Reviewed, UniProtKB/Swiss-Prot Q4KHH8 (GLND_PSEF5)

Names and origin

Protein names

Recommended name:
    [Protein-PII] uridylyltransferase
      Short name=PII uridylyl-transferase
    EC=2.7.7.59
Alternative name(s):
    Uridylyl-removing enzyme
    UTase

Gene names

Name:	glnD
Ordered Locus Names:	PFL_1174

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (glnB) regulatory protein By similarity.
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP MF_00277
Sequence similarities	Belongs to the glnD family.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 900	900	[Protein-PII] uridylyltransferase HAMAP MF_00277		PRO_0000192753

Sequences

	Sequence		Length	Mass (Da)
	Q4KHH8-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 40F0D6C35069651E	FASTA	900	103,314
	10 20 30 40 50 60 MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAREVLD TRFRSGREIR RLIEDRAWFI 70 80 90 100 110 120 DNILQKAWDQ FSWSEDADIA LVAVGGYGRG ELHPYSDIDL LILLDSADHE IFRDSIERFL 130 140 150 160 170 180 TLLWDIGLEV GQSVRSVDEC AEQARADLTV ITNLMESRTI AGPEHLRQRM LQVTSTEHMW 190 200 210 220 230 240 PSKDFFLAKR AEQKARHHKY NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLRAL 250 260 270 280 290 300 AGEGFLVESE NALLASSQEF LWKVRYALHM LAGRAEDRLL LDHQRSIATL LGFEGEDAKQ 310 320 330 340 350 360 SIESFMQQYY RVVMSIAQLS DLIIQHFEEV ILAPEDEAPP QPINARFQLH DGYIEAINDN 370 380 390 400 410 420 VFKRTPFAML EIFVLMAQHP EIKGVRADTI RLLREHRHLI DDDFRHDIRN TSLFIELFKC 430 440 450 460 470 480 EIGIHRNLRR MNRYGILGRY LPEFGFIVGQ MQHDLFHIYT VDAHTLNLIK HLRKMQYTPI 490 500 510 520 530 540 SEKFPLASKL MGKLPKPELI YLAGLYHDIG KGRHGDHSEI GAVDAEAFCV RHQLPQWDTR 550 560 570 580 590 600 LIVWLVQNHL VMSTTAQRKD LSDPQVIHDF AQIVVDQTRL DYLYVLTVAD IYATNPTLWN 610 620 630 640 650 660 SWRASLLRQL YTETKRALRR GLENPVDREE QIRQTQSAAL DILVRNGTDP DEVEQLWSQL 670 680 690 700 710 720 GDDYFLRHTA GDVAWHSDAI LQQPADGGPL VLIKEITQRE FEGGTQIFIY APDQHDFFAV 730 740 750 760 770 780 TVAAMDQLNL NIHDARIITS SSQFTLDTYI VLDNDGESIG NNPQRVEQIR KGLTDALRNP 790 800 810 820 830 840 DDYPTIIQRR VPRQLKHFAF APEVTIHNDA QRPVTVLELS APDRPGLLAR IGKIFLEFDL 850 860 870 880 890 900 SLQNAKIATL GERVEDVFFI TDAHNQPLSD PQLCSRLQDA IVEQLSVSHE PTIEMTRLSI

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY90461.1.
RefSeq	YP_258305.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3478991.
GenomeReviews	Gene locus PFL_1174 in contig CP000076_GR.
KEGG	pfl:PFL_1174.
NMPDR	fig|220664.3.peg.2617.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4KHH8.
OMA	Q4KHH8. MQHDLFH.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_1174-MON.
Family and domain databases
HAMAP	MF_00277. [Tree]
InterPro	IPR002912. ACT_bd. IPR010043. GlnD_Uridyltrans. IPR003607. Met-dep_phosphohydro_HD. IPR006674. Met-dep_phosphohydro_HD_sub. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. GlnD_Uridyltrans. 1 hit.
Pfam	PF01842. ACT. 2 hits. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_PSEF5

Accession

Primary (citable) accession number: Q4KHH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)