Q4KHU0 (RLMF_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	PFL_1062

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.
Sequence caution	The sequence AAY90349.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349929

Sequences

	Sequence		Length	Mass (Da)
	Q4KHU0 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: BC424027E6BB5C90	FASTA	341	37,775
	10 20 30 40 50 60 MTAPSTPKPS RKKPKPASPA KPATPREKAS LHPRNRHQGR YDFPQLIKST PELGQFVILN 70 80 90 100 110 120 PYGKESIDFA DPAAVRVFNR ALLKAFYGIA HWDIPADFLC PPVPGRADYV HFLADLLASH 130 140 150 160 170 180 NQGEIPRGAA VRVLDVGVGA NCIYPLIGYS DYRWQFLGSD IDSTALAAAK AIVQANDLNK 190 200 210 220 230 240 AISLRQQGNP KQILTGLLDS TERFDLTMCN PPFHASLDEA TRGSQRKWRA LGKADPKRKL 250 260 270 280 290 300 PVLNFGGQAR ELWCEGGEIR FVSQLISESA SVGQQVLWFS TLVSKASNLP AIESALKKAG 310 320 330 340 ALESQVVEMS QGQKQSRFVA WTFKDKAQQQ AWSQERWMRK N

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY90349.1. Different initiation.
RefSeq	YP_258193.2. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4KHU0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KHU0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3477473.
GenomeReviews	Gene locus PFL_1062 in contig CP000076_GR.
KEGG	pfl:PFL_1062.
NMPDR	fig|220664.3.peg.2506.
PATRIC	19871323. VBIPseFlu72549_1090.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_1062-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_PSEF5

Accession

Primary (citable) accession number: Q4KHU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program