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Protein

Histidinol dehydrogenase

Gene

hisD

Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.UniRule annotation

Catalytic activityi

L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361NADUniRule annotation
Binding sitei197 – 1971NADUniRule annotation
Binding sitei220 – 2201NADUniRule annotation
Binding sitei243 – 2431SubstrateUniRule annotation
Metal bindingi265 – 2651ZincUniRule annotation
Binding sitei265 – 2651SubstrateUniRule annotation
Metal bindingi268 – 2681ZincUniRule annotation
Binding sitei268 – 2681SubstrateUniRule annotation
Active sitei333 – 3331Proton acceptorUniRule annotation
Active sitei334 – 3341Proton acceptorUniRule annotation
Binding sitei334 – 3341SubstrateUniRule annotation
Metal bindingi367 – 3671ZincUniRule annotation
Binding sitei367 – 3671SubstrateUniRule annotation
Binding sitei421 – 4211SubstrateUniRule annotation
Metal bindingi426 – 4261ZincUniRule annotation
Binding sitei426 – 4261SubstrateUniRule annotation

GO - Molecular functioni

  1. histidinol dehydrogenase activity Source: JCVI
  2. NAD binding Source: InterPro
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. histidine biosynthetic process Source: JCVI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciPFLU220664:GIX8-932-MONOMER.
UniPathwayiUPA00031; UER00014.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol dehydrogenaseUniRule annotation (EC:1.1.1.23UniRule annotation)
Short name:
HDHUniRule annotation
Gene namesi
Name:hisDUniRule annotation
Ordered Locus Names:PFL_0929
OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000008540 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Histidinol dehydrogenasePRO_0000135820Add
BLAST

2D gel databases

World-2DPAGE0008:Q4KI73.

Interactioni

Protein-protein interaction databases

STRINGi220664.PFL_0929.

Family & Domainsi

Sequence similaritiesi

Belongs to the histidinol dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0141.
HOGENOMiHOG000243914.
KOiK00013.
OMAiLSVQSFL.

Family and domain databases

HAMAPiMF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view]
PfamiPF00815. Histidinol_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000099. Histidinol_dh. 1 hit.
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00069. hisD. 1 hit.
PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KI73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPTAIRRL NAADPDFARH LDHLLSWESV SDDSVNQRVL DIIKAVRERG
60 70 80 90 100
DAALVEFTQR FDGLQVASMA DLILPRERLE LALTRITVAQ REALEKAAAR
110 120 130 140 150
VRSYHEKQKQ DSWTYTEADG TVLGQKVTPL DRAGLYVPGG KASYPSSVLM
160 170 180 190 200
NAIPAKVAGV TEVVMVVPTP RGEVNELVLA AACIAGVDRV FTIGGAQAVA
210 220 230 240 250
ALAYGTESVP QVDKVVGPGN IYVATAKRHV FGQVGIDMIA GPSEILVVCD
260 270 280 290 300
GQTDPDWIAM DLFSQAEHDE DAQAILVSPD AEFLDKVAAS IAKLLPTMER
310 320 330 340 350
AEIINTSING RGALIKVRDM QQAIEVANRI APEHLELSVA DPQAWLPQIR
360 370 380 390 400
HAGAIFMGRH TSEALGDYCA GPNHVLPTSG TARFSSPLGV YDFQKRSSII
410 420 430 440
FCSEQGASEL GRTASVLARG ESLTAHARSA EYRIVDDQDQ GQ
Length:442
Mass (Da):47,611
Last modified:January 10, 2006 - v2
Checksum:i3A870C6065ADEEEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY90216.2.
RefSeqiYP_258060.2. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY90216; AAY90216; PFL_0929.
KEGGipfl:PFL_0929.
PATRICi19871047. VBIPseFlu72549_0952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY90216.2.
RefSeqiYP_258060.2. NC_004129.6.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220664.PFL_0929.

2D gel databases

World-2DPAGE0008:Q4KI73.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY90216; AAY90216; PFL_0929.
KEGGipfl:PFL_0929.
PATRICi19871047. VBIPseFlu72549_0952.

Phylogenomic databases

eggNOGiCOG0141.
HOGENOMiHOG000243914.
KOiK00013.
OMAiLSVQSFL.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00014.
BioCyciPFLU220664:GIX8-932-MONOMER.

Family and domain databases

HAMAPiMF_01024. HisD.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR001692. Histidinol_DH_CS.
IPR022695. Histidinol_DH_monofunct.
IPR012131. Hstdl_DH.
[Graphical view]
PfamiPF00815. Histidinol_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000099. Histidinol_dh. 1 hit.
PRINTSiPR00083. HOLDHDRGNASE.
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR00069. hisD. 1 hit.
PROSITEiPS00611. HISOL_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf-5 / ATCC BAA-477.

Entry informationi

Entry nameiHISX_PSEF5
AccessioniPrimary (citable) accession number: Q4KI73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: April 1, 2015
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.