Reviewed, UniProtKB/Swiss-Prot Q4K8U2 (TPMT_PSEF5)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	PFL_4249

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_0000220127
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K8U2-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 6F44DDDC028B27EE	FASTA	222	25,217
	10 20 30 40 50 60 MQADFWQKRW ERDQIGFHLA EVNPYLQQYW PALGLAAQSR VLVPLCGKSL DLIWLADQGF 70 80 90 100 110 120 EVLGIELAEK AVEDFFREHQ LQPQISQHGV FKVYAHGPIE LWCGDFFALT AEDTADCTAL 130 140 150 160 170 180 YDRAAMIALP SPMRQRYAAH LNGLLPNGSK GLLITMDYAQ EQLDGPPFAV LDDEVRQRLG 190 200 210 220 GVWQLQVETE RDILGESWKF LQGGVTRLVE RVYRLSKQGA AR

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY93505.1.
RefSeq	YP_261341.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3477375.
GenomeReviews	Gene locus PFL_4249 in contig CP000076_GR.
KEGG	pfl:PFL_4249.
NMPDR	fig|220664.3.peg.6061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4K8U2.
OMA	Q4K8U2. PPFAVSP.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_4249-MON.
Family and domain databases
HAMAP	MF_00812. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_PSEF5

Accession

Primary (citable) accession number: Q4K8U2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)