Q4KIP3 (T23O_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	PFL_0753

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Tryptophan 2,3-dioxygenase		PRO_0000360125
Regions
	Region	27 – 31	5	Substrate binding By similarity
	Region	52 – 56	5	Substrate binding By similarity
Sites
	Metal binding	241	1	Iron (heme axial ligand) By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	125	1	Heme By similarity
	Binding site	255	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KIP3 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 5914314FE92F8224	FASTA	283	32,342
	10 20 30 40 50 60 MSQCPFSSNP PAEWHNAELN FSDSMSYGDY LDLGRILSAQ HPLSPDHNEM LFIIQHQTSE 70 80 90 100 110 120 LWMKLMLHEL KAAREHVRQG QLPPAFKMLA RVSRIFDQLV HAWAVLATMT PSEYKSIRPY 130 140 150 160 170 180 LGQSSGFQSF QYREIEFILG NKSAALLRPH AHRPELLQSL EASIATPSMY DEAIALMARS 190 200 210 220 230 240 GLSIDPARLA LDSTTTTQHD PSVEAAWREV YANPSAYWDL YQLAEKFIDL EDSFRQWRFR 250 260 270 280 HVTTVERIIG FQPGTGGTEG VGYLRKMLDT VLFPELWRVR SSL

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY96155.1.
RefSeq	YP_257890.1. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4KIP3.
SMR	Q4KIP3. Positions 25-283.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KIP3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3481337.
GenomeReviews	Gene locus PFL_0753 in contig CP000076_GR.
KEGG	pfl:PFL_0753.
NMPDR	fig|220664.3.peg.2202.
PATRIC	19870683. VBIPseFlu72549_0773.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	QYREIEF.
ProtClustDB	CLSK867383.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_0753-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 2 hits. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_PSEF5

Accession

Primary (citable) accession number: Q4KIP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program