Q4KKQ4 (HEM6_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	PFL_0027

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000019486
Regions
	Region	49 – 58	10	Important for dimerization By similarity
	Region	109 – 111	3	Substrate binding By similarity
	Region	241 – 276	36	Important for dimerization By similarity
	Region	259 – 264	6	Substrate binding By similarity
Sites
	Active site	107	1	Proton donor By similarity
	Binding site	93	1	Substrate By similarity
	Site	176	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KKQ4 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 0A891DD13EB230F3	FASTA	305	34,437
	10 20 30 40 50 60 MTTRTEAVKA YLLDLQDRIC AALETEDGGA RFIEDAWTRP AGGGGRTRVI GDGAVIEKGG 70 80 90 100 110 120 VNFSHVFGSG LPPSASAHRP ELAGRGFEAL GVSLVIHPHN PHVPTSHANV RFFIAEKEGE 130 140 150 160 170 180 EPVWWFGGGF DLTPYYGNID DCVHWHRVAE RACAPFGADV YPRYKAWCDS YFHIKHRNEP 190 200 210 220 230 240 RGIGGLFFDD LNEWDFDTSF AFMRAIGDAY IEAYLPIVQR RKAAAYTAQQ REFQEFRRGR 250 260 270 280 290 300 YVEFNLVYDR GTLFGLQSGG RTESILMSLP PQVRWGYDWK AEPGSEEARL TEYFLQDRDW LAASN

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY95445.1.
RefSeq	YP_257179.1. NC_004129.6.
3D structure databases
HSSP	HSSP built from PDB template 2AEX based on UniProtKB P36551.
ProteinModelPortal	Q4KKQ4.
SMR	Q4KKQ4. Positions 1-301.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KKQ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3480627.
GenomeReviews	Gene locus PFL_0027 in contig CP000076_GR.
KEGG	pfl:PFL_0027.
NMPDR	fig|220664.3.peg.1492.
PATRIC	19869159. VBIPseFlu72549_0027.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	VKAYLLD.
PhylomeDB	Q4KKQ4.
ProtClustDB	PRK05330.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_0027-MONOMER.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidase_aer. IPR018375. Coprogen_oxidase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KO	K00228.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
SUPFAM	SSF102886. Coprogen_oxidas. 1 hit.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_PSEF5

Accession

Primary (citable) accession number: Q4KKQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program