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Protein

Adenosylcobinamide-GDP ribazoletransferase

Gene

cobS

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.UniRule annotation

Catalytic activityi

Adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin.UniRule annotation
Adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate = GMP + adenosylcobalamin 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00148; UER00238.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylcobinamide-GDP ribazoletransferaseUniRule annotation (EC:2.7.8.26UniRule annotation)
Alternative name(s):
Cobalamin synthaseUniRule annotation
Cobalamin-5'-phosphate synthaseUniRule annotation
Gene namesi
Name:cobSUniRule annotation
Ordered Locus Names:PFL_4422
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000008540 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei31 – 48HelicalUniRule annotationAdd BLAST18
Transmembranei61 – 81HelicalUniRule annotationAdd BLAST21
Transmembranei109 – 129HelicalUniRule annotationAdd BLAST21
Transmembranei134 – 154HelicalUniRule annotationAdd BLAST21
Transmembranei188 – 208HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000457911 – 243Adenosylcobinamide-GDP ribazoletransferaseAdd BLAST243

Interactioni

Protein-protein interaction databases

STRINGi220664.PFL_4422.

Family & Domainsi

Sequence similaritiesi

Belongs to the CobS family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GN9. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
KOiK02233.
OMAiGAWNWLV.
OrthoDBiPOG091H02RG.

Family and domain databases

HAMAPiMF_00719. CobS. 1 hit.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4K8C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPFWIALQF LSSLPVRLPG MPRPEELGRS LLFYPLVGLL FGVLLWVLNA
60 70 80 90 100
MLGGVPLLLH AALLLTAWVL LSGGLHLDGL ADSADAWLGG FGDRERTLSI
110 120 130 140 150
MKDPRSGPIA VVTLVLVLLL KFTALVALIE QQQGFALLLA PLIGRGALLG
160 170 180 190 200
LFLCTPYVRA GGLGQALADH LPRVAGRQVL GLSVLACLLL GGYSGLWAVL
210 220 230 240
LATVLFFWLR QVMMRRLGGT TGDTAGAVLE LLETAVLLGV ALF
Length:243
Mass (Da):25,865
Last modified:August 2, 2005 - v1
Checksum:iD718B321E700E2BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY93673.1.
RefSeqiWP_011062686.1. NC_004129.6.

Genome annotation databases

EnsemblBacteriaiAAY93673; AAY93673; PFL_4422.
GeneIDi3478541.
KEGGipfl:PFL_4422.
PATRICi19878276. VBIPseFlu72549_4527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA. Translation: AAY93673.1.
RefSeqiWP_011062686.1. NC_004129.6.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220664.PFL_4422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY93673; AAY93673; PFL_4422.
GeneIDi3478541.
KEGGipfl:PFL_4422.
PATRICi19878276. VBIPseFlu72549_4527.

Phylogenomic databases

eggNOGiENOG4105GN9. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
KOiK02233.
OMAiGAWNWLV.
OrthoDBiPOG091H02RG.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00238.

Family and domain databases

HAMAPiMF_00719. CobS. 1 hit.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOBS_PSEF5
AccessioniPrimary (citable) accession number: Q4K8C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.