Reviewed, UniProtKB/Swiss-Prot Q4KGH6 (ACEK_PSEF5)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	PFL_1530

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000259153
Regions
	Nucleotide binding	317 – 323	7	ATP By similarity
Sites
	Active site	373	1	By similarity
	Binding site	338	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KGH6-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 4904A32301273C01	FASTA	573	66,095
	10 20 30 40 50 60 MSQSWPAADI ARMILDGFDD YREHFRQITD GARQRFEQAQ WQQAQKASAA RINLYEEKVG 70 80 90 100 110 120 ETVARLHQAF STESLMDVSQ WPLVKSAYIS LIDLRFDDEL SETWYNSIFC GLFSHDLISD 130 140 150 160 170 180 GCMFIHTTRP SLRRARAAQT RSYKPQGELA TMLEQVFADY RFSEDYADLP GDLRRLEAQL 190 200 210 220 230 240 RENLPDWVCK DPELTLELFS SVLYRNKGAY LVGRIFTHEE QWPLVIPLLH REGRGIQIDA 250 260 270 280 290 300 LITDEADVSI IFSFTRSYFM VDVPVPAEFI GFLKRILPGK HIAELYTSIG FYKHGKSEFY 310 320 330 340 350 360 RALINHLAST DDRFIMAPGV RGMVMSVFTL PGFNTVFKII KDRFSPSKNV DRATVIEKYR 370 380 390 400 410 420 LVKSVDRVGR MADTQEFADF RFPLSKFDPA CLEELLEVAP STVAVEGQTV LIRHCWTERR 430 440 450 460 470 480 MTPLNLYLEH ANEAQVREAL EDYGLAIKQL AAANIFPGDM LLKNFGVTRH GRVVFYDYDE 490 500 510 520 530 540 ICFLTEANFR HIPAPRTPED EMASEPWYSI GPHDVFPEEF PPFLFADAGQ RKLFDQLHGE 550 560 570 LYNADYWKGL QEAIRAGKVI DVFPYRRQGL EDE

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY90813.1.
RefSeq	YP_258657.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KGH6.
Genome annotation databases
GeneID	3476696.
GenomeReviews	Gene locus PFL_1530 in contig CP000076_GR.
KEGG	pfl:PFL_1530.
NMPDR	fig|220664.3.peg.3579.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	Q4KGH6.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_1530-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEF5

Accession

Primary (citable) accession number: Q4KGH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 2, 2005
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)