Reviewed, UniProtKB/Swiss-Prot Q4KBL2 (NUDC_PSEF5)

Names and origin

Protein names

Recommended name:
    NADH pyrophosphatase
    EC=3.6.1.22

Gene names

Name:	nudC
Ordered Locus Names:	PFL_3266

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	NAD+ + H2O = AMP + NMN. HAMAP MF_00297
Cofactor	Divalent ions. Manganese or magnesium By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. NudC subfamily.

Ontologies

Keywords
Ligand	Magnesium Manganese NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	NAD+ diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	NADH pyrophosphatase HAMAP MF_00297		PRO_0000232117
Regions
	Motif	173 – 194	22	Nudix box HAMAP MF_00297

Sequences

	Sequence		Length	Mass (Da)
	Q4KBL2-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 923F7D9BA54595DB	FASTA	276	30,708
	10 20 30 40 50 60 MIERWTTAVL DTDLPGGWAV ARGPDGFLYD DNGALFPRHW LKGQDLPLLA EHGIGHLDGE 70 80 90 100 110 120 PVYLLELSAR AEVPGCGWKG LRAFMLEGDH TLYKVLGYAA QIGTWAREHR YCGSCGRPMA 130 140 150 160 170 180 QVPGERAMYC QPCDLRSYPR ISPSMIVLIT RGDEILLARS PRFVTGVYST LAGFAEPGES 190 200 210 220 230 240 AEDCLIREVR EEVSIEVRNI QYVGSQCWPF PHSMMLGFHA EYAGGEIIPQ EDEIEDAQWF 250 260 270 SVHALPPLPA SRSIARYLID LYVARRLGHA EPVLPG

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY92535.1.
RefSeq	YP_260371.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3477808.
GenomeReviews	Gene locus PFL_3266 in contig CP000076_GR.
KEGG	pfl:PFL_3266.
NMPDR	fig|220664.3.peg.4539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4KBL2.
OMA	Q4KBL2. TWAREHR.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_3266-MON.
Family and domain databases
HAMAP	MF_00297. [Tree]
InterPro	IPR015375. NADH_PPase-like_N. IPR000086. NUDIX_hydrolase_core. IPR015376. Znr_NADH_PPase. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. PF09296. NUDIX-like. 1 hit. PF09297. zf-NADH-PPase. 1 hit. [Graphical view]
PROSITE	PS00893. NUDIX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUDC_PSEF5

Accession

Primary (citable) accession number: Q4KBL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)