ID   HGD_PSEF5               Reviewed;         434 AA.
AC   Q4KI35;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   05-MAY-2009, entry version 24.
DE   RecName: Full=Homogentisate 1,2-dioxygenase;
DE            EC=1.13.11.5;
DE   AltName: Full=Homogentisicase;
DE   AltName: Full=Homogentisate oxygenase;
DE   AltName: Full=Homogentisic acid oxidase;
GN   Name=hmgA; OrderedLocusNames=PFL_0967;
OS   Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A.,
RA   Rosovitz M.J., Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W.,
RA   Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A.,
RA   Pierson L.S. III, Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas
RT   fluorescens Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- CATALYTIC ACTIVITY: Homogentisate + O(2) = 4-maleylacetoacetate.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Amino-acid degradation; L-phenylalanine degradation;
CC       acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
CC   -!- SIMILARITY: Belongs to the homogentisate dioxygenase family.
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DR   EMBL; CP000076; AAY90254.1; -; Genomic_DNA.
DR   RefSeq; YP_258098.1; -.
DR   GeneID; 3476741; -.
DR   GenomeReviews; CP000076_GR; PFL_0967.
DR   KEGG; pfl:PFL_0967; -.
DR   NMPDR; fig|220664.3.peg.2411; -.
DR   HOGENOM; Q4KI35; -.
DR   OMA; Q4KI35; HRNCMSE.
DR   BioCyc; PFLU220664:PFL_0967-MON; -.
DR   GO; GO:0004411; F:homogentisate 1,2-dioxygenase activity; IEA:HAMAP.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006572; P:tyrosine catabolic process; IEA:HAMAP.
DR   HAMAP; MF_00334; -; 1.
DR   InterPro; IPR005708; Homogentis_dOase.
DR   PANTHER; PTHR11056; Homogentis_dOase; 1.
DR   Pfam; PF04209; HgmA; 1.
DR   TIGRFAMs; TIGR01015; hmgA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Dioxygenase; Iron; Metal-binding; Oxidoreductase;
KW   Phenylalanine catabolism; Tyrosine catabolism.
FT   CHAIN         1    434       Homogentisate 1,2-dioxygenase.
FT                                /FTId=PRO_0000225791.
FT   METAL       332    332       Iron (By similarity).
FT   METAL       338    338       Iron (By similarity).
FT   METAL       368    368       Iron (By similarity).
SQ   SEQUENCE   434 AA;  47945 MW;  4C32086AE5EEE7A8 CRC64;
     MNLDSSASAL SYQSGFGNEF ASEALPGALP VGQNSPQKAP YGLYAELFSG TAFTMTRSEA
     RRTWMYRIQP SAKHPAFAKL ARQLAGGPLG EVTPNRLRWS PLQIPSEPTD FIDGLVAMVA
     NSAAQKPAGI SVYHYRANRS MERVFFNADG ELLLVPELGR LRIVTELGML DLEPLEIAVL
     PRGLKFRIEL LDPQARGYVA ENHGAPLRLP DLGPIGSNGL ANPRDFLAPV AHYEDLRQPT
     TLVQKYLGEL WGCELDHSPL NVVAWHGNNV PYKYDLRRFN TIGTVSFDHP DPSIFTVLTS
     PTSVPGLANL DFVIFPPRWM VAENTFRPPW FHRNLMNEFM GLIQGAYDAK AEGFLPGGAS
     LHSCMSAHGP DGETCTKAIN AQLQPAKIDN TMAFMFETSQ VLRPSQFALE CPELQNDYDA
     CWASLPVTFN PNRR
//