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Protein

Ribosomal RNA large subunit methyltransferase M

Gene

rlmM

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytidine(2498) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(2498) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei183S-adenosyl-L-methionineUniRule annotation1
Binding sitei235S-adenosyl-L-methionineUniRule annotation1
Binding sitei255S-adenosyl-L-methionineUniRule annotation1
Binding sitei271S-adenosyl-L-methionineUniRule annotation1
Active sitei300Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase MUniRule annotation (EC:2.1.1.186UniRule annotation)
Alternative name(s):
23S rRNA (cytidine2498-2'-O)-methyltransferaseUniRule annotation
23S rRNA 2'-O-ribose methyltransferase RlmMUniRule annotation
Gene namesi
Name:rlmMUniRule annotation
Ordered Locus Names:PFL_1930
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000008540 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000704171 – 357Ribosomal RNA large subunit methyltransferase MAdd BLAST357

Proteomic databases

PRIDEiQ4KFD4.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_1930.

Structurei

3D structure databases

ProteinModelPortaliQ4KFD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 219S-adenosyl-L-methionine bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. RlmM subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EZ6. Bacteria.
COG2933. LUCA.
HOGENOMiHOG000247137.
KOiK06968.
OMAiVIFECYQ.
OrthoDBiPOG091H04R8.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01551. 23SrRNA_methyltr_M. 1 hit.
InterProiIPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR011224. rRNA_MeTrfase_M.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF028774. UCP028774. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4KFD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTLFIHCRP GFEGEVCSEI ADHAARLNVA GYAKAKPSTA CAEFICTEDD
60 70 80 90 100
GAQRLMQGLR FPELIFPRQW ARGGFIDLPE TDRISVILAY LANFPQCGSL
110 120 130 140 150
WLEVVDTNDG KELSNFCKKF EAPLRKALTA AGKLVDDATK PRLLLTFKSG
160 170 180 190 200
REVFVGLADA GNSAMWPMGI PRLKFPREAP SRSTLKLEEA WHHFIPRDQW
210 220 230 240 250
DERLHGDMTG VDLGAAPGGW TWQLVNRGML VTAVDNGPMA ESLMDTGLVQ
260 270 280 290 300
HLMADGFTYK PRQPVDWMVC DIVEKPARNA ALLETWIGEG HCREAVVNLK
310 320 330 340 350
LPMRQRYAEV KRLLERIEEG FKARGIRVAI GCKQLYHDRE EVTCHLRRLD

LKKPKSA
Length:357
Mass (Da):40,128
Last modified:August 2, 2005 - v1
Checksum:i5528C2880EA3D0AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY91217.1.
RefSeqiWP_011060250.1. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY91217; AAY91217; PFL_1930.
GeneIDi3477621.
KEGGipfl:PFL_1930.
PATRICi19873103. VBIPseFlu72549_1969.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY91217.1.
RefSeqiWP_011060250.1. NC_004129.6.

3D structure databases

ProteinModelPortaliQ4KFD4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220664.PFL_1930.

Proteomic databases

PRIDEiQ4KFD4.

Protocols and materials databases

DNASUi3477621.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY91217; AAY91217; PFL_1930.
GeneIDi3477621.
KEGGipfl:PFL_1930.
PATRICi19873103. VBIPseFlu72549_1969.

Phylogenomic databases

eggNOGiENOG4105EZ6. Bacteria.
COG2933. LUCA.
HOGENOMiHOG000247137.
KOiK06968.
OMAiVIFECYQ.
OrthoDBiPOG091H04R8.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01551. 23SrRNA_methyltr_M. 1 hit.
InterProiIPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR011224. rRNA_MeTrfase_M.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF028774. UCP028774. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRLMM_PSEF5
AccessioniPrimary (citable) accession number: Q4KFD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.