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Q4KBW8

- GLSA_PSEF5

UniProt

Q4KBW8 - GLSA_PSEF5

Protein

Glutaminase

Gene

glsA

Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 2 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611SubstrateUniRule annotation
    Binding sitei111 – 1111SubstrateUniRule annotation
    Binding sitei155 – 1551SubstrateUniRule annotation
    Binding sitei162 – 1621SubstrateUniRule annotation
    Binding sitei186 – 1861SubstrateUniRule annotation
    Binding sitei238 – 2381SubstrateUniRule annotation
    Binding sitei256 – 2561Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. glutaminase activity Source: JCVI

    GO - Biological processi

    1. glutamine metabolic process Source: JCVI

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciPFLU220664:GIX8-3174-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GlutaminaseUniRule annotation (EC:3.5.1.2UniRule annotation)
    Gene namesi
    Name:glsAUniRule annotation
    Ordered Locus Names:PFL_3159
    OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
    Taxonomic identifieri220664 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000008540: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 302302GlutaminasePRO_0000336036Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi220664.PFL_3159.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4KBW8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutaminase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2066.
    HOGENOMiHOG000216890.
    KOiK01425.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF04960. Glutaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4KBW8-1 [UniParc]FASTAAdd to Basket

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    MQDLLNQILD EVRPLIGQGQ VANYIPALAD VPANQLGIAV YGNDGQVFSA    50
    GDADTPFSVQ SISKVFSLVQ AIGHSGEAIW ERLGHEPSGQ PFNSLVQLEF 100
    ERGRPRNPFI NAGALVICDI NQSRFAAPAL SMRDFVRRLS GNLQVMVDGR 150
    VAESEYQHRA RNAAMAYLMQ SFGNFHNDVE AVLRSYFSHC ALRMSCVDLA 200
    RAFCFLANDG FCKHSGEQIL TRRQTQQVNS IMATSGLYDE AGNFAYRVGL 250
    PGKSGVGGGI VAVVPGQFTV CVWSPELNSA GNSLVGMAAL ELLSQRIGWS 300
    VF 302
    Length:302
    Mass (Da):32,763
    Last modified:May 20, 2008 - v2
    Checksum:iD339093D3D1E840E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY92429.2.
    RefSeqiYP_260265.2. NC_004129.6.

    Genome annotation databases

    EnsemblBacteriaiAAY92429; AAY92429; PFL_3159.
    GeneIDi3475993.
    KEGGipfl:PFL_3159.
    PATRICi19875635. VBIPseFlu72549_3222.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000076 Genomic DNA. Translation: AAY92429.2 .
    RefSeqi YP_260265.2. NC_004129.6.

    3D structure databases

    ProteinModelPortali Q4KBW8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 220664.PFL_3159.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY92429 ; AAY92429 ; PFL_3159 .
    GeneIDi 3475993.
    KEGGi pfl:PFL_3159.
    PATRICi 19875635. VBIPseFlu72549_3222.

    Phylogenomic databases

    eggNOGi COG2066.
    HOGENOMi HOG000216890.
    KOi K01425.

    Enzyme and pathway databases

    BioCyci PFLU220664:GIX8-3174-MONOMER.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    HAMAPi MF_00313. Glutaminase.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view ]
    PANTHERi PTHR12544. PTHR12544. 1 hit.
    Pfami PF04960. Glutaminase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56601. SSF56601. 1 hit.
    TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf-5 / ATCC BAA-477.

    Entry informationi

    Entry nameiGLSA_PSEF5
    AccessioniPrimary (citable) accession number: Q4KBW8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 52 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3