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Protein

Glutaminase

Gene

glsA

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61SubstrateUniRule annotation1
Binding sitei111SubstrateUniRule annotation1
Binding sitei155SubstrateUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Binding sitei186SubstrateUniRule annotation1
Binding sitei238SubstrateUniRule annotation1
Binding sitei256Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciPPRO220664:G1G4K-3241-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
GlutaminaseUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:glsAUniRule annotation
Ordered Locus Names:PFL_3159
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000008540 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003360361 – 302GlutaminaseAdd BLAST302

Proteomic databases

PRIDEiQ4KBW8

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_3159

Structurei

3D structure databases

ProteinModelPortaliQ4KBW8
SMRiQ4KBW8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutaminase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSV Bacteria
COG2066 LUCA
HOGENOMiHOG000216890
KOiK01425
OMAiEPSGNPF
OrthoDBiPOG091H02PU

Family and domain databases

HAMAPiMF_00313 Glutaminase, 1 hit
InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR015868 Glutaminase
PANTHERiPTHR12544 PTHR12544, 1 hit
PfamiView protein in Pfam
PF04960 Glutaminase, 1 hit
SUPFAMiSSF56601 SSF56601, 1 hit
TIGRFAMsiTIGR03814 Gln_ase, 1 hit

Sequencei

Sequence statusi: Complete.

Q4KBW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDLLNQILD EVRPLIGQGQ VANYIPALAD VPANQLGIAV YGNDGQVFSA
60 70 80 90 100
GDADTPFSVQ SISKVFSLVQ AIGHSGEAIW ERLGHEPSGQ PFNSLVQLEF
110 120 130 140 150
ERGRPRNPFI NAGALVICDI NQSRFAAPAL SMRDFVRRLS GNLQVMVDGR
160 170 180 190 200
VAESEYQHRA RNAAMAYLMQ SFGNFHNDVE AVLRSYFSHC ALRMSCVDLA
210 220 230 240 250
RAFCFLANDG FCKHSGEQIL TRRQTQQVNS IMATSGLYDE AGNFAYRVGL
260 270 280 290 300
PGKSGVGGGI VAVVPGQFTV CVWSPELNSA GNSLVGMAAL ELLSQRIGWS

VF
Length:302
Mass (Da):32,763
Last modified:May 20, 2008 - v2
Checksum:iD339093D3D1E840E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA Translation: AAY92429.2
RefSeqiWP_011061446.1, NC_004129.6

Genome annotation databases

EnsemblBacteriaiAAY92429; AAY92429; PFL_3159
GeneIDi29827123
KEGGipfl:PFL_3159
PATRICifig|220664.5.peg.3222

Similar proteinsi

Entry informationi

Entry nameiGLSA_PSEF5
AccessioniPrimary (citable) accession number: Q4KBW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 23, 2018
This is version 66 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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