Q4KI35 (HGD_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	PFL_0967

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas protegens

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_0000225791
Sites
	Metal binding	332	1	Iron By similarity
	Metal binding	338	1	Iron By similarity
	Metal binding	368	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KI35 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 4C32086AE5EEE7A8	FASTA	434	47,945
	10 20 30 40 50 60 MNLDSSASAL SYQSGFGNEF ASEALPGALP VGQNSPQKAP YGLYAELFSG TAFTMTRSEA 70 80 90 100 110 120 RRTWMYRIQP SAKHPAFAKL ARQLAGGPLG EVTPNRLRWS PLQIPSEPTD FIDGLVAMVA 130 140 150 160 170 180 NSAAQKPAGI SVYHYRANRS MERVFFNADG ELLLVPELGR LRIVTELGML DLEPLEIAVL 190 200 210 220 230 240 PRGLKFRIEL LDPQARGYVA ENHGAPLRLP DLGPIGSNGL ANPRDFLAPV AHYEDLRQPT 250 260 270 280 290 300 TLVQKYLGEL WGCELDHSPL NVVAWHGNNV PYKYDLRRFN TIGTVSFDHP DPSIFTVLTS 310 320 330 340 350 360 PTSVPGLANL DFVIFPPRWM VAENTFRPPW FHRNLMNEFM GLIQGAYDAK AEGFLPGGAS 370 380 390 400 410 420 LHSCMSAHGP DGETCTKAIN AQLQPAKIDN TMAFMFETSQ VLRPSQFALE CPELQNDYDA 430 CWASLPVTFN PNRR

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY90254.1.
RefSeq	YP_258098.1. NC_004129.6.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	220664.PFL_0967.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY90254; AAY90254; PFL_0967.
GeneID	3476741.
KEGG	pfl:PFL_0967.
PATRIC	19871127. VBIPseFlu72549_0992.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	PFLU220664:GIX8-970-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEF5

Accession

Primary (citable) accession number: Q4KI35

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 2, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program