Q4KEB8 (RTCA_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase
Short name=RNA cyclase
Short name=RNA-3'-phosphate cyclase
EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	PFL_2308

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.
Sequence caution	The sequence AAY91581.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000325188
Regions
	Nucleotide binding	283 – 287	5	ATP By similarity
Sites
	Active site	308	1	Tele-AMP-histidine intermediate By similarity
	Binding site	102	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KEB8 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 52D50817C4331B90	FASTA	342	36,971
	10 20 30 40 50 60 MKQDVVELDG AIGGGQVLRS ALSLSMLTGK TLRIHNIRAR RSRPGLLRQH LTAVLAAAQV 70 80 90 100 110 120 CGARSTGAEL GSQVLSFEPG PIRGGDYRFA IGTAGSCTLV LQTLLPALLR APQPSRVSIS 130 140 150 160 170 180 GGTHNPLAPP VDFLQQAWLP QLRRMGGRVE LQLLRHGFVP AGGGELEAFI QPSELQPLHL 190 200 210 220 230 240 QERGALLGSR AWALSAGLPE HVAERELRRV HDRLQLPREQ LTPVLLDEEY GPGNVLLLEF 250 260 270 280 290 300 AFEHLTELFC GFGQNSLRAE KVADGAIDQA RDWLDSGAAV AEHLADQLLL PMALAGGGSF 310 320 330 340 TTPCMTEHLQ SNIRVIEAFL PVRIEARPLS EQVLQVQCHA LS

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY91581.1. Different initiation.
RefSeq	YP_259415.2. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4KEB8.
SMR	Q4KEB8. Positions 5-332.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KEB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3478377.
GenomeReviews	Gene locus PFL_2308 in contig CP000076_GR.
KEGG	pfl:PFL_2308.
NMPDR	fig|220664.3.peg.3125.
PATRIC	19873885. VBIPseFlu72549_2348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0430.
HOGENOM	HBG683539.
OMA	GGTDVAW.
ProtClustDB	PRK04204.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_2308-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR020719. RNA3'_term_phos_cycl-like_CS. IPR013796. RNA3'_term_phos_cycl_insert. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.30.360.20. G3DSA:3.30.360.20. 1 hit. G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 2 hits.
KO	K01974.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF52913. RNA3'-term_phos_cycl_insert. 1 hit. SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 2 hits.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_PSEF5

Accession

Primary (citable) accession number: Q4KEB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	January 25, 2012
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program