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Q4KCQ3 (GLGB_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:PFL_2874
OrganismPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]
Taxonomic identifier220664 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length743 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7437431,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260677

Sites

Active site4231Nucleophile By similarity
Active site4761Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4KCQ3 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 836AE2D4125950BE

FASTA74383,646
        10         20         30         40         50         60 
MSFSNKEQGK QQPALLPSAK AIDALVRAEH RDPFAILGPH GDGAGGQFIR AFLPGALSVQ 

        70         80         90        100        110        120 
VLDRDQQQVL GSLQAGEVPG LFVGHFPEAR PYLLRIQWAG GEQVSEDPYS FGPLLGEMDL 

       130        140        150        160        170        180 
YLFAEGNHRD LSSCLGAQLT TVDGIDGVRF AVWAPNARRV SVVGDFNIWD GRRHPMRLRH 

       190        200        210        220        230        240 
PSGVWELFIP RLQAGEAYKY EILGPQGILP LKADPMALAT QLPPDTASRV APPLTIDWQD 

       250        260        270        280        290        300 
QEWMLARGER QRVDAPLSIY ELHAGSWQCE VDDLGEVARQ YSWAELAERL IPYVKDLGFT 

       310        320        330        340        350        360 
HIELMPIMEH PFGGSWGYQP LSQFAPSARY GSPQDFAAFI DACHQAGIGV ILDWVPAHFP 

       370        380        390        400        410        420 
TDTHGLAQFD GTALYEYGNP LEGFHQDWDT LIYNLGRTEV HGFMLASALH WLKHFHVDGL 

       430        440        450        460        470        480 
RVDAVASMLY RDYSRKAGEW VPNRHGGREN LEAIDFLRHL NDVVALEAPG ALVIAEESTA 

       490        500        510        520        530        540 
WPGVSQRTDQ GGLGFAYKWN MGWMHDSLHY IQQDPVYRAH HHNELSFGLV YAWSERFILP 

       550        560        570        580        590        600 
ISHDEVVHGK HSLIDKMPGD RWQKFANLRA YLSFMWAHPG KKLLFMGCEF GQWREWNHDQ 

       610        620        630        640        650        660 
QLDWYLLQYP EHRGVQQLVG DLNRLYRQYP PLHDQDDVPQ GFQWLIGDDA INSVYAWLRW 

       670        680        690        700        710        720 
SKSGEPLLVV ANFTPVPRQG YRVGVPVAGR WSELLNSDSQ LYAGSNYGNG GEAFSEEQSS 

       730        740 
HGQALSLVLN LPPLAVLILR PDS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000076 Genomic DNA. Translation: AAY92146.1.
RefSeqYP_259980.1. NC_004129.6.

3D structure databases

ProteinModelPortalQ4KCQ3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING220664.PFL_2874.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY92146; AAY92146; PFL_2874.
GeneID3477945.
KEGGpfl:PFL_2874.
PATRIC19875049. VBIPseFlu72549_2929.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycPFLU220664:GIX8-2888-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_PSEF5
AccessionPrimary (citable) accession number: Q4KCQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: August 2, 2005
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries