Reviewed, UniProtKB/Swiss-Prot Q4K7Q8 (GCSP1_PSEF5)

Names and origin

Protein names

Recommended name:
    Glycine dehydrogenase [decarboxylating] 1
    EC=1.4.4.2
Alternative name(s):
    Glycine decarboxylase 1
    Glycine cleavage system P-protein 1

Gene names

Name:	gcvP1
Ordered Locus Names:	PFL_4641

Organism

Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) [Complete proteome] [HAMAP]

Taxonomic identifier

220664 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	951 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity.
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00711
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvP family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 951	951	Glycine dehydrogenase [decarboxylating] 1 HAMAP MF_00711		PRO_0000227116
Amino acid modifications
	Modified residue	703	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4K7Q8-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: BBF2ACC0CF69E95F	FASTA	951	102,473
	10 20 30 40 50 60 MTVNLSTANE FIARHIGPRQ GDEQAMLNSL GFDSLEALSA SVIPESIKGT SVLELGHGLS 70 80 90 100 110 120 EAQALASIKA IAARNQLFKT YIGQGYYNCH TPSPILRNLL ENPAWYTAYT PYQPEISQGR 130 140 150 160 170 180 LEALLNFQTL ISDLSGLPIA NASLLDEGTA AAEAMTFCKR LSKNKGSHAF FASQHCHPQT 190 200 210 220 230 240 LDVLRTRAEP LGINVVVGDE RKLTDVSPFF GALLQYPASN GDLFDYRELT ERFHAANALV 250 260 270 280 290 300 AVAADLLALT LLTPPGEFGA DVAIGSAQRF GVPLGFGGPH AAYFATRDAF KRDMPGRLVG 310 320 330 340 350 360 VSVDRFGKPA LRLAMQTREQ HIRREKATSN ICTAQVLLAN IASMYAVYHG PKGLTQIAQR 370 380 390 400 410 420 IHQLTAILAK GLVQLGLTVE QESFFDTLSL HTAGRTAALH DKARAQGINL RVIDAERLGL 430 440 450 460 470 480 SLDETTTQAD VETLWSLLAD GKPAPDFAAL AAAVTSGIPA ALARQSAILS HPVFNRYHSE 490 500 510 520 530 540 TELMRYLRKL ADKDLALDRT MIPLGSCTMK LNAASEMIPI TWAEFGALHP FAPAEQSAGY 550 560 570 580 590 600 QQLTTELEAM LCAATGYDAV SLQPNAGSQG EYAGLLAIRA YHQSRGDERR DICLIPSSAH 610 620 630 640 650 660 GTNPATANMA GMRVVVTACD ARGNVDIEDL RAKAIEHREH LAALMITYPS THGVFEEGIR 670 680 690 700 710 720 EICGIIHDNG GQVYIDGANM NAMVGLCAPG KFGGDVSHLN LHKTFCIPHG GGGPGVGPIG 730 740 750 760 770 780 VKSHLAPFLP GHAALENKKG AVCAAPFGSA SILPITWMYI SMMGGAGLKR ASQLAILNAN 790 800 810 820 830 840 YISRRLEEHY PVLYTGSNGL VAHECILDLR PLKDSSGISV DDVAKRLIDF GFHAPTMSFP 850 860 870 880 890 900 VAGTLMIEPT ESESKEELDR FCDAMIRIRE EIRAVENGAL DKDDNPLKNA PHTAAELVGE 910 920 930 940 950 WSHPYSREQA VYPVASLVEG KYWPPVGRVD NVFGDRNLVC ACPSIESYQD A

References

[1]

"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5." Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L. , Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. Nat. Biotechnol. 23:873-878(2005) [PubMed: 15980861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000076 Genomic DNA. Translation: AAY93888.1.
RefSeq	YP_261725.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3478756.
GenomeReviews	Gene locus PFL_4641 in contig CP000076_GR.
KEGG	pfl:PFL_4641.
NMPDR	fig|220664.3.peg.5278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4K7Q8.
OMA	Q4K7Q8. NRAESEM.
Enzyme and pathway databases
BioCyc	PFLU220664:PFL_4641-MON.
Family and domain databases
HAMAP	MF_00711. [Tree]
InterPro	IPR003437. GDC-P. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
TIGRFAMs	TIGR00461. gcvP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCSP1_PSEF5

Accession

Primary (citable) accession number: Q4K7Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)