Q4KBW8 (GLSA_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase
EC=3.5.1.2

Gene names

Name:	glsA
Ordered Locus Names:	PFL_3159

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group › Pseudomonas protegens

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from sequence or structural similarity. Source: JCVI
Molecular_function	glutaminase activity Inferred from sequence or structural similarity. Source: JCVI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Glutaminase HAMAP-Rule MF_00313		PRO_0000336036
Sites
	Binding site	61	1	Substrate By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	238	1	Substrate By similarity
	Binding site	256	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4KBW8 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: D339093D3D1E840E	FASTA	302	32,763
	10 20 30 40 50 60 MQDLLNQILD EVRPLIGQGQ VANYIPALAD VPANQLGIAV YGNDGQVFSA GDADTPFSVQ 70 80 90 100 110 120 SISKVFSLVQ AIGHSGEAIW ERLGHEPSGQ PFNSLVQLEF ERGRPRNPFI NAGALVICDI 130 140 150 160 170 180 NQSRFAAPAL SMRDFVRRLS GNLQVMVDGR VAESEYQHRA RNAAMAYLMQ SFGNFHNDVE 190 200 210 220 230 240 AVLRSYFSHC ALRMSCVDLA RAFCFLANDG FCKHSGEQIL TRRQTQQVNS IMATSGLYDE 250 260 270 280 290 300 AGNFAYRVGL PGKSGVGGGI VAVVPGQFTV CVWSPELNSA GNSLVGMAAL ELLSQRIGWS VF

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY92429.2.
RefSeq	YP_260265.2. NC_004129.6.
3D structure databases
HSSP	HSSP built from PDB template 1MKI based on UniProtKB O31465.
ProteinModelPortal	Q4KBW8.
ModBase	Search...
Protein-protein interaction databases
STRING	220664.PFL_3159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY92429; AAY92429; PFL_3159.
GeneID	3475993.
KEGG	pfl:PFL_3159.
PATRIC	19875635. VBIPseFlu72549_3222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
ProtClustDB	PRK00971.
Enzyme and pathway databases
BioCyc	PFLU220664:GIX8-3174-MONOMER.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA_PSEF5

Accession

Primary (citable) accession number: Q4KBW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program