Reviewed, UniProtKB/Swiss-Prot Q4KAD4 (DNAE2_PSEF5)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	PFL_3697

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	1034 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1034	1034	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103389

Sequences

	Sequence		Length	Mass (Da)
	Q4KAD4-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 21B064CB3642664D	FASTA	1,034	115,233
	10 20 30 40 50 60 MSAYAELHCL SNFSFQRGAS SAAELFQRAK ALGYQALAIT DECTLAGIVR AWQASRDTGL 70 80 90 100 110 120 KLIVGSEMQV EDGPRLVLLV EDLAGYQGLC RLITRARRRA EKGSYRLLRE DFAEPLPGLL 130 140 150 160 170 180 ALWLTGAGEP LAQGRWLQQV FPQRLWLALE LHCGQDDARH LQQQLALARQ LGLPAVACGD 190 200 210 220 230 240 VHMHVRGRRA LQDTMTAIRH HLPVAEAGQR LFANGERHLR PVEVLQGLYP QALLDESLAI 250 260 270 280 290 300 AGRCAFDLGQ LRYQYPRELV PEGHDAGSWL RVLTQRGIVR RWPTGARAEV LKQIDKELTL 310 320 330 340 350 360 ICDLGYESYF LTVHDIVDFA RRQHILCQGR GSAANSVVCF ALGITEIDPD RSTLLFERFL 370 380 390 400 410 420 SRERNEPPDI DVDFEHERRE EVLQYLFQRY GRHRAALTAV VSTYHGAGAV RDVAKALGLP 430 440 450 460 470 480 PDQVNALADC CGHWSDTPPS VERLQEAGFD PQSPVLRRVL SLTGQLIGFP RHLSQHPGGF 490 500 510 520 530 540 VISEQPLDHL VPVENATMAE RTVIQWDKDD LDMVGLLKVD ILALGMLSAI RRCFDLIHGY 550 560 570 580 590 600 RGQRYSLASI PPEDPATYEM IGRADTIGVF QIESRAQMSM LPRLKPQNFY DLVIEVAIVR 610 620 630 640 650 660 PGPIQGGMVH PYLRRRNRQE PETYPSPELE GVLKRTLGVP LFQEQVMQIA IVAADYTPGE 670 680 690 700 710 720 ADQLRRSMAA WKRHGGLEPH RERLRRGMNR NGYSDEFATQ IFEQIKGFGS YGFPESHAAS 730 740 750 760 770 780 FALLTYASCW LKCHEPAAFA CALINSWPMG FYSPDQILQD ARRHGLQVLA VDVGASDWDC 790 800 810 820 830 840 SLEPIPVQPA RAGNGPPGQP ALRLGLRMIK GFREDDARRI ERARRQRAFV DIADLGERAR 850 860 870 880 890 900 LDVRAQELLA DAGALQALAG DRYRARWEVA GVERQLGLFA GLPSQEEAPV ALPRPSVGED 910 920 930 940 950 960 LQADYHSLGT TLGPHPLALL RPQLAARRCR SSRDLLAVEH GRSVSVAGLV TGRQRPGTAS 970 980 990 1000 1010 1020 GVTFVTLEDE FGNVNVVVWR DLAERQRRVL VGAQLLKVDG TLETEGEVRH LIAGRLSDLS 1030 PLLDGIHVRS RDFR

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY92963.1.
RefSeq	YP_260799.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4KAD4.
Genome annotation databases
GeneID	3476970.
GenomeReviews	Gene locus PFL_3697 in contig CP000076_GR.
KEGG	pfl:PFL_3697.
NMPDR	fig|220664.3.peg.5519.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	MGFYSPD.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_3697-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSEF5

Accession

Primary (citable) accession number: Q4KAD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)