Q4K8M4 (UBIG_PSEF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-demethylubiquinone-9 3-methyltransferase
EC=2.1.1.64

Alternative name(s):
2-polyprenyl-6-hydroxyphenyl methylase
EC=2.1.1.222
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase
Short name=DHHB methyltransferase

Gene names

Name:	ubiG
Ordered Locus Names:	PFL_4317

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-specific O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway By similarity.
Catalytic activity	S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n. HAMAP MF_00472 S-adenosyl-L-methionine + 3-(all-trans-polyprenyl)benzene-1,2-diol = S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-polyprenyl)phenol.
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP MF_00472
Sequence similarities	Belongs to the methyltransferase superfamily. UbiG/COQ3 family.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Inferred from electronic annotation. Source: InterPro 3-demethylubiquinone-9 3-O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	3-demethylubiquinone-9 3-methyltransferase HAMAP MF_00472		PRO_0000241718

Sequences

	Sequence		Length	Mass (Da)
	Q4K8M4 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: F6FC57B3A208F591	FASTA	232	25,966
	10 20 30 40 50 60 MSNVDHAEIA KFEALAHRWW DRESEFKPLH DINPLRVNWI DERVGLAGKK VLDVGCGGGI 70 80 90 100 110 120 LSEAMAQRGA TVTGIDMGEA PLAVAQLHQL ESGVNVEYRQ ITAEALAEEM PEQFDVVTCL 130 140 150 160 170 180 EMLEHVPDPS SVIRACFRMV KPGGQVFFST INRNPKAYLF AIIGAEYIMK LLPRGTHDFK 190 200 210 220 230 KFIRPSELGA WSRSAGLTVK DIIGLTYNPL TKHYKLAPDV DVNYMIQTLR EE

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf-5 / ATCC BAA-477.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY93572.1.
RefSeq	YP_261409.1. NC_004129.6.
3D structure databases
ProteinModelPortal	Q4K8M4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K8M4.
2D gel databases
World-2DPAGE	0008:Q4K8M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3478440.
GenomeReviews	Gene locus PFL_4317 in contig CP000076_GR.
KEGG	pfl:PFL_4317.
NMPDR	fig|220664.3.peg.6129.
PATRIC	19878058. VBIPseFlu72549_4422.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2227.
HOGENOM	HBG738842.
OMA	DHAEIAK.
PhylomeDB	Q4K8M4.
ProtClustDB	PRK05134.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_4317-MONOMER.
Family and domain databases
HAMAP	MF_00472. UbiG. [Tree]
InterPro	IPR013216. Methyltransf_11. IPR010233. UbiG_MeTrfase. [Graphical view]
KO	K00568.
PANTHER	PTHR23134. PTHR23134. 1 hit.
Pfam	PF08241. Methyltransf_11. 1 hit. [Graphical view]
TIGRFAMs	TIGR01983. UbiG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UBIG_PSEF5

Accession

Primary (citable) accession number: Q4K8M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program