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Protein

Adenosylcobinamide-GDP ribazoletransferase

Gene

cobS

Organism
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.UniRule annotation

Catalytic activityi

Adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin.UniRule annotation
Adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate = GMP + adenosylcobalamin 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Cob(I)yrinic acid a,c-diamide adenosyltransferase (cobO)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Bifunctional adenosylcobalamin biosynthesis protein (cobU)
  6. Bifunctional adenosylcobalamin biosynthesis protein (cobU)
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCobalamin biosynthesis
LigandMagnesium

Enzyme and pathway databases

BioCyciPPRO220664:G1G4K-4550-MONOMER
UniPathwayiUPA00148; UER00238

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylcobinamide-GDP ribazoletransferaseUniRule annotation (EC:2.7.8.26UniRule annotation)
Alternative name(s):
Cobalamin synthaseUniRule annotation
Cobalamin-5'-phosphate synthaseUniRule annotation
Gene namesi
Name:cobSUniRule annotation
Ordered Locus Names:PFL_4422
OrganismiPseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
Taxonomic identifieri220664 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei31 – 48HelicalUniRule annotationAdd BLAST18
Transmembranei61 – 81HelicalUniRule annotationAdd BLAST21
Transmembranei109 – 129HelicalUniRule annotationAdd BLAST21
Transmembranei134 – 154HelicalUniRule annotationAdd BLAST21
Transmembranei188 – 208HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000457911 – 243Adenosylcobinamide-GDP ribazoletransferaseAdd BLAST243

Proteomic databases

PRIDEiQ4K8C3

Interactioni

Protein-protein interaction databases

STRINGi220664.PFL_4422

Family & Domainsi

Sequence similaritiesi

Belongs to the CobS family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GN9 Bacteria
COG0368 LUCA
HOGENOMiHOG000228087
KOiK02233
OMAiGHTGDTY
OrthoDBiPOG091H02RG

Family and domain databases

HAMAPiMF_00719 CobS, 1 hit
InterProiView protein in InterPro
IPR003805 CobS
PANTHERiPTHR34148 PTHR34148, 1 hit
PfamiView protein in Pfam
PF02654 CobS, 1 hit
TIGRFAMsiTIGR00317 cobS, 1 hit

Sequencei

Sequence statusi: Complete.

Q4K8C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPFWIALQF LSSLPVRLPG MPRPEELGRS LLFYPLVGLL FGVLLWVLNA
60 70 80 90 100
MLGGVPLLLH AALLLTAWVL LSGGLHLDGL ADSADAWLGG FGDRERTLSI
110 120 130 140 150
MKDPRSGPIA VVTLVLVLLL KFTALVALIE QQQGFALLLA PLIGRGALLG
160 170 180 190 200
LFLCTPYVRA GGLGQALADH LPRVAGRQVL GLSVLACLLL GGYSGLWAVL
210 220 230 240
LATVLFFWLR QVMMRRLGGT TGDTAGAVLE LLETAVLLGV ALF
Length:243
Mass (Da):25,865
Last modified:August 2, 2005 - v1
Checksum:iD718B321E700E2BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000076 Genomic DNA Translation: AAY93673.1
RefSeqiWP_011062686.1, NC_004129.6

Genome annotation databases

EnsemblBacteriaiAAY93673; AAY93673; PFL_4422
KEGGipfl:PFL_4422
PATRICifig|220664.5.peg.4527

Entry informationi

Entry nameiCOBS_PSEF5
AccessioniPrimary (citable) accession number: Q4K8C3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 2, 2005
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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