Q4KHY5 (ALGL_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alginate lyase
EC=4.2.2.3

Alternative name(s):
Poly(beta-D-mannuronate) lyase
Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	PFL_1017

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond By similarity. HAMAP MF_00557
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Subcellular location	Periplasm Potential HAMAP MF_00557.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 371	345	Alginate lyase HAMAP MF_00557		PRO_1000061112

Sequences

	Sequence		Length	Mass (Da)
	Q4KHY5 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: C2E09D7BCB8BB119	FASTA	371	41,682
	10 20 30 40 50 60 MQSTDLKRLL IPSLLGLAIV TGSAQAAAPL RPPQGYYAPV DKFKSGDNSE GCDAMPAPYT 70 80 90 100 110 120 GALQFRSKYE GSDKARATLN VQSEQAFRDT TADITKIERG TSKRVMQFMR DGRPEQLDCT 130 140 150 160 170 180 LAWLSAWAQA DALMSKDFNH TGKSMRKWAL GSMASAYLRL KFSDSHPLAT HQEQAQKIEA 190 200 210 220 230 240 WFSKMADQVV SDWDNLPLDK TNNHSYWAAW SVMATAVATN RRDLFDWAVK EYKVGANQVD 250 260 270 280 290 300 ADGFLPNELK RQQRALAYHN YALPPLAMIA SFAQVNGVDL RQENHEALKR LGERVLAGVK 310 320 330 340 350 360 DPDTFEKKNG KQQDMTDLKV DSKFAWLEPY CSLYTCAPET LERKHKMQPF KTFRLGGDLT 370 KVYDPAHEKG S

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY90304.1.
RefSeq	YP_258148.1. NC_004129.6.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KHY5.
Protein family/group databases
CAZy	PL5. Polysaccharide Lyase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3475409.
GenomeReviews	Gene locus PFL_1017 in contig CP000076_GR.
KEGG	pfl:PFL_1017.
NMPDR	fig|220664.3.peg.2461.
PATRIC	19871229. VBIPseFlu72549_1043.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG42597.
HOGENOM	HBG336064.
OMA	AAWSVMA.
ProtClustDB	PRK00325.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_1017-MONOMER.
Family and domain databases
HAMAP	MF_00557. Alginate_lyase. [Tree]
InterPro	IPR022859. Alginate_lyase. IPR008397. Alginate_lyase_dom. IPR008929. Chondroitin_lyas. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
KO	K01729.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
SUPFAM	SSF48230. Chondroitin_lyas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGL_PSEF5

Accession

Primary (citable) accession number: Q4KHY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program