ESRDDTVNFQ DNLHPEVLEL
110 120 130 140 150
LLDFAYSSRI VINEENAESL LEAGDMLQFH DVRDAAAEFL EKNLSPSNCL
160 170 180 190 200
GMMVLSDAHQ CRRLYEFSCR MSLVHFETVR QSEDFNSLSR DTLLDLISRD
210 220 230 240 250
ELETEDERVV FEAILQWVKH DLEQRKVHLP LLLRNVRLAL LPSDCLKKAV
260 270 280 290 300
SGEALLMADE CTKLIIDEAF RCKTKILLND GVVTSPFARP RKAGHTLLIL
310 320 330 340 350
GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSASAIGC KVYVTGGRGS
360 370 380 390 400
ENGVSKDVWV YDTVHEEWSK AAPMLIARFG HGSAELENCL YVVGGHTSLA
410 420 430 440 450
GIFPASPSVS LKQVEKYDPG DNKWTMVAPM RDGVSNAAVV SAKLKLFVFG
460 470 480 490 500
GTSIHRDMVS KVQCFDPSDN RWTIKAECPQ PWRYTAAAVL GSQIFIMGGD
510 520 530 540 550
TEYTAASAYR FDCETNQWTR IGDMTAKRMS CHAVASGNKL YVVGGYFGTQ
560 570 580
RCKTLDCYDP TSDTWNCITS VPYSLIPTAF VSTWKHLPA
Length:589
Mass (Da):65,840
Last modified:August 2, 2005 - v1
Checksum:i98D72FC65AAAC97D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099111 mRNA Translation: AAH99111.1
RefSeqiNP_001034095.1, NM_001039006.1
XP_006229441.1, XM_006229379.2
XP_006229442.1, XM_006229380.2
XP_006229443.1, XM_006229381.3
XP_008757727.1, XM_008759505.2
UniGeneiRn.16545

Genome annotation databases

EnsembliENSRNOT00000014553; ENSRNOP00000014553; ENSRNOG00000010959
GeneIDi293023
KEGGirno:293023
UCSCiRGD:1310815 rat

Similar proteinsi

Entry informationi

Entry nameiKLH25_RAT
AccessioniPrimary (citable) accession number: Q4KLM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: August 2, 2005
Last modified: April 25, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health