Reviewed, UniProtKB/Swiss-Prot Q4KI35 (HGD_PSEF5)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	PFL_0967

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_0000225791
Sites
	Metal binding	332	1	Iron By similarity
	Metal binding	338	1	Iron By similarity
	Metal binding	368	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KI35-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 4C32086AE5EEE7A8	FASTA	434	47,945
	10 20 30 40 50 60 MNLDSSASAL SYQSGFGNEF ASEALPGALP VGQNSPQKAP YGLYAELFSG TAFTMTRSEA 70 80 90 100 110 120 RRTWMYRIQP SAKHPAFAKL ARQLAGGPLG EVTPNRLRWS PLQIPSEPTD FIDGLVAMVA 130 140 150 160 170 180 NSAAQKPAGI SVYHYRANRS MERVFFNADG ELLLVPELGR LRIVTELGML DLEPLEIAVL 190 200 210 220 230 240 PRGLKFRIEL LDPQARGYVA ENHGAPLRLP DLGPIGSNGL ANPRDFLAPV AHYEDLRQPT 250 260 270 280 290 300 TLVQKYLGEL WGCELDHSPL NVVAWHGNNV PYKYDLRRFN TIGTVSFDHP DPSIFTVLTS 310 320 330 340 350 360 PTSVPGLANL DFVIFPPRWM VAENTFRPPW FHRNLMNEFM GLIQGAYDAK AEGFLPGGAS 370 380 390 400 410 420 LHSCMSAHGP DGETCTKAIN AQLQPAKIDN TMAFMFETSQ VLRPSQFALE CPELQNDYDA 430 CWASLPVTFN PNRR

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY90254.1.
RefSeq	YP_258098.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3476741.
GenomeReviews	Gene locus PFL_0967 in contig CP000076_GR.
KEGG	pfl:PFL_0967.
NMPDR	fig|220664.3.peg.2411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4KI35.
OMA	Q4KI35. HRNCMSE.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_0967-MON.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEF5

Accession

Primary (citable) accession number: Q4KI35

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 2, 2005
Last modified:	May 5, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)