Q4KLL0 (TCEA1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor A protein 1

Alternative name(s):
Transcription elongation factor S-II protein 1

Gene names

Name:

Tcea1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus By similarity.
Subunit structure	Interacts with EAF2 By similarity. Associates with UBR5 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription by recruiting their promoters By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	S-II binds to RNA-polymerase II in the absence of transcription.
Sequence similarities	Belongs to the TFS-II family. Contains 1 TFIIS central domain. Contains 1 TFIIS N-terminal domain. Contains 1 TFIIS-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of DNA-dependent transcription, elongation Inferred from electronic annotation. Source: InterPro regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Transcription elongation factor A protein 1		PRO_0000121448
Regions
	Domain	3 – 80	78	TFIIS N-terminal
	Domain	140 – 256	117	TFIIS central
	Zinc finger	259 – 299	41	TFIIS-type
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	97	1	Phosphoserine By similarity
	Modified residue	100	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KLL0 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: AD85071FBB589CB5	FASTA	301	33,893
	10 20 30 40 50 60 MEDEVVRIAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV NAIRKQSTDE 70 80 90 100 110 120 EVTSLAKSLI KSWKKLLDGP STDKDSEEKK KEPAISSQNS PEAREESSSS SHVSSRKDET 130 140 150 160 170 180 NARDTYVSSF PRAPSTSDSV RLKCREMLAA ALRTGDDYVA IGADEEELGS QIEEAIYQEI 190 200 210 220 230 240 RNTDMKYKNR VRSRISNLKD AKNPNLRKNV LCGNIPPDLF ARMTAEEMAS DELKEMRKNL 250 260 270 280 290 300 TKEAIREHQM AKTGGTQTDL FTCGKCKKKN CTYTQVQTRS ADEPMTTFVV CNECGNRWKF C

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC099141 mRNA. Translation: AAH99141.1.
IPI	IPI00358677.
RefSeq	NP_001020906.1. NM_001025735.1. XP_002728223.1. XM_002728177.2. XP_003752832.1. XM_003752784.1.
UniGene	Rn.205137. Rn.6738.
3D structure databases
ProteinModelPortal	Q4KLL0.
SMR	Q4KLL0. Positions 1-87, 252-301.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000010893.
PTM databases
PhosphoSite	Q4KLL0.
Proteomic databases
PaxDb	Q4KLL0.
PRIDE	Q4KLL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000010893; ENSRNOP00000010893; ENSRNOG00000022323.
GeneID	362479. 498453.
KEGG	rno:362479. rno:498453.
UCSC	RGD:1309880. rat.
Organism-specific databases
CTD	6917.
RGD	1309880. Tcea1.
Phylogenomic databases
eggNOG	COG1594.
GeneTree	ENSGT00390000017794.
HOGENOM	HOG000195015.
HOVERGEN	HBG055022.
InParanoid	Q4KLL0.
KO	K03145.
OMA	MKNPNLR.
OrthoDB	EOG4SN1PH.
Gene expression databases
Genevestigator	Q4KLL0.
GermOnline	ENSRNOG00000005869. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.472.30. 1 hit. 1.20.930.10. 1 hit.
InterPro	IPR016492. TF_IIS-rel. IPR003617. TFIIS/CRSP70_N_sub. IPR003618. TFIIS_cen_dom. IPR017923. TFIIS_N. IPR017890. TFS2M. IPR006289. TFSII. IPR001222. Znf_TFIIS. [Graphical view]
Pfam	PF08711. Med26. 1 hit. PF01096. TFIIS_C. 1 hit. PF07500. TFIIS_M. 1 hit. [Graphical view]
PIRSF	PIRSF006704. TF_IIS. 1 hit.
SMART	SM00510. TFS2M. 1 hit. SM00509. TFS2N. 1 hit. SM00440. ZnF_C2C2. 1 hit. [Graphical view]
SUPFAM	SSF46942. TFIIS_centre. 1 hit. SSF47676. TFIIS_conserved. 1 hit.
TIGRFAMs	TIGR01385. TFSII. 1 hit.
PROSITE	PS51321. TFIIS_CENTRAL. 1 hit. PS51319. TFIIS_N. 1 hit. PS00466. ZF_TFIIS_1. 1 hit. PS51133. ZF_TFIIS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	680080.

Entry information

Entry name

TCEA1_RAT

Accession

Primary (citable) accession number: Q4KLL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 2, 2005
Last modified:	April 3, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program