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Protein

Transcription elongation factor A protein 1

Gene

Tcea1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri259 – 29941TFIIS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of exoribonuclease activity Source: RGD
  2. regulation of DNA-templated transcription, elongation Source: InterPro
  3. regulation of transcription from RNA polymerase II promoter Source: InterPro
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_273293. RNA Polymerase II Pre-transcription Events.
REACT_283793. Formation of RNA Pol II elongation complex.
REACT_297910. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_323975. Dual incision reaction in TC-NER.
REACT_343091. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 1
Alternative name(s):
Transcription elongation factor S-II protein 1
Gene namesi
Name:Tcea1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1309880. Tcea1.

Subcellular locationi

  1. Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Transcription elongation factor A protein 1PRO_0000121448Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei97 – 971PhosphoserineBy similarity
Modified residuei100 – 1001PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ4KLL0.
PRIDEiQ4KLL0.

PTM databases

PhosphoSiteiQ4KLL0.

Expressioni

Gene expression databases

GenevestigatoriQ4KLL0.

Interactioni

Subunit structurei

Interacts with EAF2. Associates with UBR5 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription by recruiting their promoters.By similarity

Protein-protein interaction databases

BioGridi263480. 2 interactions.
STRINGi10116.ENSRNOP00000010893.

Structurei

3D structure databases

ProteinModelPortaliQ4KLL0.
SMRiQ4KLL0. Positions 1-87, 252-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8078TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini140 – 256117TFIIS centralPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri259 – 29941TFIIS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1594.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiQ4KLL0.
KOiK03145.
OMAiRAMISFW.
OrthoDBiEOG7GQXWQ.
PhylomeDBiQ4KLL0.
TreeFamiTF314970.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KLL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDEVVRIAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV
60 70 80 90 100
NAIRKQSTDE EVTSLAKSLI KSWKKLLDGP STDKDSEEKK KEPAISSQNS
110 120 130 140 150
PEAREESSSS SHVSSRKDET NARDTYVSSF PRAPSTSDSV RLKCREMLAA
160 170 180 190 200
ALRTGDDYVA IGADEEELGS QIEEAIYQEI RNTDMKYKNR VRSRISNLKD
210 220 230 240 250
AKNPNLRKNV LCGNIPPDLF ARMTAEEMAS DELKEMRKNL TKEAIREHQM
260 270 280 290 300
AKTGGTQTDL FTCGKCKKKN CTYTQVQTRS ADEPMTTFVV CNECGNRWKF

C
Length:301
Mass (Da):33,893
Last modified:August 2, 2005 - v1
Checksum:iAD85071FBB589CB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099141 mRNA. Translation: AAH99141.1.
RefSeqiNP_001020906.1. NM_001025735.1.
XP_003752832.1. XM_003752784.3.
XP_006237878.1. XM_006237816.2.
XP_006251782.1. XM_006251720.2.
UniGeneiRn.6738.

Genome annotation databases

EnsembliENSRNOT00000010893; ENSRNOP00000010893; ENSRNOG00000022323.
GeneIDi362479.
498453.
KEGGirno:362479.
rno:498453.
UCSCiRGD:1309880. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC099141 mRNA. Translation: AAH99141.1.
RefSeqiNP_001020906.1. NM_001025735.1.
XP_003752832.1. XM_003752784.3.
XP_006237878.1. XM_006237816.2.
XP_006251782.1. XM_006251720.2.
UniGeneiRn.6738.

3D structure databases

ProteinModelPortaliQ4KLL0.
SMRiQ4KLL0. Positions 1-87, 252-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi263480. 2 interactions.
STRINGi10116.ENSRNOP00000010893.

PTM databases

PhosphoSiteiQ4KLL0.

Proteomic databases

PaxDbiQ4KLL0.
PRIDEiQ4KLL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010893; ENSRNOP00000010893; ENSRNOG00000022323.
GeneIDi362479.
498453.
KEGGirno:362479.
rno:498453.
UCSCiRGD:1309880. rat.

Organism-specific databases

CTDi6917.
RGDi1309880. Tcea1.

Phylogenomic databases

eggNOGiCOG1594.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiQ4KLL0.
KOiK03145.
OMAiRAMISFW.
OrthoDBiEOG7GQXWQ.
PhylomeDBiQ4KLL0.
TreeFamiTF314970.

Enzyme and pathway databases

ReactomeiREACT_273293. RNA Polymerase II Pre-transcription Events.
REACT_283793. Formation of RNA Pol II elongation complex.
REACT_297910. Formation of transcription-coupled NER (TC-NER) repair complex.
REACT_323975. Dual incision reaction in TC-NER.
REACT_343091. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

NextBioi680080.
PROiQ4KLL0.

Gene expression databases

GenevestigatoriQ4KLL0.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.

Entry informationi

Entry nameiTCEA1_RAT
AccessioniPrimary (citable) accession number: Q4KLL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 2, 2005
Last modified: April 29, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.