Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acetyltransferase component of pyruvate dehydrogenase complex

Gene

aceF

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.UniRule annotation

Catalytic activityi

Acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine.UniRule annotation

Cofactori

(R)-lipoateUniRule annotationNote: Binds 3 lipoyl cofactors covalently.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferaseUniRule annotationImported, Transferase
Biological processGlycolysisUniRule annotation
LigandPyruvateImported

Enzyme and pathway databases

BioCyciPPRO220664:G1G4K-523-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyltransferase component of pyruvate dehydrogenase complexUniRule annotation (EC:2.3.1.12UniRule annotation)
Gene namesi
Name:aceFImported
Ordered Locus Names:PFL_0508Imported
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)Imported
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000008540 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Interactioni

Subunit structurei

Forms a 24-polypeptide structural core with octahedral symmetry.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_0508

Structurei

3D structure databases

ProteinModelPortaliQ4KJD1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 75Lipoyl-bindingInterPro annotationAdd BLAST74
Domaini115 – 189Lipoyl-bindingInterPro annotationAdd BLAST75
Domaini226 – 300Lipoyl-bindingInterPro annotationAdd BLAST75
Domaini348 – 385Peripheral subunit-binding (PSBD)InterPro annotationAdd BLAST38

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.UniRule annotation

Keywords - Domaini

LipoylUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4107QSN Bacteria
COG0508 LUCA
HOGENOMiHOG000281562
KOiK00627
OMAiTMEFESF
OrthoDBiPOG091H05OF

Family and domain databases

Gene3Di3.30.559.10, 1 hit
4.10.320.10, 1 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR001078 2-oxoacid_DH_actylTfrase
IPR006256 AcTrfase_Pyrv_DH_cplx
IPR000089 Biotin_lipoyl
IPR023213 CAT-like_dom_sf
IPR036625 E3-bd_dom_sf
IPR004167 PSBD
IPR011053 Single_hybrid_motif
PANTHERiPTHR43178:SF2 PTHR43178:SF2, 4 hits
PfamiView protein in Pfam
PF00198 2-oxoacid_dh, 1 hit
PF00364 Biotin_lipoyl, 3 hits
PF02817 E3_binding, 1 hit
SUPFAMiSSF47005 SSF47005, 1 hit
SSF51230 SSF51230, 3 hits
TIGRFAMsiTIGR01348 PDHac_trf_long, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 3 hits
PS00189 LIPOYL, 3 hits
PS51826 PSBD, 1 hit

Sequencei

Sequence statusi: Complete.

Q4KJD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELIRVPDI GSGEGEVIEL FVKVGDRIEA DQSILTLESD KASMEVPAPK
60 70 80 90 100
AGVIKSLKVK LGDRLKEGDE LLELEVEGAA AAAPAAAPAA KAEAKPAAAP
110 120 130 140 150
AAASAPAAAP AAASVQQVHV PDIGSSGKAQ IIEIQVKVGD KVEADQSLIT
160 170 180 190 200
LESDKASMEI PSPAAGVVKA ISVKLNDEVG TGDLILDLEV AGAAAPAAAP
210 220 230 240 250
AQAAAPAAAA APAPAAAPAA PVADSVQDIH VPDIGSAGKA KIIEVLVKAG
260 270 280 290 300
DSVEADQSLI TLESDKASME IPSPAAGVVE SVSIKLDDEV GTGDLILKLK
310 320 330 340 350
VKGAAPAAAP APAAAPSAPA PAAAAAPAAA APAAAPAAAP AKPGAKVHAG
360 370 380 390 400
PAVRQLAREF GVELSAVGAS GPHGRILKED VQVYVKAMMQ KAKEAPAAGG
410 420 430 440 450
ATGGAGIPPI PVVDFSRFGE IEEVPMTRLM QAGAANLHRS WLNVPHVTQF
460 470 480 490 500
DSADITELEA FRVAQKAVAE KAGVKLTILP LLLKSCAHLL KELPDFNSSL
510 520 530 540 550
APSGKAIIRK KYVNIGFAVD TPDGLLVPVI KNVDQKSLLQ LAAEAASLAE
560 570 580 590 600
KARTKKLSAD EMQGACFTIS SLGHIGGTGF TPIVNAPEVA ILGVSKATIQ
610 620 630 640
PVWDGKAFQP KLMLPLSLSY DHRVINGAAA ARFTKRLSDL LADIRTILL
Length:649
Mass (Da):65,685
Last modified:August 2, 2005 - v1
Checksum:i8089F14059C1D2BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA Translation: AAY95917.1
RefSeqiWP_011058881.1, NC_004129.6

Genome annotation databases

EnsemblBacteriaiAAY95917; AAY95917; PFL_0508
KEGGipfl:PFL_0508
PATRICifig|220664.5.peg.523

Entry informationi

Entry nameiQ4KJD1_PSEF5
AccessioniPrimary (citable) accession number: Q4KJD1
Entry historyiIntegrated into UniProtKB/TrEMBL: August 2, 2005
Last sequence update: August 2, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health