Reviewed, UniProtKB/Swiss-Prot Q4KHJ1 (PLSB_PSEF5)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PFL_1161

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	834 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 834	834	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049445
Regions
	Motif	309 – 314	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q4KHJ1-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 81526C3E25F5A784	FASTA	834	94,862
	10 20 30 40 50 60 MTRSPIRRLV FGTLRRLLYL WVRSETINQS SFTLDLDRSR PVFYVLQNPS LTDLAVVDTE 70 80 90 100 110 120 CTKAGLPRPV LPVSVGNLLE PAAFFYLTPE PDWLGRQDKR GAPPTLTRLV SALTQNAAED 130 140 150 160 170 180 AQIIPVSVFW GQSPDSESSP WKLLFADSWA VTGRLRRLLS IMILGRKTRV QFSAPINLRE 190 200 210 220 230 240 LIEHNKGHER TVRMAQRILR VHFRNLKAAV IGPDISHRRN LVKGLLNQPL VKQAILDEAE 250 260 270 280 290 300 REKISPEKAK AQALRYGNEI ASDYTYTAIR FLEVVLSWFW NKIYDGIKVN HLEGVQKIAQ 310 320 330 340 350 360 GHEVIYVPCH RSHIDYLLLS YLLFRNGLTP PHIAAGINLN MPVIGGLLRR GGAFFMRRTF 370 380 390 400 410 420 KGNPLYTSVF NEYLHTLFTK GFPVEYFVEG GRSRTGRMLQ PKTGMLAITL RSFLRSSRMP 430 440 450 460 470 480 IVFVPVYIGY ERVLEGRTYL GELRGASKKK ESIFDIFKVI GALKQRFGQV AVNFGEPIKL 490 500 510 520 530 540 AEFLDQEQPD WRTQELGPQY RPAWLNETTN RLGERVAQHL NEAAAINPVN LVALALLSTT 550 560 570 580 590 600 RLALDDRAMA RVLDLYLALL RRVPYSPHTT LPEGDGRALI QHVKDMDLLA EQSDALGKIL 610 620 630 640 650 660 YLDEQNAVLM TYYRNNVLHI FALPALLASF FQSSSRMSRE QILRYTRALY PYLQSELFIR 670 680 690 700 710 720 WPLDELDAVV DQWLEAFVEQ GLLRFEKDLY QRPAPSSRHF VLLTLLSKSI AQTLQRFYMA 730 740 750 760 770 780 ISLLLNSGQN SISAEELEDL CTVMAQRLSI LHGLNAPEFF DKSLFRHFIQ TLLDQGVLRR 790 800 810 820 830 DEAGKLSYHE ALGELAEGAA KRVLPAEIRL SIRQVALHRS EDAADQLAAP AQND

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY90448.1.
RefSeq	YP_258292.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KHJ1.
Genome annotation databases
GeneID	3478978.
GenomeReviews	Gene locus PFL_1161 in contig CP000076_GR.
KEGG	pfl:PFL_1161.
NMPDR	fig|220664.3.peg.2633.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4KHJ1.
OMA	VPVYIGY.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_1161-MON.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PSEF5

Accession

Primary (citable) accession number: Q4KHJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 2, 2005
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)