ID GLGB_PSEF5 Reviewed; 743 AA. AC Q4KCQ3; DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot. DT 02-AUG-2005, sequence version 1. DT 27-MAR-2024, entry version 115. DE RecName: Full=1,4-alpha-glucan branching enzyme GlgB {ECO:0000255|HAMAP-Rule:MF_00685}; DE EC=2.4.1.18 {ECO:0000255|HAMAP-Rule:MF_00685}; DE AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase {ECO:0000255|HAMAP-Rule:MF_00685}; DE AltName: Full=Alpha-(1->4)-glucan branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685}; DE AltName: Full=Glycogen branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685}; DE Short=BE {ECO:0000255|HAMAP-Rule:MF_00685}; GN Name=glgB {ECO:0000255|HAMAP-Rule:MF_00685}; GN OrderedLocusNames=PFL_2874; OS Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5). OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=220664; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5; RX PubMed=15980861; DOI=10.1038/nbt1110; RA Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., RA Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., RA Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., RA Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C., RA Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, RA Thomashow L.S., Loper J.E.; RT "Complete genome sequence of the plant commensal Pseudomonas fluorescens RT Pf-5."; RL Nat. Biotechnol. 23:873-878(2005). CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from CC growing alpha-1,4-glucan chains and the subsequent attachment of the CC oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP- CC Rule:MF_00685}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18; CC Evidence={ECO:0000255|HAMAP-Rule:MF_00685}; CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis. CC {ECO:0000255|HAMAP-Rule:MF_00685}. CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00685}. CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB CC subfamily. {ECO:0000255|HAMAP-Rule:MF_00685}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000076; AAY92146.1; -; Genomic_DNA. DR RefSeq; WP_011061163.1; NC_004129.6. DR AlphaFoldDB; Q4KCQ3; -. DR SMR; Q4KCQ3; -. DR STRING; 220664.PFL_2874; -. DR CAZy; CBM48; Carbohydrate-Binding Module Family 48. DR CAZy; GH13; Glycoside Hydrolase Family 13. DR KEGG; pfl:PFL_2874; -. DR PATRIC; fig|220664.5.peg.2929; -. DR eggNOG; COG0296; Bacteria. DR HOGENOM; CLU_004245_3_2_6; -. DR UniPathway; UPA00164; -. DR Proteomes; UP000008540; Chromosome. DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-UniRule. DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC. DR GO; GO:0043169; F:cation binding; IEA:InterPro. DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro. DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniRule. DR CDD; cd11322; AmyAc_Glg_BE; 1. DR CDD; cd02855; E_set_GBE_prok_N; 1. DR Gene3D; 3.20.20.80; Glycosidases; 1. DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1. DR Gene3D; 2.60.40.10; Immunoglobulins; 1. DR HAMAP; MF_00685; GlgB; 1. DR InterPro; IPR006048; A-amylase/branching_C. DR InterPro; IPR037439; Branching_enzy. DR InterPro; IPR006407; GlgB. DR InterPro; IPR044143; GlgB_N_E_set_prok. DR InterPro; IPR006047; Glyco_hydro_13_cat_dom. DR InterPro; IPR004193; Glyco_hydro_13_N. DR InterPro; IPR013780; Glyco_hydro_b. DR InterPro; IPR017853; Glycoside_hydrolase_SF. DR InterPro; IPR013783; Ig-like_fold. DR InterPro; IPR014756; Ig_E-set. DR NCBIfam; TIGR01515; branching_enzym; 1. DR PANTHER; PTHR43651; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1. DR PANTHER; PTHR43651:SF3; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1. DR Pfam; PF00128; Alpha-amylase; 1. DR Pfam; PF02806; Alpha-amylase_C; 1. DR Pfam; PF02922; CBM_48; 1. DR PIRSF; PIRSF000463; GlgB; 1. DR SMART; SM00642; Aamy; 1. DR SUPFAM; SSF51445; (Trans)glycosidases; 1. DR SUPFAM; SSF81296; E set domains; 2. DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1. PE 3: Inferred from homology; KW Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism; KW Glycosyltransferase; Transferase. FT CHAIN 1..743 FT /note="1,4-alpha-glucan branching enzyme GlgB" FT /id="PRO_0000260677" FT ACT_SITE 423 FT /note="Nucleophile" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685" FT ACT_SITE 476 FT /note="Proton donor" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00685" SQ SEQUENCE 743 AA; 83646 MW; 836AE2D4125950BE CRC64; MSFSNKEQGK QQPALLPSAK AIDALVRAEH RDPFAILGPH GDGAGGQFIR AFLPGALSVQ VLDRDQQQVL GSLQAGEVPG LFVGHFPEAR PYLLRIQWAG GEQVSEDPYS FGPLLGEMDL YLFAEGNHRD LSSCLGAQLT TVDGIDGVRF AVWAPNARRV SVVGDFNIWD GRRHPMRLRH PSGVWELFIP RLQAGEAYKY EILGPQGILP LKADPMALAT QLPPDTASRV APPLTIDWQD QEWMLARGER QRVDAPLSIY ELHAGSWQCE VDDLGEVARQ YSWAELAERL IPYVKDLGFT HIELMPIMEH PFGGSWGYQP LSQFAPSARY GSPQDFAAFI DACHQAGIGV ILDWVPAHFP TDTHGLAQFD GTALYEYGNP LEGFHQDWDT LIYNLGRTEV HGFMLASALH WLKHFHVDGL RVDAVASMLY RDYSRKAGEW VPNRHGGREN LEAIDFLRHL NDVVALEAPG ALVIAEESTA WPGVSQRTDQ GGLGFAYKWN MGWMHDSLHY IQQDPVYRAH HHNELSFGLV YAWSERFILP ISHDEVVHGK HSLIDKMPGD RWQKFANLRA YLSFMWAHPG KKLLFMGCEF GQWREWNHDQ QLDWYLLQYP EHRGVQQLVG DLNRLYRQYP PLHDQDDVPQ GFQWLIGDDA INSVYAWLRW SKSGEPLLVV ANFTPVPRQG YRVGVPVAGR WSELLNSDSQ LYAGSNYGNG GEAFSEEQSS HGQALSLVLN LPPLAVLILR PDS //