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Q4KHS6 (PUR4_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:PFL_1076
OrganismPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]
Taxonomic identifier220664 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length1298 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12981298Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000264587

Regions

Domain1045 – 1298254Glutamine amidotransferase type-1
Nucleotide binding305 – 31612ATP Potential

Sites

Active site11381Nucleophile By similarity
Active site12631 By similarity
Active site12651 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4KHS6 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 1DB74DABC816D815

FASTA1,298140,509
        10         20         30         40         50         60 
MLILRGAPAL SAFRHSKLLE QLSQKVPAVS GLYAEFAHFA EVTGVLTGDE QQVLARLLKY 

        70         80         90        100        110        120 
GPSVPVQEPT GRLFLVLPRF GTISPWSSKA SDIARNCGLA KIQRLERGIA FYVAGEFSEA 

       130        140        150        160        170        180 
EAQLIADALH DRMTQIVVAN LEQAAGLFSH AEPKPLTAID ILGGGRAALE QANSELGLAL 

       190        200        210        220        230        240 
AEDEIDYLMN AFQGLKRNPH DIELMMFAQA NSEHCRHKIF NASWDIDGQS QEKSLFGMIK 

       250        260        270        280        290        300 
NTYQMHNEGV LSAYKDNAAV IVGSVAGRFY PNPETRQYGA VQEPVHILMK VETHNHPTAI 

       310        320        330        340        350        360 
APFPGASTGS GGEIRDEGAT GRGAKPKAGL TGFTVSNLQI PGFEQPWEVP YGKPERIVTA 

       370        380        390        400        410        420 
LDIMIEGPLG GAAFNNEFGR PALTGYFRTF EQSITTPRGD EVRGYHKPIM LAGGMGNIRD 

       430        440        450        460        470        480 
EHVQKGEITV GSKLIVLGGP AMLIGLGGGA ASSMATGTSS ADLDFASVQR ENPEMERRCQ 

       490        500        510        520        530        540 
EVIDRCWQLG DNNPISFIHD VGAGGLSNAF PELVNDGGRG GRFELRNIPN DEPGMAPHEI 

       550        560        570        580        590        600 
WSNESQERYV LAVGAADFER FKAICERERC PFAVVGEATA EPQLTVTDSH FGNSPVDMPL 

       610        620        630        640        650        660 
EVLLGKAPRM HRSAVRENEL GDDFDPSTLD IAESIERVLH HPAVASKSFL ITIGDRTITG 

       670        680        690        700        710        720 
LVARDQMVGP WQVPVADVAV TATSFDVYTG EAMAMGERTP LALLDAPASG RMAIGETLTN 

       730        740        750        760        770        780 
IAASRINKIS DIKLSANWMS AAGHPGEDAR LYDTVKAVGM ELCPELGITI PVGKDSMSMA 

       790        800        810        820        830        840 
TRWNEEGVDK SVTSPLSLIV TGFAPVADIR QTLTPQLRMD KGTTDLILID LGRGQNRMGA 

       850        860        870        880        890        900 
SILAQTHGKL GSQAPDVDDA EDLKAFFAVI QGLNADGHLL AYHDRSDGGL LVSAVEMAFA 

       910        920        930        940        950        960 
GHCGLNLSLD AVAENTAEIA AILFNEELGA VIQVRQDATP DVLAQFSAAG LGDCVAVIGQ 

       970        980        990       1000       1010       1020 
PINNGEVSIS YNGESVFTGQ RRLLQRQWAE TSYQIQRLRD NADCAEQEFD VVLEEDNPGL 

      1030       1040       1050       1060       1070       1080 
SVKLGFDVNQ DIAAPYIKKG VRPQVAVLRE QGVNGQVEMA AAFDRAGFNA IDVHMSDILA 

      1090       1100       1110       1120       1130       1140 
GRVDLNDFKG MVACGGFSYG DVLGAGEGWA KSALFNSRAR DAFQGFFERT DSFTLGVCNG 

      1150       1160       1170       1180       1190       1200 
CQMMSNLHEL IPGSEFWPHF VRNRSEQFEA RVAMVQVQES NSIFLQGMAG SRMPIAIAHG 

      1210       1220       1230       1240       1250       1260 
EGHAEFESEE ALLEADLSGC VALRFVDNHG KVTERYPANP NGSPRGITGL TSRDGRVTIM 

      1270       1280       1290 
MPHPERVFRA VQNSWRPDEW NEDGAWMRMF RNARVWVN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000076 Genomic DNA. Translation: AAY90363.1.
RefSeqYP_258207.1. NC_004129.6.

3D structure databases

HSSPHSSP built from PDB template 1VK3 based on UniProtKB Q9X0X3.
ProteinModelPortalQ4KHS6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ4KHS6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3477487.
GenomeReviewsGene locus PFL_1076 in contig CP000076_GR.
KEGGpfl:PFL_1076.
NMPDRfig|220664.3.peg.2520.
PATRIC19871353. VBIPseFlu72549_1105.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG335309.
OMAVKAVGME.
PhylomeDBQ4KHS6.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycPFLU220664:PFL_1076-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_PSEF5
AccessionPrimary (citable) accession number: Q4KHS6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: August 2, 2005
Last modified: January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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