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Q4KF16

- SYE_PSEF5

UniProt

Q4KF16 - SYE_PSEF5

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Protein
Glutamate--tRNA ligase
Gene
gltX, PFL_2049
Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei254 – 2541ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPFLU220664:GIX8-2059-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:PFL_2049
OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000008540: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Glutamate--tRNA ligaseUniRule annotation
PRO_0000237388Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_2049.

Structurei

3D structure databases

ProteinModelPortaliQ4KF16.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionUniRule annotation
Add
BLAST
Motifi251 – 2555"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiDSHEHHA.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KF16-1 [UniParc]FASTAAdd to Basket

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MTTVRTRIAP SPTGDPHVGT AYIALFNYCF AKQHGGEFIL RIEDTDQLRS    50
TRESEQQIFD ALRWLGINWS EGPDVGGPHG PYRQSERGDI YQKYAQQLVD 100
LGHAFPCFCT AEELDQMRAE QMARGETPRY DGRALLLSKE EVARRLAAGE 150
PHVIRMKVPT EGVCVVPDML RGDVEIPWDR MDMQVLMKTD GLPTYFLANV 200
VDDHLMGITH VLRGEEWLPS APKLILLYEY FGWEQPQLCY MPLLRNPDKS 250
KLSKRKNPTS VTFYERMGFM PEAMLNYLGR MGWSMPDERE KFSLQEMVDN 300
FDLSRVSLGG PIFDIEKLSW LNGQWLRDLP VQEFASRVQT WALNPDYMMK 350
IAPHVQGRVE TFSQIAPLAG FFFAGGVNPD AKLFESKKLS GDQVRQLMQL 400
ILWKLESLRQ WEKDSITATI QAVVESLELK LRDAMPLMFA AITGQASSVS 450
VLDAMEILGP DLTRFRLRQA IDLLGGVSKK ENKEWEKLLG AIA 493
Length:493
Mass (Da):56,129
Last modified:August 2, 2005 - v1
Checksum:i1556602B34CB67A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000076 Genomic DNA. Translation: AAY91334.1.
RefSeqiYP_259167.1. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY91334; AAY91334; PFL_2049.
GeneIDi3479086.
KEGGipfl:PFL_2049.
PATRICi19873341. VBIPseFlu72549_2087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000076 Genomic DNA. Translation: AAY91334.1 .
RefSeqi YP_259167.1. NC_004129.6.

3D structure databases

ProteinModelPortali Q4KF16.
ModBasei Search...

Protein-protein interaction databases

STRINGi 220664.PFL_2049.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY91334 ; AAY91334 ; PFL_2049 .
GeneIDi 3479086.
KEGGi pfl:PFL_2049.
PATRICi 19873341. VBIPseFlu72549_2087.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi DSHEHHA.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci PFLU220664:GIX8-2059-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf-5 / ATCC BAA-477.

Entry informationi

Entry nameiSYE_PSEF5
AccessioniPrimary (citable) accession number: Q4KF16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: August 2, 2005
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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