Q4KKR7 (SYGA_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	PFL_0014

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas protegens

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Glycine--tRNA ligase alpha subunit HAMAP-Rule MF_00254		PRO_1000047470

Sequences

	Sequence		Length	Mass (Da)
	Q4KKR7 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 0BB3AFD6CCB2A600	FASTA	317	36,247
	10 20 30 40 50 60 MSQPTPAVRT FQDLILALQQ YWAEQGCVVL QPYDMEVGAG TFHTATFLRA IGPETWNAAY 70 80 90 100 110 120 VQPSRRPTDG RYGENPNRLQ HYYQFQVVLK PNPENFQELY LGSLKHVGLD PLVHDIRFVE 130 140 150 160 170 180 DNWESPTLGA WGLGWEVWLN GMEVTQFTYF QQAGGIECYP VTGEITYGLE RLAMYLQGVD 190 200 210 220 230 240 SVYDLVWADG PFGKVTYGDV FHQNEVEQST YNFEHANVEK LFELFDFYES EAKRLIELDQ 250 260 270 280 290 300 PLPLPSYEMV LKASHTFNLL DARRAISVTA RQQYILRVRT LARSVAQAYL LARAKLGFPM 310 ATPDLRDEVL AKLEAAQ

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY95432.1.
RefSeq	YP_257166.1. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4KKR7.
SMR	Q4KKR7. Positions 11-294.
ModBase	Search...
Protein-protein interaction databases
STRING	220664.PFL_0014.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY95432; AAY95432; PFL_0014.
GeneID	3480614.
KEGG	pfl:PFL_0014.
PATRIC	19869133. VBIPseFlu72549_0014.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0752.
HOGENOM	HOG000264291.
KO	K01878.
OMA	CVILQPY.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	PFLU220664:GIX8-14-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha.
InterPro	IPR006194. Gly-tRNA-synth_heterodimer. IPR002310. Gly-tRNA_ligase_asu. [Graphical view]
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. glyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_PSEF5

Accession

Primary (citable) accession number: Q4KKR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 2, 2005
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program