Reviewed, UniProtKB/Swiss-Prot Q4K837 (ASTD_PSEF5)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	PFL_4512

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262414
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K837-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 5C4F96D672036DEE	FASTA	488	51,299
	10 20 30 40 50 60 MSTLYIAGQW LAGQGEAFTS VNPVTQAVIW SGNGATAAQV ESAVQAARQA FPAWAKRSLE 70 80 90 100 110 120 ERISVLEAFA ASLKSRADEL ARCIGEETGK PLWEAATEVT SMVNKIAISV QSYRERTGEK 130 140 150 160 170 180 SGPLGDATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNSVLFKP SELTPKVAEL 190 200 210 220 230 240 TVQCWIEAGL PAGVLNLLQG ARETGIALAA NPGIDGLFFT GSSRTGNHLH QQFAGRPDKI 250 260 270 280 290 300 LALEMGGNNP LVVEQVADLD AAVYTIIQSA FISAGQRCTC ARRLLVPQGA WGDTLLARLV 310 320 330 340 350 360 AVSATIEVGA FDQQPAPFMG SVISLGAARA LMDAQQQLLA NGAVALLEMT QPQAQAALLT 370 380 390 400 410 420 PGILDVSAVA ERPDEELFGP LLQVIRYADF DDAIAEANNT QYGLAAGLLS DSAERYQQFW 430 440 450 460 470 480 LESRAGIVNW NKQLTGAASS APFGGVGASG NHRASAYYAA DYCAYPVASL ETPSLVLPSA LTPGVRLS

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000076 Genomic DNA. Translation: AAY93759.1.
RefSeq	YP_261596.1.
3D structure databases
SMR	Q4K837. Positions 3-462.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4K837.
Genome annotation databases
GeneID	3478627.
GenomeReviews	Gene locus PFL_4512 in contig CP000076_GR.
KEGG	pfl:PFL_4512.
NMPDR	fig|220664.3.peg.5007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	EMTQPQA.
PhylomeDB	Q4K837.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_4512-MONOMER.
Family and domain databases
HAMAP	MF_01174. Aldedh_AstD. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_PSEF5

Accession

Primary (citable) accession number: Q4K837

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)