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Q4KBW8

- GLSA_PSEF5

UniProt

Q4KBW8 - GLSA_PSEF5

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Protein

Glutaminase

Gene
glsA, PFL_3159
Organism
Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611Substrate By similarity
Binding sitei111 – 1111Substrate By similarity
Binding sitei155 – 1551Substrate By similarity
Binding sitei162 – 1621Substrate By similarity
Binding sitei186 – 1861Substrate By similarity
Binding sitei238 – 2381Substrate By similarity
Binding sitei256 – 2561Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. glutaminase activity Source: JCVI

GO - Biological processi

  1. glutamine metabolic process Source: JCVI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciPFLU220664:GIX8-3174-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase (EC:3.5.1.2)
Gene namesi
Name:glsA
Ordered Locus Names:PFL_3159
OrganismiPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000008540: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302GlutaminaseUniRule annotationPRO_0000336036Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi220664.PFL_3159.

Structurei

3D structure databases

ProteinModelPortaliQ4KBW8.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutaminase family.

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
KOiK01425.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF04960. Glutaminase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4KBW8-1 [UniParc]FASTAAdd to Basket

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MQDLLNQILD EVRPLIGQGQ VANYIPALAD VPANQLGIAV YGNDGQVFSA    50
GDADTPFSVQ SISKVFSLVQ AIGHSGEAIW ERLGHEPSGQ PFNSLVQLEF 100
ERGRPRNPFI NAGALVICDI NQSRFAAPAL SMRDFVRRLS GNLQVMVDGR 150
VAESEYQHRA RNAAMAYLMQ SFGNFHNDVE AVLRSYFSHC ALRMSCVDLA 200
RAFCFLANDG FCKHSGEQIL TRRQTQQVNS IMATSGLYDE AGNFAYRVGL 250
PGKSGVGGGI VAVVPGQFTV CVWSPELNSA GNSLVGMAAL ELLSQRIGWS 300
VF 302
Length:302
Mass (Da):32,763
Last modified:May 20, 2008 - v2
Checksum:iD339093D3D1E840E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000076 Genomic DNA. Translation: AAY92429.2.
RefSeqiYP_260265.2. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY92429; AAY92429; PFL_3159.
GeneIDi3475993.
KEGGipfl:PFL_3159.
PATRICi19875635. VBIPseFlu72549_3222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000076 Genomic DNA. Translation: AAY92429.2 .
RefSeqi YP_260265.2. NC_004129.6.

3D structure databases

ProteinModelPortali Q4KBW8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 220664.PFL_3159.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY92429 ; AAY92429 ; PFL_3159 .
GeneIDi 3475993.
KEGGi pfl:PFL_3159.
PATRICi 19875635. VBIPseFlu72549_3222.

Phylogenomic databases

eggNOGi COG2066.
HOGENOMi HOG000216890.
KOi K01425.

Enzyme and pathway databases

BioCyci PFLU220664:GIX8-3174-MONOMER.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
HAMAPi MF_00313. Glutaminase.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view ]
PANTHERi PTHR12544. PTHR12544. 1 hit.
Pfami PF04960. Glutaminase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf-5 / ATCC BAA-477.

Entry informationi

Entry nameiGLSA_PSEF5
AccessioniPrimary (citable) accession number: Q4KBW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 11, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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