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Q4K909 (DSBD_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thiol:disulfide interchange protein DsbD
EC=1.8.1.8
Alternative name(s):
Protein-disulfide reductase
Short name=Disulfide reductase
Gene names
Name:dsbD
Ordered Locus Names:PFL_4182
OrganismPseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]
Taxonomic identifier220664 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity. HAMAP MF_00399

Catalytic activity

Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00399.

Sequence similarities

Belongs to the thioredoxin family. DsbD subfamily.

Contains 1 thioredoxin domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 583565Thiol:disulfide interchange protein DsbD HAMAP MF_00399
PRO_0000304393

Regions

Transmembrane168 – 18821Helical; Potential
Transmembrane214 – 23421Helical; Potential
Transmembrane245 – 26521Helical; Potential
Transmembrane289 – 30921Helical; Potential
Transmembrane326 – 34621Helical; Potential
Transmembrane359 – 37921Helical; Potential
Transmembrane382 – 40221Helical; Potential
Transmembrane413 – 43321Helical; Potential
Domain458 – 581124Thioredoxin

Amino acid modifications

Disulfide bond118 ↔ 124Redox-active By similarity
Disulfide bond186 ↔ 306Redox-active By similarity
Disulfide bond496 ↔ 499Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4K909 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: CAEBB141CBA77113

FASTA58362,515
        10         20         30         40         50         60 
MRRLFLLLFM LFTTLAHAGN NPFEVKPDFL PVGKAFVFTS ERLPSGETQL FWQIADGYYL 

        70         80         90        100        110        120 
YQKRLKFDGV APELQPTLPA GEDHSDEFFG QQTVYRQGLE VKLPAAASGK VKLGWQGCAD 

       130        140        150        160        170        180 
AGLCYPPQTL EVDLGGAPAV AASNVATTSN GSAQDQLLAN DLQHKSWGLG LLAFFGFGLL 

       190        200        210        220        230        240 
LAFAPCSLPM LPILAGMVVG SGASPRRGLA LASSYVLCMA LVYAGMGVIA ALLGSNLQAW 

       250        260        270        280        290        300 
LQQPWVLGSF AALFVLLSLP MFGFFELQMP ALLRDRLEGA SRQRQGGSLI GCGILGALSA 

       310        320        330        340        350        360 
LLVGPCMTAP LAGGLLYIAQ TGNALFGGLA LFALGLGIGL PLLLLVTLGN RFLPKPGPWM 

       370        380        390        400        410        420 
NLLKGVFGIL FLGTAIYMLR PVLNPGLWMG LWGALALVVA YCAWQQRAIA GRLLHLFGAG 

       430        440        450        460        470        480 
ALLFAAWGGM LLVGAAGGGD DLWRPLKVYR GGPVANSVTA HDAFTTVKSP AELQGALDSA 

       490        500        510        520        530        540 
RAQGQWVLLD YYADWCVSCK IMEKTVFGQP QVLEALKDVR LLRLDVTLDN ADGRELLSRY 

       550        560        570        580 
KVPGPPSMLW IGPDGSERRS QRITGEVDAN AFLQRWTQTR EAR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000076 Genomic DNA. Translation: AAY93438.1.
RefSeqYP_261274.1. NC_004129.6.

3D structure databases

HSSPHSSP built from PDB template 1JPE based on UniProtKB P36655.
ProteinModelPortalQ4K909.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ4K909.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3476920.
GenomeReviewsGene locus PFL_4182 in contig CP000076_GR.
KEGGpfl:PFL_4182.
NMPDRfig|220664.3.peg.5994.
PATRIC19877771. VBIPseFlu72549_4279.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4232.
HOGENOMHBG640883.
OMAFLAIWIA.
PhylomeDBQ4K909.
ProtClustDBCLSK867296.

Enzyme and pathway databases

BioCycPFLU220664:PFL_4182-MONOMER.

Family and domain databases

HAMAPMF_00399. DbsD.
[Tree]
InterProIPR003834. Cyt_c_assmbl_TM_dom.
IPR022910. Thiol_diS_interchange_DbsD.
IPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KOK04084.
PANTHERPTHR10438. Trx. 1 hit.
PfamPF02683. DsbD. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMSSF52833. Thiordxn-like_fd. 1 hit.
PROSITEPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDSBD_PSEF5
AccessionPrimary (citable) accession number: Q4K909
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 2, 2005
Last modified: January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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