Reviewed, UniProtKB/Swiss-Prot Q4KK14 (GSH1_PSEF5)

Names and origin

Protein names

Recommended name:
    Glutamate--cysteine ligase
    EC=6.3.2.2
Alternative name(s):
    Gamma-glutamylcysteine synthetase
    Gamma-ECS
      Short name=GCS

Gene names

Name:	gshA
Ordered Locus Names:	PFL_0273

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Glutamate--cysteine ligase HAMAP MF_00578		PRO_1000025180

Sequences

	Sequence		Length	Mass (Da)
	Q4KK14-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 3E9B99EFDAF10151	FASTA	532	59,803
	10 20 30 40 50 60 MKESNLSELL NRRLALLGER ANLSLLEQCL HGIERECLRV TGEGRLAQTP HPEELGSALT 70 80 90 100 110 120 NEQITTDYSE SLLEFITPAL KDPADTLASL DKIHRFAYSK LGNEYLWSPS MPCPLPAEED 130 140 150 160 170 180 IPIAYYGTSN IGQLKYVYRK GLALRYGKTM QCIAGIHYNF SLPEQLWPLL KQAEGFVGTD 190 200 210 220 230 240 RDYQSSAYIA LIRNFRRYSW LLMYLFGASP ALDAGFLRGR SHQLEQLDAE TLYLPYATSL 250 260 270 280 290 300 RMSDLGYQSK AQAGLTPCYN DLNSYTDSLR KAVATPYAPY VEVGTHKDGE WVQLNTNILQ 310 320 330 340 350 360 IENEYYSNIR PKRVTYTGER PIQALMARGI QYVEVRCLDI NPFLPLGIDI QEARFLDAFL 370 380 390 400 410 420 LYCALNDSPL FENNECGNAT SNFLAVVKEG RRPGLQLQRQ GQPVEMKEWA AQLLEQIAPL 430 440 450 460 470 480 AALLDQSHGS DVHSKALDAQ LAKVKDSSLT PSAQVLAAMS EHKESFTQFS LRQSQAHAEY 490 500 510 520 530 FRSQTLSKEE QAAFEEAARK SLEQQTELEQ NEVGDFDVFV GAYQASILAI SN

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY95684.1.
RefSeq	YP_257419.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3480866.
GenomeReviews	Gene locus PFL_0273 in contig CP000076_GR.
KEGG	pfl:PFL_0273.
NMPDR	fig|220664.3.peg.1759.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4KK14.
OMA	Q4KK14. DTLYLPY.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_0273-MON.
Family and domain databases
HAMAP	MF_00578. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_PSEF5

Accession

Primary (citable) accession number: Q4KK14

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)