Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Catalase-peroxidase

Gene

katG

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei88Transition state stabilizerUniRule annotation1
Active sitei92Proton acceptorUniRule annotation1
Metal bindingi255Iron (heme axial ligand)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei3661. PfCP01_Pf5.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:PFL_2690
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000008540 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003548631 – 726Catalase-peroxidaseAdd BLAST726

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki91 ↔ 214Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-240)UniRule annotation
Cross-linki214 ↔ 240Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiQ4KD86.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_2690.

Structurei

3D structure databases

ProteinModelPortaliQ4KD86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiTRWSNNF.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4KD86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNESKCPFK HTAGEGTSNR DWWPGQLNLK ILHQHSRLSD PMAEGFDYAA
60 70 80 90 100
EFKTLDLAAV KRDLQALMTD SQPWWPADFG HYGPLFIRMA WHSAGTYRIA
110 120 130 140 150
DGRGGAGGGQ QRFAPLNSWP DNVSLDKARR LIWPIKQKYG RKISWADLII
160 170 180 190 200
LTGNVALESM GFKTFGFAGG RQDVWEPEDN VYWGSETTWL DDQRYSGDRE
210 220 230 240 250
LENPLGAVQM GLIYVNPEGP NGNPDPLAAA RDIRETFARM AMDDEETVAL
260 270 280 290 300
IAGGHTFGKT HGAGPATHVG PEPEAAGLEE QGLGWKSSFG TGVGGDAITS
310 320 330 340 350
GLEVIWTTTP TRWSNDFFDH LFGYEWELTT SPAGAHQWRP KAGAGADSVP
360 370 380 390 400
DPHDPNKRRT PSMLTTDLSL RFDPAYEAIS RRFHEHPEQL AEAFSRAWFK
410 420 430 440 450
LTHRDMGPRA RYLGPEVPAE ELIWQDPIPA VNHPLIDAQD IQQLKGQILN
460 470 480 490 500
SGLSVAQLVS TAWASASTFR GSDKRGGANG ARIRLAPQKD WEVNQPQQLA
510 520 530 540 550
QVLQGLEALQ SAFNSAQSTG KRVSLADLIV LGGCAAVELA AKNAGYSISV
560 570 580 590 600
PFAPGRMDAS QEQTDVESFA VLEPVADGFR NYLKPVSGIT AEALLVDRAQ
610 620 630 640 650
LLTLTAPQLT VLLGGLRVLG ANVGQSPHGV FTSRPGTLSN DFFVNLLDMG
660 670 680 690 700
TQWKPQSEAR DLYEGSDRAT GQYKWSGTRV DLLLGSNSQL RALAEVYAAA
710 720
DAGEQFVKDF VAAWDKVMNL DRFDLR
Length:726
Mass (Da):79,512
Last modified:August 2, 2005 - v1
Checksum:iCF7A18222693C2C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY91963.1.
RefSeqiWP_011060986.1. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY91963; AAY91963; PFL_2690.
GeneIDi3477119.
KEGGipfl:PFL_2690.
PATRICi19874671. VBIPseFlu72549_2741.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY91963.1.
RefSeqiWP_011060986.1. NC_004129.6.

3D structure databases

ProteinModelPortaliQ4KD86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220664.PFL_2690.

Protein family/group databases

PeroxiBasei3661. PfCP01_Pf5.

Proteomic databases

PRIDEiQ4KD86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY91963; AAY91963; PFL_2690.
GeneIDi3477119.
KEGGipfl:PFL_2690.
PATRICi19874671. VBIPseFlu72549_2741.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiTRWSNNF.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_PSEF5
AccessioniPrimary (citable) accession number: Q4KD86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.