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Q4KHH8

- GLND_PSEF5

UniProt

Q4KHH8 - GLND_PSEF5

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, PFL_1174
Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPFLU220664:GIX8-1178-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PFL_1174
OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000008540: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000192753Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi220664.PFL_1174.

Structurei

3D structure databases

ProteinModelPortaliQ4KHH8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini481 – 57696HD
Add
BLAST
Domaini706 – 78984ACT 1
Add
BLAST
Domaini816 – 89580ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotation
Add
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KHH8-1 [UniParc]FASTAAdd to Basket

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MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAREVLD TRFRSGREIR    50
RLIEDRAWFI DNILQKAWDQ FSWSEDADIA LVAVGGYGRG ELHPYSDIDL 100
LILLDSADHE IFRDSIERFL TLLWDIGLEV GQSVRSVDEC AEQARADLTV 150
ITNLMESRTI AGPEHLRQRM LQVTSTEHMW PSKDFFLAKR AEQKARHHKY 200
NDTEYNLEPN VKGSPGGLRD IQTILWVARR QYGTLNLRAL AGEGFLVESE 250
NALLASSQEF LWKVRYALHM LAGRAEDRLL LDHQRSIATL LGFEGEDAKQ 300
SIESFMQQYY RVVMSIAQLS DLIIQHFEEV ILAPEDEAPP QPINARFQLH 350
DGYIEAINDN VFKRTPFAML EIFVLMAQHP EIKGVRADTI RLLREHRHLI 400
DDDFRHDIRN TSLFIELFKC EIGIHRNLRR MNRYGILGRY LPEFGFIVGQ 450
MQHDLFHIYT VDAHTLNLIK HLRKMQYTPI SEKFPLASKL MGKLPKPELI 500
YLAGLYHDIG KGRHGDHSEI GAVDAEAFCV RHQLPQWDTR LIVWLVQNHL 550
VMSTTAQRKD LSDPQVIHDF AQIVVDQTRL DYLYVLTVAD IYATNPTLWN 600
SWRASLLRQL YTETKRALRR GLENPVDREE QIRQTQSAAL DILVRNGTDP 650
DEVEQLWSQL GDDYFLRHTA GDVAWHSDAI LQQPADGGPL VLIKEITQRE 700
FEGGTQIFIY APDQHDFFAV TVAAMDQLNL NIHDARIITS SSQFTLDTYI 750
VLDNDGESIG NNPQRVEQIR KGLTDALRNP DDYPTIIQRR VPRQLKHFAF 800
APEVTIHNDA QRPVTVLELS APDRPGLLAR IGKIFLEFDL SLQNAKIATL 850
GERVEDVFFI TDAHNQPLSD PQLCSRLQDA IVEQLSVSHE PTIEMTRLSI 900
Length:900
Mass (Da):103,314
Last modified:August 2, 2005 - v1
Checksum:i40F0D6C35069651E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000076 Genomic DNA. Translation: AAY90461.1.
RefSeqiYP_258305.1. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY90461; AAY90461; PFL_1174.
GeneIDi3478991.
KEGGipfl:PFL_1174.
PATRICi19871555. VBIPseFlu72549_1206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000076 Genomic DNA. Translation: AAY90461.1 .
RefSeqi YP_258305.1. NC_004129.6.

3D structure databases

ProteinModelPortali Q4KHH8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 220664.PFL_1174.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY90461 ; AAY90461 ; PFL_1174 .
GeneIDi 3478991.
KEGGi pfl:PFL_1174.
PATRICi 19871555. VBIPseFlu72549_1206.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PFLU220664:GIX8-1178-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf-5 / ATCC BAA-477.

Entry informationi

Entry nameiGLND_PSEF5
AccessioniPrimary (citable) accession number: Q4KHH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: August 2, 2005
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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